Systematic evaluation of colorectal cancer organoid system by single-cell RNA-Seq analysis

• Published in: Genome Biology Vol. 23; no. 1; p. 106
• Main Authors: Wang, Rui, Mao, Yunuo, Wang, Wendong, Zhou, Xin, Wang, Wei, Gao, Shuai, Li, Jingyun, Wen, Lu, Fu, Wei, Tang, Fuchou
• Format: Journal Article
• Language: English
• Published: England BioMed Central 28.04.2022; BMC
• Subjects: Cancer; Cell culture; Colorectal cancer; Colorectal carcinoma; colorectal neoplasms; Colorectal Neoplasms - pathology; Culture; Culture media; DNA Methylation; Epithelial cells; epithelium; Gene expression; genome; Genomes; genomics; Humans; Molecular modelling; Mutation; Organoids; Organoids - metabolism; Organoids - pathology; Patients; sequence analysis; Sequence Analysis, RNA; Stem cells; transcriptome; Transcriptomes; Tumors

Patient-derived organoid culture is a powerful system for studying the molecular mechanisms of cancers, especially colorectal cancer (CRC), one of the most prevalent cancers worldwide. There are two main types of 3D culture methods for colonic cells, but the similarities and differences between gene...