Voltage-Dependent Modulation of Cardiac Ryanodine Receptors (RyR2) by Protamine

• Published in: PloS one Vol. 4; no. 12; p. e8315
• Main Authors: Diaz-Sylvester, Paula L., Copello, Julio A.
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 15.12.2009; Public Library of Science (PLoS)
• Subjects: Activation; Animals; Anticoagulants; Biophysics/Biomacromolecule-Ligand Interactions; Biophysics/Membrane Proteins and Energy Transduction; Calcium (reticular); Calcium - metabolism; Calcium channels; Cardiac muscle; Channel gating; Conductance; Coronary artery disease; Cytosol; Cytosol - drug effects; Cytosol - metabolism; Electric Conductivity; Electric potential; Electrostatic properties; Heart diseases; Histones; Ion Channel Gating - drug effects; Laboratory animals; Leak channels; Ligands; Lipid bilayers; Lipids; Magnesium; Microsomes; Modulation; Musculoskeletal system; Myocardium - metabolism; Physiology/Cardiovascular Physiology and Circulation; Physiology/Cell Signaling; Physiology/Membranes and Sorting; Physiology/Muscle and Connective Tissue; Polycations; Protamine; Protamines - pharmacology; Proteins; Receptors; Resistance; Rodents; Ryanodine Receptor Calcium Release Channel - metabolism; Ryanodine receptors; Sarcoplasmic reticulum; Scorpion Venoms - pharmacology; Sus scrofa; Voltage; United States > US; Illinois

It has been reported that protamine (>10 microg/ml) blocks single skeletal RyR1 channels and inhibits RyR1-mediated Ca2+ release from sarcoplasmic reticulum microsomes. We extended these studies to cardiac RyR2 reconstituted into planar lipid bilayers. We found that protamine (0.02-20 microg/ml)...