ADAMTS9-Regulated Pericellular Matrix Dynamics Governs Focal Adhesion-Dependent Smooth Muscle Differentiation

• Published in: Cell reports (Cambridge) Vol. 23; no. 2; pp. 485 - 498
• Main Authors: Mead, Timothy J., Du, Yaoyao, Nelson, Courtney M., Gueye, Ndeye-Aicha, Drazba, Judith, Dancevic, Carolyn M., Vankemmelbeke, Mireille, Buttle, David J., Apte, Suneel S.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 10.04.2018; Elsevier
• Subjects: Actins - metabolism; ADAMTS9 Protein - antagonists & inhibitors; ADAMTS9 Protein - genetics; ADAMTS9 Protein - metabolism; Animals; Cell Differentiation; Cell Nucleus - metabolism; Cytoskeleton - metabolism; extracellular matrix; Extracellular Matrix - metabolism; Female; focal adhesion; Focal Adhesions - metabolism; Humans; interference reflection microscopy; metalloprotease; Mice; Mice, Transgenic; Microfilament Proteins - metabolism; Muscle Proteins - metabolism; Myocytes, Smooth Muscle - cytology; Myocytes, Smooth Muscle - metabolism; myometrium; Myometrium - metabolism; Myometrium - pathology; parturition; Pregnancy; proteoglycan; proteolysis; RNA Interference; RNA, Small Interfering - metabolism; smooth muscle; uterus; Uterus - cytology; Versicans - metabolism; myometrium; focal adhesion; interference reflection microscopy; uterus; smooth muscle; metalloprotease; extracellular matrix; parturition; proteolysis; proteoglycan

Focal adhesions anchor cells to extracellular matrix (ECM) and direct assembly of a pre-stressed actin cytoskeleton. They act as a cellular sensor and regulator, linking ECM to the nucleus. Here, we identify proteolytic turnover of the anti-adhesive proteoglycan versican as a requirement for mainten...