Transgenic expression of human equilibrative nucleoside transporter 1 in mouse neurons

• Published in: Journal of neurochemistry Vol. 109; no. 2; pp. 562 - 572
• Main Authors: Parkinson, Fiona E, Xiong, Wei, Zamzow, Christina R, Chestley, Taeyo, Mizuno, Tooru, Duckworth, Mary Lynn
• Format: Journal Article
• Language: English
• Published: Oxford, UK Oxford, UK : Blackwell Publishing Ltd 01.04.2009; Blackwell Publishing Ltd; Wiley-Blackwell
• Subjects: adenosine; Adenosine - metabolism; Animals; behavior; Behavior, Animal - drug effects; Behavior, Animal - physiology; Biochemistry; Biological and medical sciences; caffeine; Caffeine - administration & dosage; Caffeine - antagonists & inhibitors; Cell physiology; Degenerative and inherited degenerative diseases of the nervous system. Leukodystrophies. Prion diseases; Equilibrative Nucleoside Transporter 1 - biosynthesis; Equilibrative Nucleoside Transporter 1 - genetics; Equilibrative Nucleoside Transporter 1 - physiology; ethanol; Ethanol - administration & dosage; Fundamental and applied biological sciences. Psychology; Gene expression; Gene Expression Regulation - drug effects; Gene Expression Regulation - physiology; Humans; Medical sciences; Membrane and intracellular transports; Mice; Mice, Transgenic; Molecular and cellular biology; Neurology; Neurons - drug effects; Neurons - metabolism; Neurons - physiology; nucleoside transport; PC12 Cells; Rats; Receptors, Purinergic P1 - biosynthesis; Receptors, Purinergic P1 - genetics; Rodents; Transgenic animals; Up-Regulation - genetics; Cerebellum; Heart; Cerebral cortex; Liver; Lung; Central nervous system; Respiratory system; ethanol; Kidney; Encephalon; Synaptosome; caffeine; behavior; Human; nucleoside transport; Digestive system; ENT1 nucleoside transporter; Rodentia; Basal ganglion; Corpus striatum; Striated muscle; adenosine; Vertebrata; Mammalia; Neuron; Urinary system; Mouse; Circulatory system; Subtype; Hippocampus

Transgenic mice that express human equilibrative nucleoside transporter subtype 1 (hENT1) under the control of a neuron-specific enolase promoter have been generated. Southern blot and PCR revealed the presence of the transgene in five founder mice. Mice from each founder line were examined by rever...