Transgenic expression of human equilibrative nucleoside transporter 1 in mouse neurons
Transgenic mice that express human equilibrative nucleoside transporter subtype 1 (hENT1) under the control of a neuron-specific enolase promoter have been generated. Southern blot and PCR revealed the presence of the transgene in five founder mice. Mice from each founder line were examined by rever...
Saved in:
| Published in | Journal of neurochemistry Vol. 109; no. 2; pp. 562 - 572 |
|---|---|
| Main Authors | , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
Oxford, UK
Oxford, UK : Blackwell Publishing Ltd
01.04.2009
Blackwell Publishing Ltd Wiley-Blackwell |
| Subjects | |
| Online Access | Get full text |
Cover
Loading…
| Abstract | Transgenic mice that express human equilibrative nucleoside transporter subtype 1 (hENT1) under the control of a neuron-specific enolase promoter have been generated. Southern blot and PCR revealed the presence of the transgene in five founder mice. Mice from each founder line were examined by reverse transcriptase (RT)-PCR and found to express hENT1 in RNA isolated from whole brain, cerebral cortex, striatum, hippocampus, and cerebellum but not liver, kidney, heart, lung or skeletal muscle. Cortical synaptosomes prepared from transgenic mice had significantly increased [³H]adenosine uptake and [³H]nitrobenzylthioinosine binding, relative to samples from wild-type mice. In behavioral tests, transgenic mice had altered responses to caffeine and ethanol, two drugs that inhibit and enhance, respectively, adenosine receptor activity. Caffeine-induced locomotor stimulation was attenuated whereas the hypnotic effect of ethanol was enhanced in transgenic mice. Caffeine was more potent in inhibiting ethanol-induced motor incoordination in wild-type than in transgenic mice. No differences in expression of mouse genes for adenosine receptors, nucleoside transporters, or purine metabolizing enzymes were detected by RT-PCR analyses. These data indicate that expression of hENT1 in neurons does not trigger adaptive changes in expression of adenosine-related genes. Instead, hENT1 expression affects dynamic changes in endogenous adenosine levels, as revealed by altered behavioral responses to drugs that affect adenosine receptor signalling. |
|---|---|
| AbstractList | Transgenic mice that express human equilibrative nucleoside transporter subtype 1 (hENT1) under the control of a neuron-specific enolase promoter have been generated. Southern blot and PCR revealed the presence of the transgene in five founder mice. Mice from each founder line were examined by reverse transcriptase (RT)-PCR and found to express hENT1 in RNA isolated from whole brain, cerebral cortex, striatum, hippocampus, and cerebellum but not liver, kidney, heart, lung or skeletal muscle. Cortical synaptosomes prepared from transgenic mice had significantly increased [(3)H]adenosine uptake and [(3)H]nitrobenzylthioinosine binding, relative to samples from wild-type mice. In behavioral tests, transgenic mice had altered responses to caffeine and ethanol, two drugs that inhibit and enhance, respectively, adenosine receptor activity. Caffeine-induced locomotor stimulation was attenuated whereas the hypnotic effect of ethanol was enhanced in transgenic mice. Caffeine was more potent in inhibiting ethanol-induced motor incoordination in wild-type than in transgenic mice. No differences in expression of mouse genes for adenosine receptors, nucleoside transporters, or purine metabolizing enzymes were detected by RT-PCR analyses. These data indicate that expression of hENT1 in neurons does not trigger adaptive changes in expression of adenosine-related genes. Instead, hENT1 expression affects dynamic changes in endogenous adenosine levels, as revealed by altered behavioral responses to drugs that affect adenosine receptor signalling. Transgenic mice that express human equilibrative nucleoside transporter subtype 1 (hENT1) under the control of a neuron-specific enolase promoter have been generated. Southern blot and PCR revealed the presence of the transgene in five founder mice. Mice from each founder line were examined by reverse transcriptase (RT)-PCR and found to express hENT1 in RNA isolated from whole brain, cerebral cortex, striatum, hippocampus, and cerebellum but not liver, kidney, heart, lung or skeletal muscle. Cortical synaptosomes prepared from transgenic mice had significantly increased [³H]adenosine uptake and [³H]nitrobenzylthioinosine binding, relative to samples from wild-type mice. In behavioral tests, transgenic mice had altered responses to caffeine and ethanol, two drugs that inhibit and enhance, respectively, adenosine receptor activity. Caffeine-induced locomotor stimulation was attenuated whereas the hypnotic effect of ethanol was enhanced in transgenic mice. Caffeine was more potent in inhibiting ethanol-induced motor incoordination in wild-type than in transgenic mice. No differences in expression of mouse genes for adenosine receptors, nucleoside transporters, or purine metabolizing enzymes were detected by RT-PCR analyses. These data indicate that expression of hENT1 in neurons does not trigger adaptive changes in expression of adenosine-related genes. Instead, hENT1 expression affects dynamic changes in endogenous adenosine levels, as revealed by altered behavioral responses to drugs that affect adenosine receptor signalling. Transgenic mice that express human equilibrative nucleoside transporter subtype 1 (hENT1) under the control of a neuron-specific enolase promoter have been generated. Southern blot and PCR revealed the presence of the transgene in five founder mice. Mice from each founder line were examined by reverse transcriptase (RT)-PCR and found to express hENT1 in RNA isolated from whole brain, cerebral cortex, striatum, hippocampus, and cerebellum but not liver, kidney, heart, lung or skeletal muscle. Cortical synaptosomes prepared from transgenic mice had significantly increased [3H]adenosine uptake and [3H]nitrobenzylthioinosine binding, relative to samples from wild-type mice. In behavioral tests, transgenic mice had altered responses to caffeine and ethanol, two drugs that inhibit and enhance, respectively, adenosine receptor activity. Caffeine-induced locomotor stimulation was attenuated whereas the hypnotic effect of ethanol was enhanced in transgenic mice. Caffeine was more potent in inhibiting ethanol-induced motor incoordination in wild-type than in transgenic mice. No differences in expression of mouse genes for adenosine receptors, nucleoside transporters, or purine metabolizing enzymes were detected by RT-PCR analyses. These data indicate that expression of hENT1 in neurons does not trigger adaptive changes in expression of adenosine-related genes. Instead, hENT1 expression affects dynamic changes in endogenous adenosine levels, as revealed by altered behavioral responses to drugs that affect adenosine receptor signalling. [PUBLICATION ABSTRACT] Transgenic mice that express human equilibrative nucleoside transporter subtype 1 (hENT1) under the control of a neuron‐specific enolase promoter have been generated. Southern blot and PCR revealed the presence of the transgene in five founder mice. Mice from each founder line were examined by reverse transcriptase (RT)‐PCR and found to express hENT1 in RNA isolated from whole brain, cerebral cortex, striatum, hippocampus, and cerebellum but not liver, kidney, heart, lung or skeletal muscle. Cortical synaptosomes prepared from transgenic mice had significantly increased [3H]adenosine uptake and [3H]nitrobenzylthioinosine binding, relative to samples from wild‐type mice. In behavioral tests, transgenic mice had altered responses to caffeine and ethanol, two drugs that inhibit and enhance, respectively, adenosine receptor activity. Caffeine‐induced locomotor stimulation was attenuated whereas the hypnotic effect of ethanol was enhanced in transgenic mice. Caffeine was more potent in inhibiting ethanol‐induced motor incoordination in wild‐type than in transgenic mice. No differences in expression of mouse genes for adenosine receptors, nucleoside transporters, or purine metabolizing enzymes were detected by RT‐PCR analyses. These data indicate that expression of hENT1 in neurons does not trigger adaptive changes in expression of adenosine‐related genes. Instead, hENT1 expression affects dynamic changes in endogenous adenosine levels, as revealed by altered behavioral responses to drugs that affect adenosine receptor signalling. Transgenic mice that express human equilibrative nucleoside transporter subtype 1 (hENT1) under the control of a neuron‐specific enolase promoter have been generated. Southern blot and PCR revealed the presence of the transgene in five founder mice. Mice from each founder line were examined by reverse transcriptase (RT)‐PCR and found to express hENT1 in RNA isolated from whole brain, cerebral cortex, striatum, hippocampus, and cerebellum but not liver, kidney, heart, lung or skeletal muscle. Cortical synaptosomes prepared from transgenic mice had significantly increased [ 3 H]adenosine uptake and [ 3 H]nitrobenzylthioinosine binding, relative to samples from wild‐type mice. In behavioral tests, transgenic mice had altered responses to caffeine and ethanol, two drugs that inhibit and enhance, respectively, adenosine receptor activity. Caffeine‐induced locomotor stimulation was attenuated whereas the hypnotic effect of ethanol was enhanced in transgenic mice. Caffeine was more potent in inhibiting ethanol‐induced motor incoordination in wild‐type than in transgenic mice. No differences in expression of mouse genes for adenosine receptors, nucleoside transporters, or purine metabolizing enzymes were detected by RT‐PCR analyses. These data indicate that expression of hENT1 in neurons does not trigger adaptive changes in expression of adenosine‐related genes. Instead, hENT1 expression affects dynamic changes in endogenous adenosine levels, as revealed by altered behavioral responses to drugs that affect adenosine receptor signalling. AbstractTransgenic mice that express human equilibrative nucleoside transporter subtype 1 (hENT1) under the control of a neuron-specific enolase promoter have been generated. Southern blot and PCR revealed the presence of the transgene in five founder mice. Mice from each founder line were examined by reverse transcriptase (RT)-PCR and found to express hENT1 in RNA isolated from whole brain, cerebral cortex, striatum, hippocampus, and cerebellum but not liver, kidney, heart, lung or skeletal muscle. Cortical synaptosomes prepared from transgenic mice had significantly increased [3H]adenosine uptake and [3H]nitrobenzylthioinosine binding, relative to samples from wild-type mice. In behavioral tests, transgenic mice had altered responses to caffeine and ethanol, two drugs that inhibit and enhance, respectively, adenosine receptor activity. Caffeine-induced locomotor stimulation was attenuated whereas the hypnotic effect of ethanol was enhanced in transgenic mice. Caffeine was more potent in inhibiting ethanol-induced motor incoordination in wild-type than in transgenic mice. No differences in expression of mouse genes for adenosine receptors, nucleoside transporters, or purine metabolizing enzymes were detected by RT-PCR analyses. These data indicate that expression of hENT1 in neurons does not trigger adaptive changes in expression of adenosine-related genes. Instead, hENT1 expression affects dynamic changes in endogenous adenosine levels, as revealed by altered behavioral responses to drugs that affect adenosine receptor signalling. |
| Author | Zamzow, Christina R Duckworth, Mary Lynn Chestley, Taeyo Parkinson, Fiona E Mizuno, Tooru Xiong, Wei |
| Author_xml | – sequence: 1 fullname: Parkinson, Fiona E – sequence: 2 fullname: Xiong, Wei – sequence: 3 fullname: Zamzow, Christina R – sequence: 4 fullname: Chestley, Taeyo – sequence: 5 fullname: Mizuno, Tooru – sequence: 6 fullname: Duckworth, Mary Lynn |
| BackLink | http://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=21283782$$DView record in Pascal Francis https://www.ncbi.nlm.nih.gov/pubmed/19222701$$D View this record in MEDLINE/PubMed |
| BookMark | eNqNkk9vFCEYxompsdvqV1BiorcZgWEYOHgwm9Y_afRg65UwzEtlMwNb2NHtt5dxNzXxolwg8HtenpeHM3QSYgCEMCU1LePNpqa8oxWnraoZIaomrVK03j9Cq4eDE7QihLGqIZydorOcN4RQwQV9gk6pYox1hK7Qt-tkQr6F4C2G_TZBzj4GHB3-Pk8mYLib_ej7ZHb-B-Aw2xFi9gPg3aLbxrSDhCn2AU9xzoWAOcWQn6LHzowZnh3nc3RzeXG9_lBdfXn_cf3uqrKtamnVu9Z1veXOtLwfuDQ9gGyks7JpmLRll0NbGjJiGNxAFOsUFb0Q4JwgkvXNOXp9qLtN8W6GvNOTzxbG0QQofrToiBJSdf8EGWm5pIQV8OVf4CbOKZQmCiPalgmuCiQPkE0x5wROb5OfTLrXlOglIb3RSxB6CUIvCenfCel9kT4_1p_7CYY_wmMkBXh1BEy2ZnTlna3PDxyjTDadXIy-PXA__Qj3_21Af_q8XlZF_-KgdyZqc5vKHTdfWTFQfgnpSp_NL-hOt5M |
| CODEN | JONRA9 |
| CitedBy_id | crossref_primary_10_1007_s12017_014_8338_2 crossref_primary_10_1111_j_1530_0277_2010_01379_x crossref_primary_10_1038_srep27758 crossref_primary_10_1089_jcr_2011_0017 crossref_primary_10_1111_j_1530_0277_2010_01153_x crossref_primary_10_1007_s12311_017_0866_5 crossref_primary_10_1016_j_bbr_2011_05_023 crossref_primary_10_1016_j_biopsych_2011_02_013 crossref_primary_10_1007_s12031_013_0064_y crossref_primary_10_1152_ajpheart_00711_2009 crossref_primary_10_1038_aps_2012_127 crossref_primary_10_1111_j_1471_4159_2011_07263_x crossref_primary_10_1186_2040_7378_4_4 crossref_primary_10_1124_jpet_116_232231 crossref_primary_10_1371_journal_pone_0020934 crossref_primary_10_1111_j_1471_4159_2011_07242_x crossref_primary_10_1016_j_neulet_2010_12_033 crossref_primary_10_1016_S1995_7645_13_60162_5 crossref_primary_10_1016_j_neuropharm_2012_02_022 crossref_primary_10_1111_j_1530_0277_2011_01722_x crossref_primary_10_1007_s00213_016_4212_2 crossref_primary_10_1016_j_neulet_2010_05_079 crossref_primary_10_1042_BJ20130231 |
| Cites_doi | 10.1111/j.1471-4159.2005.03312.x 10.1016/0896-6273(90)90308-3 10.1074/jbc.M513825200 10.1006/meth.2001.1262 10.1016/S0021-9258(19)38732-0 10.1146/annurev.pharmtox.45.120403.095731 10.1073/pnas.92.10.4397 10.1161/01.RES.0000238359.18495.42 10.1042/bj2620109 10.1111/j.2042-7158.1988.tb05282.x 10.1016/S0306-4522(00)00479-6 10.1016/0378-1119(87)90218-6 10.1016/S0006-8993(96)01263-2 10.1111/j.1600-0773.1995.tb00111.x 10.1007/s00424-003-1107-y 10.1111/j.1471-4159.1985.tb04020.x 10.1152/ajprenal.00007.2007 10.1152/ajpcell.1992.262.5.C1175 10.1126/science.287.5455.1056 10.1016/S0361-9230(01)00552-4 10.1016/0006-8993(94)90767-6 10.1074/jbc.M414337200 10.1097/00004647-200108000-00005 10.1016/S0074-7742(05)63007-3 10.1111/j.1601-183X.2007.00311.x 10.1007/s00424-003-1103-2 10.1146/annurev.neuro.24.1.31 10.1038/sj.bjp.0703170 10.1128/JVI.75.11.5328-5334.2001 10.1016/S0169-328X(96)00244-6 10.1523/JNEUROSCI.22-23-10487.2002 10.1038/nn1288 10.1016/0896-6273(95)90105-1 10.1080/09687860701210617 10.1139/o02-143 |
| ContentType | Journal Article |
| Copyright | 2009 The Authors. Journal Compilation © 2009 International Society for Neurochemistry 2009 INIST-CNRS Journal compilation © 2009 International Society for Neurochemistry |
| Copyright_xml | – notice: 2009 The Authors. Journal Compilation © 2009 International Society for Neurochemistry – notice: 2009 INIST-CNRS – notice: Journal compilation © 2009 International Society for Neurochemistry |
| DBID | FBQ IQODW CGR CUY CVF ECM EIF NPM AAYXX CITATION 7QR 7TK 7U7 7U9 8FD C1K FR3 H94 P64 7QO 7TM RC3 7X8 |
| DOI | 10.1111/j.1471-4159.2009.05991.x |
| DatabaseName | AGRIS Pascal-Francis Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed CrossRef Chemoreception Abstracts Neurosciences Abstracts Toxicology Abstracts Virology and AIDS Abstracts Technology Research Database Environmental Sciences and Pollution Management Engineering Research Database AIDS and Cancer Research Abstracts Biotechnology and BioEngineering Abstracts Biotechnology Research Abstracts Nucleic Acids Abstracts Genetics Abstracts MEDLINE - Academic |
| DatabaseTitle | MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) CrossRef Virology and AIDS Abstracts Technology Research Database Toxicology Abstracts AIDS and Cancer Research Abstracts Chemoreception Abstracts Engineering Research Database Neurosciences Abstracts Biotechnology and BioEngineering Abstracts Environmental Sciences and Pollution Management Genetics Abstracts Biotechnology Research Abstracts Nucleic Acids Abstracts MEDLINE - Academic |
| DatabaseTitleList | MEDLINE - Academic Virology and AIDS Abstracts CrossRef Genetics Abstracts MEDLINE |
| Database_xml | – sequence: 1 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: EIF name: MEDLINE url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search sourceTypes: Index Database – sequence: 3 dbid: FBQ name: AGRIS url: http://www.fao.org/agris/Centre.asp?Menu_1ID=DB&Menu_2ID=DB1&Language=EN&Content=http://www.fao.org/agris/search?Language=EN sourceTypes: Publisher |
| DeliveryMethod | fulltext_linktorsrc |
| Discipline | Anatomy & Physiology Chemistry |
| EISSN | 1471-4159 |
| EndPage | 572 |
| ExternalDocumentID | 1664204361 10_1111_j_1471_4159_2009_05991_x 19222701 21283782 JNC5991 US201301607526 |
| Genre | article Research Support, Non-U.S. Gov't Journal Article Comparative Study Feature |
| GroupedDBID | --- -~X .3N .55 .GA .GJ .Y3 05W 0R~ 10A 1OB 1OC 24P 29L 2WC 31~ 33P 36B 3SF 4.4 41~ 50Y 50Z 51W 51X 52M 52N 52O 52P 52R 52S 52T 52U 52V 52W 52X 53G 5GY 5HH 5LA 5RE 5VS 66C 702 7PT 8-0 8-1 8-3 8-4 8-5 8UM 930 A01 A03 AAESR AAEVG AAHHS AAJUZ AANLZ AAONW AASGY AAVGM AAXRX AAYJJ AAZKR ABCQN ABCUV ABCVL ABEML ABHUG ABIVO ABLJU ABPTK ABPVW ABQWH ABWRO ABXGK ACAHQ ACBWZ ACCFJ ACCZN ACFBH ACGFO ACGFS ACGOD ACGOF ACIWK ACMXC ACNCT ACPOU ACPRK ACSCC ACSMX ACXBN ACXME ACXQS ADAWD ADBBV ADBTR ADDAD ADEOM ADIZJ ADKYN ADMGS ADOZA ADXAS ADZMN AEEZP AEGXH AEIGN AEIMD AENEX AEQDE AEUQT AEUYR AFBPY AFEBI AFFPM AFGKR AFPWT AFRAH AFVGU AFZJQ AGJLS AHEFC AI. AIACR AIAGR AIURR AIWBW AJBDE ALAGY ALMA_UNASSIGNED_HOLDINGS ALUQN AMBMR AMYDB ASPBG ATUGU AVWKF AZBYB AZFZN AZVAB BAFTC BAWUL BDRZF BFHJK BHBCM BMXJE BROTX BRXPI BY8 C45 CAG COF CS3 D-6 D-7 D-E D-F DC6 DCZOG DIK DPXWK DR2 DRFUL DRMAN DRSTM DU5 E3Z EBS EJD EMOBN ESX EX3 F00 F01 F04 F5P FBQ FEDTE FIJ FUBAC FZ0 G-S G.N GAKWD GODZA GX1 H.X HF~ HH5 HVGLF HZI HZ~ IH2 IHE IPNFZ IX1 J0M K48 KBYEO LATKE LC2 LC3 LEEKS LH4 LITHE LOXES LP6 LP7 LUTES LW6 LYRES MEWTI MK4 MRFUL MRMAN MRSTM MSFUL MSMAN MSSTM MVM MXFUL MXMAN MXSTM N04 N05 N9A NF~ O66 O9- OK1 OVD P2P P2W P2X P2Z P4B P4D PALCI PQQKQ Q.N Q11 QB0 R.K RIWAO RJQFR ROL RX1 SAMSI SUPJJ TEORI TWZ UB1 V8K VH1 W8V W99 WBKPD WIH WIJ WIK WIN WNSPC WOHZO WOW WQJ WRC WUP WXI WXSBR WYISQ X7M XG1 XJT YFH YNH YOC YUY ZA5 ZGI ZXP ZZTAW ~IA ~KM ~WT AHBTC AITYG HGLYW OIG 08R AAUGY IQODW CGR CUY CVF ECM EIF NPM AAMNL AAYXX ACRPL CITATION 7QR 7TK 7U7 7U9 8FD C1K FR3 H94 P64 7QO 7TM RC3 7X8 |
| ID | FETCH-LOGICAL-c5951-bf5f7bc4fa54bd48abee838fc83328ca544e5471a6ddfd0927916b66eff6082b3 |
| IEDL.DBID | DR2 |
| ISSN | 0022-3042 |
| IngestDate | Wed Dec 04 03:56:19 EST 2024 Wed Dec 04 05:24:28 EST 2024 Thu Oct 10 21:59:37 EDT 2024 Fri Dec 06 03:00:43 EST 2024 Sat Sep 28 07:54:36 EDT 2024 Sun Oct 22 16:05:32 EDT 2023 Sat Aug 24 00:57:23 EDT 2024 Wed Dec 27 19:12:14 EST 2023 |
| IsDoiOpenAccess | false |
| IsOpenAccess | true |
| IsPeerReviewed | true |
| IsScholarly | true |
| Issue | 2 |
| Keywords | Cerebellum Heart Cerebral cortex Liver Lung Central nervous system Respiratory system ethanol Kidney Encephalon Synaptosome caffeine behavior Human nucleoside transport Digestive system ENT1 nucleoside transporter Rodentia Basal ganglion Corpus striatum Striated muscle adenosine Vertebrata Mammalia Neuron Urinary system Mouse Circulatory system Subtype Hippocampus |
| Language | English |
| License | CC BY 4.0 http://onlinelibrary.wiley.com/termsAndConditions#vor |
| LinkModel | DirectLink |
| MergedId | FETCHMERGED-LOGICAL-c5951-bf5f7bc4fa54bd48abee838fc83328ca544e5471a6ddfd0927916b66eff6082b3 |
| Notes | http://dx.doi.org/10.1111/j.1471-4159.2009.05991.x ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
| OpenAccessLink | https://doi.org/10.1111/j.1471-4159.2009.05991.x |
| PMID | 19222701 |
| PQID | 206552649 |
| PQPubID | 31528 |
| PageCount | 11 |
| ParticipantIDs | proquest_miscellaneous_67096897 proquest_miscellaneous_20548102 proquest_journals_206552649 crossref_primary_10_1111_j_1471_4159_2009_05991_x pubmed_primary_19222701 pascalfrancis_primary_21283782 wiley_primary_10_1111_j_1471_4159_2009_05991_x_JNC5991 fao_agris_US201301607526 |
| PublicationCentury | 2000 |
| PublicationDate | April 2009 |
| PublicationDateYYYYMMDD | 2009-04-01 |
| PublicationDate_xml | – month: 04 year: 2009 text: April 2009 |
| PublicationDecade | 2000 |
| PublicationPlace | Oxford, UK |
| PublicationPlace_xml | – name: Oxford, UK – name: Oxford – name: England – name: New York |
| PublicationTitle | Journal of neurochemistry |
| PublicationTitleAlternate | J Neurochem |
| PublicationYear | 2009 |
| Publisher | Oxford, UK : Blackwell Publishing Ltd Blackwell Publishing Ltd Wiley-Blackwell |
| Publisher_xml | – name: Oxford, UK : Blackwell Publishing Ltd – name: Blackwell Publishing Ltd – name: Wiley-Blackwell |
| References | 2001; 102 1995; 92 1992; 262 1995; 15 2006; 99 1993; 44 2004; 7 1995; 76 2002; 80 1985; 45 1991; 259 2001; 24 2004; 447 1990; 265 2001; 25 2001; 21 2005; 280 2005a; 63 1994; 666 2007; 293 1987; 60 1989; 262 2000; 129 2002; 22 1986 2007; 6 1988; 40 2000; 287 2005b; 45 2006; 281 2005; 94 1990; 255 2001; 55 1997; 749 2007; 24 1996; 42 2001; 75 1990; 5 e_1_2_7_6_1 e_1_2_7_5_1 e_1_2_7_4_1 e_1_2_7_3_1 Crawford C. R. (e_1_2_7_11_1) 1990; 265 e_1_2_7_9_1 e_1_2_7_8_1 e_1_2_7_19_1 e_1_2_7_18_1 e_1_2_7_17_1 e_1_2_7_16_1 e_1_2_7_40_1 e_1_2_7_2_1 e_1_2_7_14_1 e_1_2_7_13_1 e_1_2_7_12_1 e_1_2_7_10_1 e_1_2_7_26_1 e_1_2_7_29_1 Dar M. S. (e_1_2_7_15_1) 1990; 255 Krauss S. W. (e_1_2_7_31_1) 1993; 44 Carmichael F. J. (e_1_2_7_7_1) 1991; 259 e_1_2_7_30_1 e_1_2_7_25_1 e_1_2_7_24_1 e_1_2_7_32_1 e_1_2_7_23_1 e_1_2_7_33_1 e_1_2_7_22_1 e_1_2_7_34_1 e_1_2_7_21_1 Hogan B. (e_1_2_7_28_1) 1986 e_1_2_7_35_1 e_1_2_7_20_1 e_1_2_7_36_1 e_1_2_7_37_1 e_1_2_7_38_1 e_1_2_7_39_1 Hammond J. R. (e_1_2_7_27_1) 1989; 262 |
| References_xml | – volume: 6 start-page: 776 year: 2007 article-title: The type 1 equilibrative nucleoside transporter regulates anxiety‐like behavior in mice publication-title: Genes Brain Behav. – volume: 25 start-page: 402 year: 2001 end-page: 408 article-title: Analysis of relative gene expression data using real‐time quantitative PCR and the 2(‐Delta Delta C(T)) Method publication-title: Methods – volume: 76 start-page: 93 year: 1995 end-page: 101 article-title: Astra Award Lecture. Adenosine, adenosine receptors and the actions of caffeine publication-title: Pharmacol. Toxicol. – volume: 44 start-page: 1021 year: 1993 end-page: 1026 article-title: Inhibition of adenosine uptake by ethanol is specific for one class of nucleoside transporters publication-title: Mol. Pharmacol. – volume: 63 start-page: 191 year: 2005a end-page: 270 article-title: Adenosine and brain function publication-title: Int. Rev. Neurobiol. – volume: 15 start-page: 1183 year: 1995 end-page: 1191 article-title: Neuron‐specific expression of a hamster prion protein minigene in transgenic mice induces susceptibility to hamster scrapie agent publication-title: Neuron – volume: 75 start-page: 5328 year: 2001 end-page: 5334 article-title: Efficient transmission of two different sheep scrapie isolates in transgenic mice expressing the ovine PrP gene publication-title: J. Virol. – volume: 262 start-page: C1175 year: 1992 end-page: C1180 article-title: Ethanol metabolism and inhibition of nucleoside uptake lead to increased extracellular adenosine in hepatocytes publication-title: Am. J. Physiol. – volume: 42 start-page: 358 year: 1996 end-page: 361 article-title: Demonstration of the existence of mRNAs encoding the N1/ and N2/ sodium/nucleoside cotransporters in rat brain publication-title: Mol. Brain Res. – volume: 287 start-page: 1056 year: 2000 end-page: 1060 article-title: Ethanol‐induced apoptotic neurodegeneration and fetal alcohol syndrome publication-title: Science – volume: 265 start-page: 9732 year: 1990 end-page: 9736 article-title: Nucleoside transport in L1210 murine leukemia cells. Evidence for three transporters publication-title: J. Biol. Chem. – volume: 447 start-page: 735 year: 2004 end-page: 743 article-title: The equilibrative nucleoside transporter family, SLC29 publication-title: Pflugers Arch. – volume: 447 start-page: 728 year: 2004 end-page: 734 article-title: The concentrative nucleoside transporter family, SLC28 publication-title: Pflugers Arch. – year: 1986 – volume: 24 start-page: 31 year: 2001 end-page: 55 article-title: The role and regulation of adenosine in the central nervous system publication-title: Annu. Rev. Neurosci. – volume: 5 start-page: 187 year: 1990 end-page: 197 article-title: Transgenic mice expressing beta‐galactosidase in mature neurons under neuron‐specific enolase promoter control publication-title: Neuron – volume: 129 start-page: 1465 year: 2000 end-page: 1473 article-title: The stimulant effects of caffeine on locomotor behaviour in mice are mediated through its blockade of adenosine A(2A) receptors publication-title: Br. J. Pharmacol. – volume: 7 start-page: 855 year: 2004 end-page: 861 article-title: The type 1 equilibrative nucleoside transporter regulates ethanol intoxication and preference publication-title: Nat. Neurosci. – volume: 92 start-page: 4397 year: 1995 end-page: 4401 article-title: Bcl‐2 transgene expression can protect neurons against developmental and induced cell death publication-title: Proc. Natl Acad. Sci. USA – volume: 21 start-page: 929 year: 2001 end-page: 936 article-title: Cloned blood‐brain barrier adenosine transporter is identical to the rat concentrative Na+ nucleoside cotransporter CNT2 publication-title: J. Cereb. Blood Flow Metab. – volume: 24 start-page: 294 year: 2007 end-page: 303 article-title: Differential regulation of mouse equilibrative nucleoside transporter 1 (mENT1) splice variants by protein kinase CK2 publication-title: Mol. Membr. Biol. – volume: 40 start-page: 482 year: 1988 end-page: 487 article-title: The biphasic effects of centrally and peripherally administered caffeine on ethanol‐induced motor incoordination in mice publication-title: J. Pharm. Pharmacol. – volume: 55 start-page: 513 year: 2001 end-page: 520 article-title: Modulation of ethanol‐induced motor incoordination by mouse striatal A(1) adenosinergic receptor publication-title: Brain Res. Bull. – volume: 262 start-page: 109 year: 1989 end-page: 118 article-title: Solubilization and reconstitution of a nucleoside‐transport system from Ehrlich ascites‐tumour cells publication-title: Biochem. J. – volume: 259 start-page: 403 year: 1991 end-page: 408 article-title: Central nervous system effects of acetate: contribution to the central effects of ethanol publication-title: J. Pharmacol. Exp. Ther. – volume: 22 start-page: 10487 year: 2002 end-page: 10493 article-title: Low ethanol sensitivity and increased ethanol consumption in mice lacking adenosine A2A receptors publication-title: J. Neurosci. – volume: 749 start-page: 263 year: 1997 end-page: 274 article-title: Mouse cerebellar adenosinergic modulation of ethanol‐induced motor incoordination: possible involvement of cAMP publication-title: Brain Res. – volume: 45 start-page: 385 year: 2005b end-page: 412 article-title: Actions of adenosine at its receptors in the CNS: insights from knockouts and drugs publication-title: Annu. Rev. Pharmacol. Toxicol. – volume: 666 start-page: 151 year: 1994 end-page: 167 article-title: Synaptotrophic effects of human amyloid beta protein precursors in the cortex of transgenic mice publication-title: Brain Res. – volume: 281 start-page: 16700 year: 2006 end-page: 16706 article-title: Identification of the mitochondrial targeting signal of the human equilibrative nucleoside transporter 1 (hENT1): implications for interspecies differences in mitochondrial toxicity of fialuridine publication-title: J. Biol. Chem. – volume: 60 start-page: 103 year: 1987 end-page: 113 article-title: The structure and expression of neuron‐specific enolase gene publication-title: Gene – volume: 293 start-page: F200 year: 2007 end-page: F211 article-title: Localization of broadly selective equilibrative and concentrative nucleoside transporters, hENT1 and hCNT3, in human kidney publication-title: Am. J. Physiol. Renal Physiol. – volume: 45 start-page: 527 year: 1985 end-page: 535 article-title: Species differences in the binding of [3H]nitrobenzylthioinosine to the nucleoside transport system in mammalian central nervous system membranes: evidence for interconvertible conformations of the binding site/transporter complex publication-title: J. Neurochem. – volume: 94 start-page: 1420 year: 2005 end-page: 1426 article-title: Polarized distribution of nucleoside transporters in rat brain endothelial and choroid plexus epithelial cells publication-title: J. Neurochem. – volume: 80 start-page: 655 year: 2002 end-page: 665 article-title: Transport characteristics of HL‐1 cells: a new model for the study of adenosine physiology in cardiomyocytes publication-title: Biochem. Cell Biol. – volume: 255 start-page: 1202 year: 1990 end-page: 1209 article-title: Central adenosinergic system involvement in ethanol‐induced motor incoordination in mice publication-title: J. Pharmacol. Exp. Ther. – volume: 102 start-page: 307 year: 2001 end-page: 317 article-title: A gain‐of‐function mutation in the sodium channel gene Scn2a results in seizures and behavioral abnormalities publication-title: Neuroscience – volume: 280 start-page: 15880 year: 2005 end-page: 15887 article-title: Functional characterization of novel human and mouse equilibrative nucleoside transporters (hENT3 and mENT3) located in intracellular membranes publication-title: J. Biol. Chem. – volume: 99 start-page: 510 year: 2006 end-page: 519 article-title: Distribution and functional characterization of equilibrative nucleoside transporter‐4, a novel cardiac adenosine transporter activated at acidic pH publication-title: Circ. Res. – ident: e_1_2_7_39_1 doi: 10.1111/j.1471-4159.2005.03312.x – ident: e_1_2_7_21_1 doi: 10.1016/0896-6273(90)90308-3 – ident: e_1_2_7_32_1 doi: 10.1074/jbc.M513825200 – ident: e_1_2_7_34_1 doi: 10.1006/meth.2001.1262 – volume: 265 start-page: 9732 year: 1990 ident: e_1_2_7_11_1 article-title: Nucleoside transport in L1210 murine leukemia cells. Evidence for three transporters publication-title: J. Biol. Chem. doi: 10.1016/S0021-9258(19)38732-0 contributor: fullname: Crawford C. R. – ident: e_1_2_7_24_1 doi: 10.1146/annurev.pharmtox.45.120403.095731 – ident: e_1_2_7_20_1 doi: 10.1073/pnas.92.10.4397 – ident: e_1_2_7_5_1 doi: 10.1161/01.RES.0000238359.18495.42 – volume: 262 start-page: 109 year: 1989 ident: e_1_2_7_27_1 article-title: Solubilization and reconstitution of a nucleoside‐transport system from Ehrlich ascites‐tumour cells publication-title: Biochem. J. doi: 10.1042/bj2620109 contributor: fullname: Hammond J. R. – ident: e_1_2_7_14_1 doi: 10.1111/j.2042-7158.1988.tb05282.x – ident: e_1_2_7_30_1 doi: 10.1016/S0306-4522(00)00479-6 – volume: 259 start-page: 403 year: 1991 ident: e_1_2_7_7_1 article-title: Central nervous system effects of acetate: contribution to the central effects of ethanol publication-title: J. Pharmacol. Exp. Ther. contributor: fullname: Carmichael F. J. – ident: e_1_2_7_40_1 doi: 10.1016/0378-1119(87)90218-6 – ident: e_1_2_7_16_1 doi: 10.1016/S0006-8993(96)01263-2 – ident: e_1_2_7_22_1 doi: 10.1111/j.1600-0773.1995.tb00111.x – ident: e_1_2_7_25_1 doi: 10.1007/s00424-003-1107-y – ident: e_1_2_7_26_1 doi: 10.1111/j.1471-4159.1985.tb04020.x – ident: e_1_2_7_13_1 doi: 10.1152/ajprenal.00007.2007 – ident: e_1_2_7_37_1 doi: 10.1152/ajpcell.1992.262.5.C1175 – ident: e_1_2_7_29_1 doi: 10.1126/science.287.5455.1056 – ident: e_1_2_7_17_1 doi: 10.1016/S0361-9230(01)00552-4 – ident: e_1_2_7_35_1 doi: 10.1016/0006-8993(94)90767-6 – ident: e_1_2_7_4_1 doi: 10.1074/jbc.M414337200 – ident: e_1_2_7_33_1 doi: 10.1097/00004647-200108000-00005 – ident: e_1_2_7_23_1 doi: 10.1016/S0074-7742(05)63007-3 – ident: e_1_2_7_9_1 doi: 10.1111/j.1601-183X.2007.00311.x – ident: e_1_2_7_3_1 doi: 10.1007/s00424-003-1103-2 – ident: e_1_2_7_18_1 doi: 10.1146/annurev.neuro.24.1.31 – ident: e_1_2_7_19_1 doi: 10.1038/sj.bjp.0703170 – ident: e_1_2_7_12_1 doi: 10.1128/JVI.75.11.5328-5334.2001 – ident: e_1_2_7_2_1 doi: 10.1016/S0169-328X(96)00244-6 – volume: 44 start-page: 1021 year: 1993 ident: e_1_2_7_31_1 article-title: Inhibition of adenosine uptake by ethanol is specific for one class of nucleoside transporters publication-title: Mol. Pharmacol. contributor: fullname: Krauss S. W. – ident: e_1_2_7_36_1 doi: 10.1523/JNEUROSCI.22-23-10487.2002 – ident: e_1_2_7_10_1 doi: 10.1038/nn1288 – volume: 255 start-page: 1202 year: 1990 ident: e_1_2_7_15_1 article-title: Central adenosinergic system involvement in ethanol‐induced motor incoordination in mice publication-title: J. Pharmacol. Exp. Ther. contributor: fullname: Dar M. S. – ident: e_1_2_7_38_1 doi: 10.1016/0896-6273(95)90105-1 – ident: e_1_2_7_6_1 doi: 10.1080/09687860701210617 – ident: e_1_2_7_8_1 doi: 10.1139/o02-143 – volume-title: Manipulating the Mouse Embryo. A Laboratory Manual year: 1986 ident: e_1_2_7_28_1 contributor: fullname: Hogan B. |
| SSID | ssj0016461 |
| Score | 2.1235583 |
| Snippet | Transgenic mice that express human equilibrative nucleoside transporter subtype 1 (hENT1) under the control of a neuron-specific enolase promoter have been... Transgenic mice that express human equilibrative nucleoside transporter subtype 1 (hENT1) under the control of a neuron‐specific enolase promoter have been... AbstractTransgenic mice that express human equilibrative nucleoside transporter subtype 1 (hENT1) under the control of a neuron-specific enolase promoter have... |
| SourceID | proquest crossref pubmed pascalfrancis wiley fao |
| SourceType | Aggregation Database Index Database Publisher |
| StartPage | 562 |
| SubjectTerms | adenosine Adenosine - metabolism Animals behavior Behavior, Animal - drug effects Behavior, Animal - physiology Biochemistry Biological and medical sciences caffeine Caffeine - administration & dosage Caffeine - antagonists & inhibitors Cell physiology Degenerative and inherited degenerative diseases of the nervous system. Leukodystrophies. Prion diseases Equilibrative Nucleoside Transporter 1 - biosynthesis Equilibrative Nucleoside Transporter 1 - genetics Equilibrative Nucleoside Transporter 1 - physiology ethanol Ethanol - administration & dosage Fundamental and applied biological sciences. Psychology Gene expression Gene Expression Regulation - drug effects Gene Expression Regulation - physiology Humans Medical sciences Membrane and intracellular transports Mice Mice, Transgenic Molecular and cellular biology Neurology Neurons - drug effects Neurons - metabolism Neurons - physiology nucleoside transport PC12 Cells Rats Receptors, Purinergic P1 - biosynthesis Receptors, Purinergic P1 - genetics Rodents Transgenic animals Up-Regulation - genetics |
| Title | Transgenic expression of human equilibrative nucleoside transporter 1 in mouse neurons |
| URI | https://onlinelibrary.wiley.com/doi/abs/10.1111%2Fj.1471-4159.2009.05991.x https://www.ncbi.nlm.nih.gov/pubmed/19222701 https://www.proquest.com/docview/206552649 https://search.proquest.com/docview/20548102 https://search.proquest.com/docview/67096897 |
| Volume | 109 |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwrV1Lb9QwEB5BL3Dh0QINheID4pZVnPiRHKuFVVmJHoBFvVmOY6NVRRa6u1Lh1zPjPGCrIiHEJVplnVFmMvZ8tsffALxUGBXyUom0bKxLhSp4WlVCp5lrMPxlrpKazju_O1OnCzE_l-d9_hOdhen4IcYFN-oZcbymDm7r9bVOrnH-g_G4p52UiHUmhCd5oSm77_X7kUmKWLT4SByOjrqb1HOjoJ1IdTvYFeVN2jWaLnQ1L24CpbsYNwap2X24GNTrclMuJttNPXE_rjE__h_9H8C9Hsuyk875HsIt3-7DwUmL8_gv39krFrNL47L9PtyZDpXlDuBTDJDouUvH_FWfiduyVWCxYiDz37bL-G40FLOWGJdXVFWUbUYm9kvG2bJltG6BLYhgpF0_gsXszcfpadrXd0idRGCX1kEGXTsRrBR1I0pbe18WZXBlUeSlw7vCS9TRqqYJTVblGrFsrZQPQSFyqYvHsNeuWn8ITFvnpPWF1SiIi6ZElOIKyz0KdohgE-DDtzRfOxoP8_v0R3NDhqSinJWJhjRXCRziRzf2M462ZvEhpz1eouOTuUrgeMcTRpkIBIihP0_gaHAN0w8KaxSuJD4sqgRejP-i6WmLxrYeLYZNcAaJmO_PLYhvT5WVTuBJ53G_9KnoXHPGE1DRb_5aUTM_m9Kvp__64BHcHTbZMv4M9jaXW_8csdqmPo69EK-zt_Ofk4MvRg |
| link.rule.ids | 314,780,784,1375,27924,27925,46294,46718 |
| linkProvider | Wiley-Blackwell |
| linkToHtml | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwrV1Lb9QwEB5BOZQLjxZoKLQ-IG5ZxYntJMdqoVpKuwfoot4sx7HRCshCd1cq_HpmnAdsVSSEuEWJM4onM57P9vgbgBcKo0JaKBEXtbGxUBmPy1LkcWJrDH-JLWVO553PpmoyEycX8qIrB0RnYVp-iGHBjTwjjNfk4LQgfc3Lc5wAYUDueCclgp0RAso76P2c8rtevRu4pIhHiw_U4Wiqm2k9N0raiFW3vVlQ5qRZovJ8W_XiJli6iXJDmDq-D5_7DrbZKZ9G61U1sj-ucT_-Jw08gHsdnGVHrf09hFuu2YHdowan8l--s5csJJiGlfsd2B73xeV24UOIkWi8c8vcVZeM27CFZ6FoIHPf1vPwcTQas4ZIlxdUWJStBjL2S8bZvGG0dIEtiGOkWT6C2fHr8_Ek7ko8xFYitosrL31eWeGNFFUtClM5V2SFt0WWpYXFu8JJ7KNRde3rpExzhLOVUs57heClyh7DVrNo3B6w3FgrjctMjoK4qAsEKjYz3KFgiyA2At7_TP21ZfLQv8-Acq5JkVSXs9RBkfoqgj3869p8xAFXz96ntM1LjHwyVREcbJjCIBOxAJH0pxHs97ahu3FhicKVxJdFGcHh8BRVT7s0pnGoMWyCk0iEfX9uQZR7qijzCJ60JverPyUdbU54BCoYzl93VJ9Mx3T19F9fPITtyfnZqT59M327D3f7PbeEP4Ot1eXaPUfotqoOgkv-BHEMMm0 |
| linkToPdf | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwrV1Lb9QwEB5BkYALjxZoKLQ-IG5Z5WE7ybHasioFVghY1JvlODZaVXhLd1cq_HpmnAdsVSSEuEWJPYrHY89ne_wNwAuJXiErJY_LRpuYyzyNq4oXcWIadH-JqURB953fTeXxjJ-citMu_onuwrT8EMOGG42MMF_TAD9v3JVBXuD6B_1xRzspEOuMEE_e4jKriEf_6MNAJUU0WunAHI6WuhnVc62kDVd10-kFBU7qJerOtUkvrkOlmyA3eKnJfTjr29cGp5yN1qt6ZH5coX78Pwp4APc6MMsOW-t7CDes34adQ48L-a_f2UsWwkvDvv023Bn3qeV24HPwkGi6c8PsZReK69nCsZAykNlv63n4N5qLmSfK5QWlFWWrgYr9gqVs7hltXGAJYhjxy0cwm7z6ND6OuwQPsRGI7OLaCVfUhjsteN3wUtfWlnnpTJnnWWnwLbcC26hl07gmqbICwWwtpXVOInSp88ew5Rfe7gIrtDFC21wXKCjlTYkwxeQ6tSjYIISNIO37Up23PB7q9_VPkSpSJGXlrFRQpLqMYBc7XekvON2q2ceMDnmJj09kMoL9DUsYZCISIIr-LIK93jRUNyssUbgUWJlXERwMX1H1dEajvUWNYRFcQiLo-3MJItyTZVVE8KS1uF_tqehic5JGIIPd_HVD1cl0TE9P_7XiAdx-fzRRb19P3-zB3f7ALUmfwdbqYm2fI25b1fthQP4En7oxHA |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Transgenic+expression+of+human+equilibrative+nucleoside+transporter+1+in+mouse+neurons&rft.jtitle=Journal+of+neurochemistry&rft.au=Parkinson%2C+Fiona+E.&rft.au=Xiong%2C+Wei&rft.au=Zamzow%2C+Christina+R.&rft.au=Chestley%2C+Taeyo&rft.date=2009-04-01&rft.issn=0022-3042&rft.eissn=1471-4159&rft.volume=109&rft.issue=2&rft.spage=562&rft.epage=572&rft_id=info:doi/10.1111%2Fj.1471-4159.2009.05991.x&rft.externalDBID=n%2Fa&rft.externalDocID=10_1111_j_1471_4159_2009_05991_x |
| thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0022-3042&client=summon |
| thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0022-3042&client=summon |
| thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0022-3042&client=summon |