建兰新品种黄金小神童组培育苗集成技术的优化
为建兰新品种黄金小神童工厂化育苗利用提供技术参考,通过对外植体抗褐化技术、外植体诱导分化、原球茎继代增殖、从生芽生根诱导等最佳培养条件的筛选,对黄金小神童组培育苗集成技术进行了优化.结果表明：侧芽茎尖在消毒前先用2％维生素水溶液浸泡30 min后再用0.1％升汞常规消毒,能有效延缓外植体褐化时间和保持外植体活力,外植体褐化率在15％以下;1/2 MS＋1％琼脂＋1％蔗糖＋1 g/LAC＋1 g/L Na2SO3为筛选出的各阶段通用最佳基本培养基;最佳培养条件为温度25～28℃,光照时间14 h/d,光照强度30～40μmol/（m2·s）;最佳激素处理,初诱导为TDZ 3.0 mg/L ＋NA...
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| Published in | 西南农业学报 Vol. 27; no. 5; pp. 2135 - 2140 |
|---|---|
| Main Author | |
| Format | Journal Article |
| Language | Chinese |
| Published |
贵州省生物研究所 贵州贵阳550009
2014
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| Subjects | |
| Online Access | Get full text |
| ISSN | 1001-4829 |
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| Abstract | 为建兰新品种黄金小神童工厂化育苗利用提供技术参考,通过对外植体抗褐化技术、外植体诱导分化、原球茎继代增殖、从生芽生根诱导等最佳培养条件的筛选,对黄金小神童组培育苗集成技术进行了优化.结果表明：侧芽茎尖在消毒前先用2％维生素水溶液浸泡30 min后再用0.1％升汞常规消毒,能有效延缓外植体褐化时间和保持外植体活力,外植体褐化率在15％以下;1/2 MS＋1％琼脂＋1％蔗糖＋1 g/LAC＋1 g/L Na2SO3为筛选出的各阶段通用最佳基本培养基;最佳培养条件为温度25～28℃,光照时间14 h/d,光照强度30～40μmol/（m2·s）;最佳激素处理,初诱导为TDZ 3.0 mg/L ＋NAA 0.2 mg/L;继代增殖为TDZ3.5 mg/L＋NAA 0.16 mg/L;生根诱导为6-BA 0.3 mg,/L＋ NAA 1.2 mg/L.生根培养70～80d,大棚炼苗15～20d后出瓶移栽,用蛭石做基质覆盖水藓的移栽方法较单独用粉碎树皮或水藓的移栽效果好,移栽成活率达90％以上. |
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| AbstractList | 为建兰新品种黄金小神童工厂化育苗利用提供技术参考,通过对外植体抗褐化技术、外植体诱导分化、原球茎继代增殖、从生芽生根诱导等最佳培养条件的筛选,对黄金小神童组培育苗集成技术进行了优化.结果表明：侧芽茎尖在消毒前先用2％维生素水溶液浸泡30 min后再用0.1％升汞常规消毒,能有效延缓外植体褐化时间和保持外植体活力,外植体褐化率在15％以下;1/2 MS＋1％琼脂＋1％蔗糖＋1 g/LAC＋1 g/L Na2SO3为筛选出的各阶段通用最佳基本培养基;最佳培养条件为温度25～28℃,光照时间14 h/d,光照强度30～40μmol/（m2·s）;最佳激素处理,初诱导为TDZ 3.0 mg/L ＋NAA 0.2 mg/L;继代增殖为TDZ3.5 mg/L＋NAA 0.16 mg/L;生根诱导为6-BA 0.3 mg,/L＋ NAA 1.2 mg/L.生根培养70～80d,大棚炼苗15～20d后出瓶移栽,用蛭石做基质覆盖水藓的移栽方法较单独用粉碎树皮或水藓的移栽效果好,移栽成活率达90％以上. S682.31; 为建兰新品种黄金小神童工厂化育苗利用提供技术参考,通过对外植体抗褐化技术、外植体诱导分化、原球茎继代增殖、从生芽生根诱导等最佳培养条件的筛选,对黄金小神童组培育苗集成技术进行了优化.结果表明:侧芽茎尖在消毒前先用2％维生素水溶液浸泡30 min后再用0.1％升汞常规消毒,能有效延缓外植体褐化时间和保持外植体活力,外植体褐化率在15％以下;1/2 MS+1％琼脂+1％蔗糖+1 g/LAC+1 g/L Na2SO3为筛选出的各阶段通用最佳基本培养基;最佳培养条件为温度25～28℃,光照时间14 h/d,光照强度30～40μmol/(m2·s);最佳激素处理,初诱导为TDZ 3.0 mg/L +NAA 0.2 mg/L;继代增殖为TDZ3.5 mg/L+NAA 0.16 mg/L;生根诱导为6-BA 0.3 mg,/L+ NAA 1.2 mg/L.生根培养70～80d,大棚炼苗15～20d后出瓶移栽,用蛭石做基质覆盖水藓的移栽方法较单独用粉碎树皮或水藓的移栽效果好,移栽成活率达90％以上. |
| Author | 王济红 刘燕 祁翔 王莹 向立容 孙超 |
| AuthorAffiliation | 贵州省生物研究所,贵州贵阳550009 |
| AuthorAffiliation_xml | – name: 贵州省生物研究所 贵州贵阳550009 |
| Author_FL | QI Xiang XIANG Li-rong WANG Ji-hong LIU Yan SUN Chao WANG Ying |
| Author_FL_xml | – sequence: 1 fullname: WANG Ji-hong – sequence: 2 fullname: LIU Yan – sequence: 3 fullname: QI Xiang – sequence: 4 fullname: WANG Ying – sequence: 5 fullname: XIANG Li-rong – sequence: 6 fullname: SUN Chao |
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| ClassificationCodes | S682.31 |
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| Copyright | Copyright © Wanfang Data Co. Ltd. All Rights Reserved. |
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| DocumentTitleAlternate | Optimization of Integrated Technology for Tissue Culture of Cymbidium Golden Elf Sundust |
| DocumentTitle_FL | Optimization of Integrated Technology for Tissue Culture of Cymbidium Golden Elf Sundust |
| EndPage | 2140 |
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| GrantInformation_xml | – fundername: 贵州省农业攻关项目“几种名优建兰快繁育苗及栽培技术体系研究”(黔科合NY字; 贵州省体改项目基金“贵州省梵净山珍稀植物的保护与利用”(黔科合体z字 funderid: (2009)3044); (2010)4005) |
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| Issue | 5 |
| Keywords | 组织培养 黄金小神童 育苗 Raising plantlets 建兰新品种 关键技术 Tissue culture Cymbidium Golden Elf Sundust Key technology New Cymbidium ensifolium variety |
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| Notes | 51-1213/S The integrated technology for tissue culture of Cymbidium Golden Elf Sundust was optimized by screening optimum culture conditions for resistance to browning of explants,induced differentiation of explants,protocorm subculture multiplication and root induction of cluster buds to provide a technological reference for industrialized seedling production of Cymbidium Golden Elf Sundust.The results showed that：（i） The apexes of lateral buds soaked with 2 ％ Vc solution for 30min before regular disinfection of 0.1％ corrosive sublimate can effectively delay browning time of explants and keep explants vigor,and the explant browming rate is below 15 ％ ; （ii） The optimum basic medium for different culture stages is 1/2 MS ＋ 1％ agar ＋ 1％ sucrose ＋ 1 g/L AC ＋ 1 g/L Na2 SO3 ; （iii） The optimum culture condition is at 25-28 ℃ for 14 h/d lightng time under 30-40 pmol/（m2 · s） light intensity; （iv） The optimum hormone treatment for initial induclion,subculture multiplication and root induction is 3.0 mg/L TDZ ＋0.2 mg |
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| PublicationTitle | 西南农业学报 |
| PublicationTitleAlternate | Southwest China Journal of Agricultural Sciences |
| PublicationTitle_FL | Southwest China Journal of Agricultural Sciences |
| PublicationYear | 2014 |
| Publisher | 贵州省生物研究所 贵州贵阳550009 |
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| Snippet | 为建兰新品种黄金小神童工厂化育苗利用提供技术参考,通过对外植体抗褐化技术、外植体诱导分化、原球茎继代增殖、从生芽生根诱导等最佳培养条件的筛选,对黄金小神童组培育苗集成技术进行了优化.结果表明：侧芽茎尖在消毒前先用2％维生素水溶液浸泡30... S682.31; 为建兰新品种黄金小神童工厂化育苗利用提供技术参考,通过对外植体抗褐化技术、外植体诱导分化、原球茎继代增殖、从生芽生根诱导等最佳培养条件的筛选,对黄金小神童组培育苗集成技术进行了优化.结果表明:侧芽茎尖在消毒前先用2％维生素水溶液浸泡30... |
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| SubjectTerms | 关键技术 建兰新品种 组织培养 育苗 黄金小神童 |
| Title | 建兰新品种黄金小神童组培育苗集成技术的优化 |
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