Metatranscriptomics as a tool to identify fungal species and subspecies in mixed communities - a proof of concept under laboratory conditions

• Published in: IMA fungus Vol. 10; no. 1; p. 12
• Main Authors: Marcelino, Vanesa R, Irinyi, Laszlo, Eden, John-Sebastian, Meyer, Wieland, Holmes, Edward C, Sorrell, Tania C
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 08.08.2019; BioMed Central; BMC
• Subjects: Antifungal agents; Biodiversity; Contaminants; Cryptococcus; Deoxyribonucleic acid; DNA; DNA sequencing; Fungi; Genes; Genomes; Genomics; Laboratories; Meta-transcriptomics; Metagenomics; Microbiome; Next-generation sequencing; Nucleotide sequence; RNA; Species; Taxonomy; Virulence; Fungi; Metagenomics; Meta-transcriptomics; Microbiome

High-throughput sequencing (HTS) enables the generation of large amounts of genome sequence data at a reasonable cost. Organisms in mixed microbial communities can now be sequenced and identified in a culture-independent way, usually using amplicon sequencing of a DNA barcode. Bulk RNA-seq (metatran...