Identification of Glyoxalase-I as a Protein Marker in a Mouse Model of Extremes in Trait Anxiety

• Published in: The Journal of neuroscience Vol. 25; no. 17; pp. 4375 - 4384
• Main Authors: Kromer, Simone A, Kessler, Melanie S, Milfay, Dale, Birg, Isabel N, Bunck, Mirjam, Czibere, Ludwig, Panhuysen, Markus, Putz, Benno, Deussing, Jan M, Holsboer, Florian, Landgraf, Rainer, Turck, Christoph W
• Format: Journal Article
• Language: English
• Published: United States Soc Neuroscience 27.04.2005; Society for Neuroscience
• Subjects: Analysis of Variance; Animals; Animals, Newborn; Anti-Anxiety Agents - administration & dosage; Anxiety Disorders - diagnosis; Anxiety Disorders - drug therapy; Anxiety Disorders - enzymology; Anxiety Disorders - genetics; Avoidance Learning - drug effects; Avoidance Learning - physiology; Behavior, Animal; Behavioral/Systems/Cognitive; Biomarkers - metabolism; Blotting, Western - methods; Brain - drug effects; Brain - metabolism; Breeding - methods; Diazepam - administration & dosage; Disease Models, Animal; Electrophoresis, Gel, Two-Dimensional - methods; Exploratory Behavior - drug effects; Exploratory Behavior - physiology; Female; Hindlimb Suspension - physiology; Lactoylglutathione Lyase - isolation & purification; Lactoylglutathione Lyase - metabolism; Locomotion - genetics; Male; Mass Spectrometry - methods; Mice; Microarray Analysis - methods; Phenotype; Predictive Value of Tests; Proteomics - methods; Reaction Time - physiology; Reproducibility of Results; Sex Factors; Spatial Behavior - drug effects; Spatial Behavior - physiology; Statistics as Topic; Swimming; Time Factors; Vocalization, Animal - drug effects; Vocalization, Animal - physiology
• ISSN: 0270-6474; 1529-2401
• DOI: 10.1523/JNEUROSCI.0115-05.2005

For >15 generations, CD1 mice have been selectively and bidirectionally bred for either high-anxiety-related behavior (HAB-M) or low-anxiety-related behavior (LAB-M) on the elevated plus-maze. Independent of gender, HAB-M were more anxious than LAB-M animals in a variety of additional tests, including those reflecting risk assessment behaviors and ultrasound vocalization, with unselected CD1 "normal" control (NAB-M) and cross-mated (CM-M) mice displaying intermediate behavioral scores in most cases. Furthermore, in both the forced-swim and tail-suspension tests, LAB-M animals showed lower scores of immobility than did HAB-M and NAB-M animals, indicative of a reduced depression-like behavior. Using proteomic and microarray analyses, glyoxalase-I was identified as a protein marker, which is consistently expressed to a higher extent in LAB-M than in HAB-M mice in several brain areas. The same phenotype-dependent difference was found in red blood cells with NAB-M and CM-M animals showing intermediate expression profiles of glyoxalase-I. Additional studies will examine whether glyoxalase-I has an impact beyond that of a biomarker to predict the genetic predisposition to anxiety- and depression-like behavior.