Identification of two novel immunodominant UreB CD4+ T cell epitopes in Helicobacter pylori infected subjects
► UreB-specific CD4+ T cells from two H. pylori infected subjects were expanded in vitro. ► The UreB373–385 peptide recognized by UreB-specific CD4+ T cells from subject 4529 was restricted to DRB1*1404. ► The UreB438–452 peptide recognized by UreB-specific CD4+ T cells from subject 4493 is restrict...
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| Published in | Vaccine Vol. 31; no. 8; pp. 1204 - 1209 |
|---|---|
| Main Authors | , , , , , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
Kidlington
Elsevier Ltd
06.02.2013
Elsevier |
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| Online Access | Get full text |
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| Abstract | ► UreB-specific CD4+ T cells from two H. pylori infected subjects were expanded in vitro. ► The UreB373–385 peptide recognized by UreB-specific CD4+ T cells from subject 4529 was restricted to DRB1*1404. ► The UreB438–452 peptide recognized by UreB-specific CD4+ T cells from subject 4493 is restricted to DRB1*0803. ► The novel UreB epitopes are naturally processed and presented by antigen presenting cells.
An epitope-based vaccine is a promising option for treating Helicobacter pylori (H. pylori) infection. Epitope mapping is the first step in designing an epitope-based vaccine. A pivotal role of CD4+ T cells in protection against H. pylori has been accepted, but few Th epitopes have been identified. In this study, two novel UreB CD4+ T cell epitopes were identified using PBMCs obtained from two H. pylori infected subjects. We determined the restriction molecules by antibody blocking and used various Epstein–Barr virus-transformed B lymphocyte cell lines (BLCLs) with different HLA alleles as APCs to present peptides to CD4+ T cells. These epitopes were DRB1*1404-restricted UreB373–385 and DRB1*0803-restricted UreB438–452. The T cells specific to these epitopes not only recognized autologous DCs loaded with recombinant UreB but also those pulsed with H. pylori whole cell lysates, suggesting that these epitope peptides are naturally processed. These epitopes have important value for designing an effective H. pylori vaccine. |
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| AbstractList | An epitope-based vaccine is a promising option for treating Helicobacter pylori (H. pylori) infection. Epitope mapping is the first step in designing an epitope-based vaccine. A pivotal role of CD4+ T cells in protection against H. pylori has been accepted, but few Th epitopes have been identified. In this study, two novel UreB CD4+ T cell epitopes were identified using PBMCs obtained from two H. pylori infected subjects. We determined the restriction molecules by antibody blocking and used various Epstein–Barr virus-transformed B lymphocyte cell lines (BLCLs) with different HLA alleles as APCs to present peptides to CD4+ T cells. These epitopes were DRB1*1404-restricted UreB373–385 and DRB1*0803-restricted UreB438–452. The T cells specific to these epitopes not only recognized autologous DCs loaded with recombinant UreB but also those pulsed with H. pylori whole cell lysates, suggesting that these epitope peptides are naturally processed. These epitopes have important value for designing an effective H. pylori vaccine. ► UreB-specific CD4+ T cells from two H. pylori infected subjects were expanded in vitro. ► The UreB373–385 peptide recognized by UreB-specific CD4+ T cells from subject 4529 was restricted to DRB1*1404. ► The UreB438–452 peptide recognized by UreB-specific CD4+ T cells from subject 4493 is restricted to DRB1*0803. ► The novel UreB epitopes are naturally processed and presented by antigen presenting cells. An epitope-based vaccine is a promising option for treating Helicobacter pylori (H. pylori) infection. Epitope mapping is the first step in designing an epitope-based vaccine. A pivotal role of CD4+ T cells in protection against H. pylori has been accepted, but few Th epitopes have been identified. In this study, two novel UreB CD4+ T cell epitopes were identified using PBMCs obtained from two H. pylori infected subjects. We determined the restriction molecules by antibody blocking and used various Epstein–Barr virus-transformed B lymphocyte cell lines (BLCLs) with different HLA alleles as APCs to present peptides to CD4+ T cells. These epitopes were DRB1*1404-restricted UreB373–385 and DRB1*0803-restricted UreB438–452. The T cells specific to these epitopes not only recognized autologous DCs loaded with recombinant UreB but also those pulsed with H. pylori whole cell lysates, suggesting that these epitope peptides are naturally processed. These epitopes have important value for designing an effective H. pylori vaccine. Highlights ► UreB-specific CD4+ T cells from two H. pylori infected subjects were expanded in vitro. ► The UreB373–385 peptide recognized by UreB-specific CD4+ T cells from subject 4529 was restricted to DRB1*1404. ► The UreB438–452 peptide recognized by UreB-specific CD4+ T cells from subject 4493 is restricted to DRB1*0803. ► The novel UreB epitopes are naturally processed and presented by antigen presenting cells. An epitope-based vaccine is a promising option for treating Helicobacter pylori (H. pylori) infection. Epitope mapping is the first step in designing an epitope-based vaccine. A pivotal role of CD4⁺ T cells in protection against H. pylori has been accepted, but few Th epitopes have been identified. In this study, two novel UreB CD4⁺ T cell epitopes were identified using PBMCs obtained from two H. pylori infected subjects. We determined the restriction molecules by antibody blocking and used various Epstein–Barr virus-transformed B lymphocyte cell lines (BLCLs) with different HLA alleles as APCs to present peptides to CD4⁺ T cells. These epitopes were DRB1*1404-restricted UreB₃₇₃–₃₈₅ and DRB1*0803-restricted UreB₄₃₈–₄₅₂. The T cells specific to these epitopes not only recognized autologous DCs loaded with recombinant UreB but also those pulsed with H. pylori whole cell lysates, suggesting that these epitope peptides are naturally processed. These epitopes have important value for designing an effective H. pylori vaccine. An epitope-based vaccine is a promising option for treating Helicobacter pylori (H. pylori) infection. Epitope mapping is the first step in designing an epitope-based vaccine. A pivotal role of CD4(+) T cells in protection against H. pylori has been accepted, but few Th epitopes have been identified. In this study, two novel UreB CD4(+) T cell epitopes were identified using PBMCs obtained from two H. pylori infected subjects. We determined the restriction molecules by antibody blocking and used various Epstein-Barr virus-transformed B lymphocyte cell lines (BLCLs) with different HLA alleles as APCs to present peptides to CD4(+) T cells. These epitopes were DRB1*1404-restricted UreB(373-385) and DRB1*0803-restricted UreB(438-452). The T cells specific to these epitopes not only recognized autologous DCs loaded with recombinant UreB but also those pulsed with H. pylori whole cell lysates, suggesting that these epitope peptides are naturally processed. These epitopes have important value for designing an effective H. pylori vaccine. An epitope-based vaccine is a promising option for treating Helicobacter pylori (H. pylori) infection. Epitope mapping is the first step in designing an epitope-based vaccine. A pivotal role of CD4+ T cells in protection against H. pylori has been accepted, but few Th epitopes have been identified. In this study, two novel UreB CD4+ T cell epitopes were identified using PBMCs obtained from two H. pylori infected subjects. We determined the restriction molecules by antibody blocking and used various EpsteinaBarr virus-transformed B lymphocyte cell lines (BLCLs) with different HLA alleles as APCs to present peptides to CD4+ T cells. These epitopes were DRB1*1404-restricted UreB373a385 and DRB1*0803-restricted UreB438a452. The T cells specific to these epitopes not only recognized autologous DCs loaded with recombinant UreB but also those pulsed with H. pylori whole cell lysates, suggesting that these epitope peptides are naturally processed. These epitopes have important value for designing an effective H. pylori vaccine. An epitope-based vaccine is a promising option for treating Helicobacter pylori (H. pylori) infection. Epitope mapping is the first step in designing an epitope-based vaccine. A pivotal role of CD4(+) T cells in protection against H. pylori has been accepted, but few Th epitopes have been identified. In this study, two novel UreB CD4(+) T cell epitopes were identified using PBMCs obtained from two H. pylori infected subjects. We determined the restriction molecules by antibody blocking and used various Epstein-Barr virus-transformed B lymphocyte cell lines (BLCLs) with different HLA alleles as APCs to present peptides to CD4(+) T cells. These epitopes were DRB1*1404-restricted UreB(373-385) and DRB1*0803-restricted UreB(438-452). The T cells specific to these epitopes not only recognized autologous DCs loaded with recombinant UreB but also those pulsed with H. pylori whole cell lysates, suggesting that these epitope peptides are naturally processed. These epitopes have important value for designing an effective H. pylori vaccine.An epitope-based vaccine is a promising option for treating Helicobacter pylori (H. pylori) infection. Epitope mapping is the first step in designing an epitope-based vaccine. A pivotal role of CD4(+) T cells in protection against H. pylori has been accepted, but few Th epitopes have been identified. In this study, two novel UreB CD4(+) T cell epitopes were identified using PBMCs obtained from two H. pylori infected subjects. We determined the restriction molecules by antibody blocking and used various Epstein-Barr virus-transformed B lymphocyte cell lines (BLCLs) with different HLA alleles as APCs to present peptides to CD4(+) T cells. These epitopes were DRB1*1404-restricted UreB(373-385) and DRB1*0803-restricted UreB(438-452). The T cells specific to these epitopes not only recognized autologous DCs loaded with recombinant UreB but also those pulsed with H. pylori whole cell lysates, suggesting that these epitope peptides are naturally processed. These epitopes have important value for designing an effective H. pylori vaccine. |
| Author | Wu, Chao Li, Bin Hu, Jian Li, Hai-Bo Guo, Hong Zhang, Jin-Yong Zou, Quan-Ming Chen, Li Yang, Wu-Chen Yang, Shi-Ming |
| Author_xml | – sequence: 1 givenname: Wu-Chen surname: Yang fullname: Yang, Wu-Chen organization: Department of Clinical Microbiology and Immunology, College of Medical Laboratory Science, Third Military Medical University, Chongqing 400038, PR China – sequence: 2 givenname: Li surname: Chen fullname: Chen, Li organization: Department of Clinical Microbiology and Immunology, College of Medical Laboratory Science, Third Military Medical University, Chongqing 400038, PR China – sequence: 3 givenname: Hai-Bo surname: Li fullname: Li, Hai-Bo organization: Department of Clinical Microbiology and Immunology, College of Medical Laboratory Science, Third Military Medical University, Chongqing 400038, PR China – sequence: 4 givenname: Bin surname: Li fullname: Li, Bin organization: Department of Clinical Microbiology and Immunology, College of Medical Laboratory Science, Third Military Medical University, Chongqing 400038, PR China – sequence: 5 givenname: Jian surname: Hu fullname: Hu, Jian organization: Department of Gastroenterology, Xinqiao Hospital, Third Military Medical University, Chongqing 400038, PR China – sequence: 6 givenname: Jin-Yong surname: Zhang fullname: Zhang, Jin-Yong organization: Department of Clinical Microbiology and Immunology, College of Medical Laboratory Science, Third Military Medical University, Chongqing 400038, PR China – sequence: 7 givenname: Shi-Ming surname: Yang fullname: Yang, Shi-Ming organization: Department of Gastroenterology, Xinqiao Hospital, Third Military Medical University, Chongqing 400038, PR China – sequence: 8 givenname: Quan-Ming surname: Zou fullname: Zou, Quan-Ming organization: Department of Clinical Microbiology and Immunology, College of Medical Laboratory Science, Third Military Medical University, Chongqing 400038, PR China – sequence: 9 givenname: Hong surname: Guo fullname: Guo, Hong email: haojia2004@yahoo.com.cn organization: Department of Gastroenterology, Xinqiao Hospital, Third Military Medical University, Chongqing 400038, PR China – sequence: 10 givenname: Chao surname: Wu fullname: Wu, Chao email: wuchao99261@gmail.com organization: Department of Clinical Microbiology and Immunology, College of Medical Laboratory Science, Third Military Medical University, Chongqing 400038, PR China |
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| CitedBy_id | crossref_primary_10_1586_14760584_2015_1008460 crossref_primary_10_4161_gmic_27001 crossref_primary_10_1111_hel_13005 crossref_primary_10_1016_j_vaccine_2018_12_066 crossref_primary_10_1097_MOG_0b013e328365d443 crossref_primary_10_18632_oncotarget_11092 crossref_primary_10_3390_genes15030339 crossref_primary_10_1111_hel_12073 crossref_primary_10_3389_fcimb_2017_00349 crossref_primary_10_1016_j_humimm_2024_111212 crossref_primary_10_1093_femspd_ftv026 crossref_primary_10_1002_adfm_201802675 crossref_primary_10_1016_j_compbiolchem_2018_05_001 crossref_primary_10_1371_journal_pone_0083321 crossref_primary_10_1007_s10120_018_0867_1 crossref_primary_10_1016_j_vaccine_2018_07_033 crossref_primary_10_1111_imm_13703 crossref_primary_10_3389_fmicb_2018_00884 crossref_primary_10_1111_hel_12959 crossref_primary_10_1371_journal_pone_0094974 crossref_primary_10_3389_fcimb_2020_575271 crossref_primary_10_1016_j_vaccine_2024_02_050 |
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| Keywords | ICS FCM UreB Epitope HLA human leukocyte antigen intercellular cytokine staining flow cytometry Human HLA-System CD4 T lymphocyte Spirillales Antigenic determinant Spirillaceae Identification Immunodominance Helicobacter pylori Bacteria |
| Language | English |
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| Snippet | ► UreB-specific CD4+ T cells from two H. pylori infected subjects were expanded in vitro. ► The UreB373–385 peptide recognized by UreB-specific CD4+ T cells... Highlights ► UreB-specific CD4+ T cells from two H. pylori infected subjects were expanded in vitro. ► The UreB373–385 peptide recognized by UreB-specific CD4+... An epitope-based vaccine is a promising option for treating Helicobacter pylori (H. pylori) infection. Epitope mapping is the first step in designing an... |
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| SubjectTerms | alleles Allergy and Immunology Antigens, Bacterial - immunology Applied microbiology B-lymphocytes Bacteriology Biological and medical sciences CD4-positive T-lymphocytes CD4-Positive T-Lymphocytes - immunology Epitope Epitope Mapping epitopes Epitopes, T-Lymphocyte - immunology Fundamental and applied biological sciences. Psychology Helicobacter Infections - immunology Helicobacter pylori Helicobacter pylori - immunology HLA HLA-DRB1 Chains - immunology Humans Microbiology Miscellaneous peptides Urease - immunology UreB vaccines Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects) |
| Title | Identification of two novel immunodominant UreB CD4+ T cell epitopes in Helicobacter pylori infected subjects |
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