REHUNT: a reliable and open source package for restriction enzyme hunting

• Published in: BMC bioinformatics Vol. 19; no. 1; pp. 178 - 12
• Main Authors: Cheng, Yu-Huei, Liaw, Jiun-Jian, Kuo, Che-Nan
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 10.08.2018; BioMed Central; BMC
• Subjects: Algorithms; Bioinformatics; Biological analysis; Biotechnology; Cancer; Deoxyribonucleic acid; DNA; DNA methylation; DNA restriction enzymes; DNA Restriction Enzymes - genetics; Enzymes; Evaluation; Gene polymorphism; Gene sequencing; Genetic research; Genetics; Genomes; Genotypes; Genotyping; High-throughput screening; Humans; Hunting; Medical informatics; Methods; Mutation; Next-generation sequencing; Nucleotide sequence; Open source software; Plant breeding; Polymerase chain reaction; Polymorphism; Protein research; Researchers; Restriction fragment length polymorphism; Restriction Mapping - methods; Single-nucleotide polymorphism; Software; Software - standards; Studies

Restriction enzymes are used frequently in biotechnology. However, manual mining of restriction enzymes is challenging. Furthermore, integrating available restriction enzymes into different bioinformatics systems is necessary for many biotechnological applications, such as polymerase chain reaction-...