GREACE-assisted adaptive laboratory evolution in endpoint fermentation broth enhances lysine production by Escherichia coli

• Published in: Microbial cell factories Vol. 18; no. 1; pp. 106 - 13
• Main Authors: Wang, Xiaowei, Li, Qinggang, Sun, Cunmin, Cai, Zhen, Zheng, Xiaomei, Guo, Xuan, Ni, Xiaomeng, Zhou, Wenjuan, Guo, Yanmei, Zheng, Ping, Chen, Ning, Sun, Jibin, Li, Yin, Ma, Yanhe
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 11.06.2019; BioMed Central; BMC
• Subjects: Adaptive laboratory evolution; Amino acids; Arabinose; Backup software; Biological evolution; Chemicals; Chlorides; Deoxyribonucleic acid; DNA; DNA polymerase; DNA-directed DNA polymerase; E coli; Efficiency; Engineering; Escherichia coli; Evolution; Fermentation; Fermentation broth; Genetic aspects; Genetic research; Genomes; Genomics; Genotypes; Glucose; GREACE; Industrial engineering; Industrial production; Laboratories; Lysine; Lysine production; Manufacturing engineering; Metabolic flux; Metabolism; Metabolites; Metabolomics; Microorganisms; Molecular biology; Monosaccharides; Mutagenesis; Mutants; Mutation; Organic chemistry; Oxidative stress; Potassium; Production capacity; Proofreading; Proteins; Sodium chloride; Beijing China; United States > US; China; Lysine production; GREACE; Fermentation broth; Escherichia coli; Adaptive laboratory evolution

Late-stage fermentation broth contains high concentrations of target chemicals. Additionally, it contains various cellular metabolites which have leaked from lysed cells, which would exert multifactorial stress to industrial hyperproducers and perturb both cellular metabolism and product formation....