In vitro detection of in vitro secondary mechanisms of genotoxicity induced by engineered nanomaterials

• Published in: Particle and fibre toxicology Vol. 16; no. 1; pp. 8 - 14
• Main Authors: Evans, Stephen J, Clift, Martin J D, Singh, Neenu, Wills, John W, Hondow, Nicole, Wilkinson, Thomas S, Burgum, Michael J, Brown, Andy P, Jenkins, Gareth J, Doak, Shareen H
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 13.02.2019; BioMed Central; BMC
• Subjects: Biocompatibility; Biomedical materials; Bronchi - drug effects; Bronchi - immunology; Bronchi - pathology; Cell culture; Cell Differentiation - drug effects; Cell Differentiation - genetics; Cell division; Cell Survival - drug effects; Chromosome aberrations; Coculture Techniques; Composition; Conditioned media; Conditioning; Culture media; Culture Media, Conditioned; Cytokines - biosynthesis; Cytotoxicity; Damage assessment; Damage detection; Deoxyribonucleic acid; DNA; DNA Damage; Endocytosis - drug effects; Ferric Compounds - toxicity; Genotoxicity; Health aspects; Humans; Immune cells; Immune system; In vitro methods and tests; In vitro models; In Vitro Techniques; In vivo methods and tests; Iron oxides; Lungs; Macrophages; Macrophages - drug effects; Macrophages - immunology; Macrophages - pathology; Magnetite Nanoparticles - toxicity; Mathematical models; Methods; Monoculture; Mutagenesis; Mutagenicity Tests - methods; Nano(geno)toxicology; Nanomaterials; Nanoparticles; Nanotechnology; Oxidative stress; Predictions; Safety; Secondary genotoxicity; Studies; Testing; THP-1 Cells; Toxicity; Toxicity testing; Toxicology; United Kingdom; Nanoparticles; In vitro models; Conditioned media; Secondary genotoxicity; Nano(geno)toxicology; Immune cells; Co-culture models

It is well established that toxicological evaluation of engineered nanomaterials (NMs) is vital to ensure the health and safety of those exposed to them. Further, there is a distinct need for the development of advanced physiologically relevant in vitro techniques for NM hazard prediction due to the...