Comparing the normalization methods for the differential analysis of Illumina high-throughput RNA-Seq data

• Published in: BMC bioinformatics Vol. 16; no. 1; p. 347
• Main Authors: Li, Peipei, Piao, Yongjun, Shon, Ho Sun, Ryu, Keun Ho
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 28.10.2015; BioMed Central
• Subjects: Base Sequence; Comparative analysis; Correlation analysis; Databases, Genetic; Genes; Genome, Human; High-Throughput Nucleotide Sequencing; Humans; Methods; Polyadenylation; Real-Time Polymerase Chain Reaction; RNA - chemistry; RNA - genetics; Sequence Alignment; Sequence Analysis, RNA

Recently, rapid improvements in technology and decrease in sequencing costs have made RNA-Seq a widely used technique to quantify gene expression levels. Various normalization approaches have been proposed, owing to the importance of normalization in the analysis of RNA-Seq data. A comparison of rec...