Increased extracellular matrix deposition during chondrogenic differentiation of dental pulp stem cells from individuals with neurofibromatosis type 1: an in vitro 2D and 3D study

Neurofibromatosis 1 (NF1) presents a wide range of clinical manifestations, including bone alterations. Studies that seek to understand cellular and molecular mechanisms underlying NF1 orthopedic problems are of great importance to better understand the pathogenesis and the development of new therap...

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Published inOrphanet journal of rare diseases Vol. 13; no. 1; p. 98
Main Authors Almeida, Paula Nascimento, Barboza, Deuilton do Nascimento, Luna, Eloá Borges, Correia, Maria Clara de Macena, Dias, Rhayra Braga, Siquara de Sousa, Ana Caroline, Duarte, Maria Eugenia Leite, Rossi, Maria Isabel Doria, Cunha, Karin Soares
Format Journal Article
LanguageEnglish
Published England BioMed Central Ltd 25.06.2018
BioMed Central
BMC
Subjects
Adipogenesis - physiology
Adult
Analysis
Bone marrow
Care and treatment
Cartilage cells
Cell culture
Cell differentiation
Cell Differentiation - genetics
Cell Differentiation - physiology
Cells, Cultured
Chondrogenesis
Chondrogenesis - genetics
Chondrogenesis - physiology
Dental pulp
Dental Pulp - cytology
Development and progression
Extracellular matrix
Female
Genetic disorders
Health aspects
Humans
Male
Medical research
Metabolic pathways
Microscopy
Mutation
Neurofibromatosis
Neurofibromatosis 1
Neurofibromatosis 1 - metabolism
Neurological disorders
Pathogenesis
Physiological aspects
Rare diseases
Recklinghausen's disease
Stem cells
Stem Cells - cytology
Stem Cells - metabolism
Tumors
Young Adult
United States > US
Cell culture
Neurofibromatosis 1
Cell differentiation
Chondrogenesis
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Abstract Neurofibromatosis 1 (NF1) presents a wide range of clinical manifestations, including bone alterations. Studies that seek to understand cellular and molecular mechanisms underlying NF1 orthopedic problems are of great importance to better understand the pathogenesis and the development of new therapies. Dental pulp stem cells (DPSCs) are being used as an in vitro model for several diseases and appear as a suitable model for NF1. The aim of this study was to evaluate in vitro chondrogenic differentiation of DPSCs from individuals with NF1 using two-dimensional (2D) and three-dimensional (3D) cultures. To fulfill the criteria of the International Society for Cellular Therapy, DPSCs were characterized by surface antigen expression and by their multipotentiality, being induced to differentiate towards adipogenic, osteogenic, and chondrogenic lineages in 2D cultures. Both DPSCs from individuals with NF1 (NF1 DPSCs) and control cultures were positive for CD90, CD105, CD146 and negative for CD13, CD14, CD45 and CD271, and successfully differentiated after the protocols. Chondrogenic differentiation was evaluated in 2D and in 3D (pellet) cultures, which were further evaluated by optical microscopy and transmission electron microscopy (TEM). 2D cultures showed greater extracellular matrix deposition in NF1 DPSCs comparing with controls during chondrogenic differentiation. In semithin sections, control pellets hadhomogenous-sized intra and extracelullar matrix vesicles, whereas NF1 cultures had matrix vesicles of different sizes. TEM analysis showed higher amount of collagen fibers in NF1 cultures compared with control cultures. NF1 DPSCs presented increased extracellular matrix deposition during chondrogenic differentiation, which could be related to skeletal changes in individuals with NF1.
AbstractList Abstract Background Neurofibromatosis 1 (NF1) presents a wide range of clinical manifestations, including bone alterations. Studies that seek to understand cellular and molecular mechanisms underlying NF1 orthopedic problems are of great importance to better understand the pathogenesis and the development of new therapies. Dental pulp stem cells (DPSCs) are being used as an in vitro model for several diseases and appear as a suitable model for NF1. The aim of this study was to evaluate in vitro chondrogenic differentiation of DPSCs from individuals with NF1 using two-dimensional (2D) and three-dimensional (3D) cultures. Results To fulfill the criteria of the International Society for Cellular Therapy, DPSCs were characterized by surface antigen expression and by their multipotentiality, being induced to differentiate towards adipogenic, osteogenic, and chondrogenic lineages in 2D cultures. Both DPSCs from individuals with NF1 (NF1 DPSCs) and control cultures were positive for CD90, CD105, CD146 and negative for CD13, CD14, CD45 and CD271, and successfully differentiated after the protocols. Chondrogenic differentiation was evaluated in 2D and in 3D (pellet) cultures, which were further evaluated by optical microscopy and transmission electron microscopy (TEM). 2D cultures showed greater extracellular matrix deposition in NF1 DPSCs comparing with controls during chondrogenic differentiation. In semithin sections, control pellets hadhomogenous-sized intra and extracelullar matrix vesicles, whereas NF1 cultures had matrix vesicles of different sizes. TEM analysis showed higher amount of collagen fibers in NF1 cultures compared with control cultures. Conclusion NF1 DPSCs presented increased extracellular matrix deposition during chondrogenic differentiation, which could be related to skeletal changes in individuals with NF1.
Neurofibromin converts Ras from an active (Ras-GTP) to an inactive form (Ras-GDP) [2]. [...]mutations in the NF1 gene lead to an increased intracellular Ras-activity [3]. To consider stem cells from dental origin as a potential in vitro model for studying NF1, a better characterization of those cells is mandatory. Activated Ras continually activates numerous intracellular signaling pathways, such as p38 kinase and MAPK/ERK pathway [3]. [...]a possible explanation for the greater deposition of extracellular matrix found in our study after chondrogenic differentiation is the constant activation of Ras, which elevates p38 kinase and ERK levels during the entire process of chondrogenic differentiation. [...]the absence of Ki-67 expression demonstrated that the cells had achieved the terminal stage of differentiation.
Neurofibromatosis 1 (NF1) presents a wide range of clinical manifestations, including bone alterations. Studies that seek to understand cellular and molecular mechanisms underlying NF1 orthopedic problems are of great importance to better understand the pathogenesis and the development of new therapies. Dental pulp stem cells (DPSCs) are being used as an in vitro model for several diseases and appear as a suitable model for NF1. The aim of this study was to evaluate in vitro chondrogenic differentiation of DPSCs from individuals with NF1 using two-dimensional (2D) and three-dimensional (3D) cultures. To fulfill the criteria of the International Society for Cellular Therapy, DPSCs were characterized by surface antigen expression and by their multipotentiality, being induced to differentiate towards adipogenic, osteogenic, and chondrogenic lineages in 2D cultures. Both DPSCs from individuals with NF1 (NF1 DPSCs) and control cultures were positive for CD90, CD105, CD146 and negative for CD13, CD14, CD45 and CD271, and successfully differentiated after the protocols. Chondrogenic differentiation was evaluated in 2D and in 3D (pellet) cultures, which were further evaluated by optical microscopy and transmission electron microscopy (TEM). 2D cultures showed greater extracellular matrix deposition in NF1 DPSCs comparing with controls during chondrogenic differentiation. In semithin sections, control pellets hadhomogenous-sized intra and extracelullar matrix vesicles, whereas NF1 cultures had matrix vesicles of different sizes. TEM analysis showed higher amount of collagen fibers in NF1 cultures compared with control cultures. NF1 DPSCs presented increased extracellular matrix deposition during chondrogenic differentiation, which could be related to skeletal changes in individuals with NF1.
BACKGROUNDNeurofibromatosis 1 (NF1) presents a wide range of clinical manifestations, including bone alterations. Studies that seek to understand cellular and molecular mechanisms underlying NF1 orthopedic problems are of great importance to better understand the pathogenesis and the development of new therapies. Dental pulp stem cells (DPSCs) are being used as an in vitro model for several diseases and appear as a suitable model for NF1. The aim of this study was to evaluate in vitro chondrogenic differentiation of DPSCs from individuals with NF1 using two-dimensional (2D) and three-dimensional (3D) cultures.RESULTSTo fulfill the criteria of the International Society for Cellular Therapy, DPSCs were characterized by surface antigen expression and by their multipotentiality, being induced to differentiate towards adipogenic, osteogenic, and chondrogenic lineages in 2D cultures. Both DPSCs from individuals with NF1 (NF1 DPSCs) and control cultures were positive for CD90, CD105, CD146 and negative for CD13, CD14, CD45 and CD271, and successfully differentiated after the protocols. Chondrogenic differentiation was evaluated in 2D and in 3D (pellet) cultures, which were further evaluated by optical microscopy and transmission electron microscopy (TEM). 2D cultures showed greater extracellular matrix deposition in NF1 DPSCs comparing with controls during chondrogenic differentiation. In semithin sections, control pellets hadhomogenous-sized intra and extracelullar matrix vesicles, whereas NF1 cultures had matrix vesicles of different sizes. TEM analysis showed higher amount of collagen fibers in NF1 cultures compared with control cultures.CONCLUSIONNF1 DPSCs presented increased extracellular matrix deposition during chondrogenic differentiation, which could be related to skeletal changes in individuals with NF1.
ArticleNumber 98
Audience Academic
Author Correia, Maria Clara de Macena
Dias, Rhayra Braga
Siquara de Sousa, Ana Caroline
Almeida, Paula Nascimento
Duarte, Maria Eugenia Leite
Luna, Eloá Borges
Cunha, Karin Soares
Rossi, Maria Isabel Doria
Barboza, Deuilton do Nascimento
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  givenname: Maria Isabel Doria
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BackLink https://www.ncbi.nlm.nih.gov/pubmed/29941005$$D View this record in MEDLINE/PubMed
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Issue 1
Keywords Cell culture
Neurofibromatosis 1
Cell differentiation
Chondrogenesis
Language English
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Snippet Neurofibromatosis 1 (NF1) presents a wide range of clinical manifestations, including bone alterations. Studies that seek to understand cellular and molecular...
Neurofibromin converts Ras from an active (Ras-GTP) to an inactive form (Ras-GDP) [2]. [...]mutations in the NF1 gene lead to an increased intracellular...
BACKGROUNDNeurofibromatosis 1 (NF1) presents a wide range of clinical manifestations, including bone alterations. Studies that seek to understand cellular and...
Abstract Background Neurofibromatosis 1 (NF1) presents a wide range of clinical manifestations, including bone alterations. Studies that seek to understand...
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StartPage 98
SubjectTerms Adipogenesis - physiology
Adult
Analysis
Bone marrow
Care and treatment
Cartilage cells
Cell culture
Cell differentiation
Cell Differentiation - genetics
Cell Differentiation - physiology
Cells, Cultured
Chondrogenesis
Chondrogenesis - genetics
Chondrogenesis - physiology
Dental pulp
Dental Pulp - cytology
Development and progression
Extracellular matrix
Female
Genetic disorders
Health aspects
Humans
Male
Medical research
Metabolic pathways
Microscopy
Mutation
Neurofibromatosis
Neurofibromatosis 1
Neurofibromatosis 1 - metabolism
Neurological disorders
Pathogenesis
Physiological aspects
Rare diseases
Recklinghausen's disease
Stem cells
Stem Cells - cytology
Stem Cells - metabolism
Tumors
Young Adult
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Title Increased extracellular matrix deposition during chondrogenic differentiation of dental pulp stem cells from individuals with neurofibromatosis type 1: an in vitro 2D and 3D study
URI https://www.ncbi.nlm.nih.gov/pubmed/29941005
https://www.proquest.com/docview/2071806820
https://search.proquest.com/docview/2059562198
https://pubmed.ncbi.nlm.nih.gov/PMC6020206
https://doaj.org/article/7659ef5992364a859276ec6a3702ce63
Volume 13
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