Arabidopsis repressor of light signaling, COP1, is regulated by nuclear exclusion: mutational analysis by bioluminescence resonance energy transfer

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 101; no. 17; pp. 6798 - 6802
• Main Authors: Subramanian, C, Kim, B.H, Lyssenko, N.N, Xu, X, Johnson, C.H, Arnim, A.G. von
• Format: Journal Article
• Language: English
• Published: United States National Academy of Sciences 27.04.2004; National Acad Sciences
• Subjects: Alleles; Amino Acid Sequence; Analysis; Arabidopsis - metabolism; Arabidopsis - physiology; Arabidopsis Proteins - chemistry; Arabidopsis Proteins - genetics; Arabidopsis Proteins - metabolism; Arabidopsis Proteins - physiology; Arabidopsis thaliana; Biological Sciences; Bioluminescence; Botany; Cell Nucleus - metabolism; Dimerization; Energy; Energy Transfer; Epidermal cells; Flowers & plants; Fluorescence; Genetic Complementation Test; Hypocotyls; Light; Luminescent Measurements; Molecular Sequence Data; Mutant proteins; Mutation; Onions; Plant cells; Sequence Homology, Amino Acid; Signal Transduction - physiology; Transgenes

Bioluminescence resonance energy transfer (BRET) between Renilla luciferase and yellow fluorescent protein has been adapted to serve as a real-time reporter on protein-protein interactions in live plant cells by using the Arabidopsis Constitutive photomorphogenesis 1 (COP1) protein as a model system...