High-throughput sequencing reveals an altered T cell repertoire in X-linked agammaglobulinemia

• Published in: Clinical immunology (Orlando, Fla.) Vol. 161; no. 2; pp. 190 - 196
• Main Authors: Ramesh, Manish, Simchoni, Noa, Hamm, David, Cunningham-Rundles, Charlotte
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.12.2015
• Subjects: Adolescent; Adult; Agammaglobulinemia - genetics; Allergy and Immunology; Amino acid sequence; B-Lymphocytes - metabolism; Case-Control Studies; Child; Child, Preschool; DNA - genetics; Genetic Diseases, X-Linked - genetics; High throughput sequencing; High-Throughput Nucleotide Sequencing - methods; Humans; Immunoglobulin Variable Region - genetics; Infant; Junctional diversity; Male; Middle Aged; Receptor-CD3 Complex, Antigen, T-Cell - genetics; Receptors, Antigen, T-Cell - genetics; T cell receptor; T-Lymphocytes - metabolism; XLA; Young Adult; Amino acid sequence; Junctional diversity; T cell receptor; High throughput sequencing; XLA

To examine the T cell receptor structure in the absence of B cells, the TCR β CDR3 was sequenced from DNA of 15 X-linked agammaglobulinemia (XLA) subjects and 18 male controls, using the Illumina HiSeq platform and the ImmunoSEQ analyzer. V gene usage and the V–J combinations, derived from both prod...