油茶根际高效溶磷细菌的筛选、鉴定及其安全性测试

本研究利用溶磷圈法和钼锑抗比色法,从油茶根际分离出20株高效溶无机磷细菌,并对其溶磷能力进行定性和定量测试,结果发现,D/d与溶磷细菌的有效磷含量之间没有显著的相关性,而培养液pH与有效磷含量之间存在极显著的负相关性关系（P＜0.01）.并采用形态特征、生理生化、Biolog系统和16SrDNA序列分析对一株溶磷效果最好的菌株NC285进行菌株鉴定,确定其为Bacillus aryabhattai.通过苜蓿植物模型和cblA毒力基因测定对该菌株进行安全性检测,结果发现,该菌株未检测到cblA基因,对洋葱和苜蓿安全无致病性.本研究对溶磷细菌的安全应用及提高油茶土壤磷素利用效率具有重要意义....

Full description

Saved in:
Bibliographic Details
Published in林业科学研究 Vol. 28; no. 2; pp. 166 - 172
Main Author 王舒 张林平 郝菲菲 张扬 胡冬南
Format Journal Article
LanguageChinese
Published 江西农业大学园林与艺术学院,江西南昌,330045 2015
Subjects
安全性测试
油茶
溶磷细菌
溶磷能力
油茶
Camellia oleifera
phosphate-solubilizing bacteria
溶磷能力
phosphate-solubilizing capability
security test
溶磷细菌
安全性测试
Online AccessGet full text
ISSN1001-1498

Cover

Abstract 本研究利用溶磷圈法和钼锑抗比色法,从油茶根际分离出20株高效溶无机磷细菌,并对其溶磷能力进行定性和定量测试,结果发现,D/d与溶磷细菌的有效磷含量之间没有显著的相关性,而培养液pH与有效磷含量之间存在极显著的负相关性关系（P＜0.01）.并采用形态特征、生理生化、Biolog系统和16SrDNA序列分析对一株溶磷效果最好的菌株NC285进行菌株鉴定,确定其为Bacillus aryabhattai.通过苜蓿植物模型和cblA毒力基因测定对该菌株进行安全性检测,结果发现,该菌株未检测到cblA基因,对洋葱和苜蓿安全无致病性.本研究对溶磷细菌的安全应用及提高油茶土壤磷素利用效率具有重要意义.
AbstractList 本研究利用溶磷圈法和钼锑抗比色法,从油茶根际分离出20株高效溶无机磷细菌,并对其溶磷能力进行定性和定量测试,结果发现,D/d与溶磷细菌的有效磷含量之间没有显著的相关性,而培养液pH与有效磷含量之间存在极显著的负相关性关系（P＜0.01）.并采用形态特征、生理生化、Biolog系统和16SrDNA序列分析对一株溶磷效果最好的菌株NC285进行菌株鉴定,确定其为Bacillus aryabhattai.通过苜蓿植物模型和cblA毒力基因测定对该菌株进行安全性检测,结果发现,该菌株未检测到cblA基因,对洋葱和苜蓿安全无致病性.本研究对溶磷细菌的安全应用及提高油茶土壤磷素利用效率具有重要意义.
S794.4; 本研究利用溶磷圈法和钼锑抗比色法,从油茶根际分离出20株高效溶无机磷细菌,并对其溶磷能力进行定性和定量测试,结果发现,D/d与溶磷细菌的有效磷含量之间没有显著的相关性,而培养液pH与有效磷含量之间存在极显著的负相关性关系(P＜0.01).并采用形态特征、生理生化、Biolog系统和16SrDNA序列分析对一株溶磷效果最好的菌株NC285进行菌株鉴定,确定其为Bacillus aryabhattai.通过苜蓿植物模型和cblA毒力基因测定对该菌株进行安全性检测,结果发现,该菌株未检测到cblA基因,对洋葱和苜蓿安全无致病性.本研究对溶磷细菌的安全应用及提高油茶土壤磷素利用效率具有重要意义.
Author 王舒 张林平 郝菲菲 张扬 胡冬南
AuthorAffiliation 江西农业大学园林与艺术学院,江西南昌330045
AuthorAffiliation_xml – name: 江西农业大学园林与艺术学院,江西南昌,330045
Author_FL ZHANG Lin-ping
HU Dong-nan
HAO Fei-fei
ZHANG Yang
WANG Shu
Author_FL_xml – sequence: 1
  fullname: WANG Shu
– sequence: 2
  fullname: ZHANG Lin-ping
– sequence: 3
  fullname: HAO Fei-fei
– sequence: 4
  fullname: ZHANG Yang
– sequence: 5
  fullname: HU Dong-nan
Author_xml – sequence: 1
  fullname: 王舒 张林平 郝菲菲 张扬 胡冬南
BookMark eNotzj9Lw0AABfAbKthWv4SLU-D-5pJRSv0DBZfu4Xq5q6010QTRbhGs2FroVNC6CA4KYgXRSvXjSJLmWxio01t-vPdKoOD5niqAIoIQGYja1ioohWEbQswZRkVQTd7ni-EseZhnd73s5TYZXyffs_TxK_25WoyG6eQyfb3Pov5vdJH1P-LpJB4N4t4snvbj3nMSPSWfN4u38RpY0aITqvX_LIP6drVe2TVq-zt7la2aIZlNDVcJLTEmDegKZkPCNedcYQtZHFLdgMS0pEmUS_KbUhNTCqlswizkMlsrKkkZbC5rz4Snhdd02v5p4OWDTqd7eN5tY4gYxBDSXG4spTzwveZJK7fHQetIBF3HNCmjlCNM_gA8LGui
ClassificationCodes S794.4
ContentType Journal Article
Copyright Copyright © Wanfang Data Co. Ltd. All Rights Reserved.
Copyright_xml – notice: Copyright © Wanfang Data Co. Ltd. All Rights Reserved.
DBID 2RA
92L
CQIGP
W95
~WA
2B.
4A8
92I
93N
PSX
TCJ
DatabaseName 维普_期刊
中文科技期刊数据库-CALIS站点
中文科技期刊数据库-7.0平台
中文科技期刊数据库-农业科学
中文科技期刊数据库- 镜像站点
Wanfang Data Journals - Hong Kong
WANFANG Data Centre
Wanfang Data Journals
万方数据期刊 - 香港版
China Online Journals (COJ)
China Online Journals (COJ)
DatabaseTitleList

DeliveryMethod fulltext_linktorsrc
Discipline Forestry
DocumentTitleAlternate Screening, Identification and Security Test of Camellia oleifera Rhizosphere Phosphate-Solubilizing Bacteria
DocumentTitle_FL Screening, Identification and Security Test of Camellia oleifera Rhizosphere Phosphate-Solubilizing Bacteria
EndPage 172
ExternalDocumentID lykxyj201502004
664544712
GrantInformation_xml – fundername: 国家自然科学基金项目; 江西农业大学博士启动经费
  funderid: (31260194); (09004155)
GroupedDBID -04
2B.
2B~
2RA
3V.
5GY
5XA
5XE
7X2
92G
92I
92L
ABUWG
ACGFS
AFKRA
ALMA_UNASSIGNED_HOLDINGS
ATCPS
BENPR
BHPHI
BPHCQ
BVBZV
CCEZO
CCPQU
CHDYS
CQIGP
CW9
ECGQY
HCIFZ
M0K
PATMY
PQQKQ
PROAC
PYCSY
TCJ
TGD
U1G
U5N
W95
~WA
4A8
93N
AEUYN
PHGZM
PHGZT
PMFND
PSX
ID FETCH-LOGICAL-c594-deafc223b0da59037f777e2818704fb0368c63ed3498cf36cace93581d59fe4c3
ISSN 1001-1498
IngestDate Thu May 29 03:59:41 EDT 2025
Wed Feb 14 10:31:12 EST 2024
IsPeerReviewed false
IsScholarly true
Issue 2
Keywords 油茶
Camellia oleifera
phosphate-solubilizing bacteria
溶磷能力
phosphate-solubilizing capability
security test
溶磷细菌
安全性测试
Language Chinese
LinkModel OpenURL
MergedId FETCHMERGED-LOGICAL-c594-deafc223b0da59037f777e2818704fb0368c63ed3498cf36cace93581d59fe4c3
Notes 11-1221/S
WANG Shu , ZHANG Lin-ping, HAO Fei-fei, ZHANG Yang, HU Dong-nan （College of Landscape and Art,Jiangxi Agricultural University,Nanchang 330045 ,Jiangxi, China）
Twenty strains of inorganic phosphate-solubilizing bacteria were isolated from Camellia oleifera rhizosphere using phosphate-solubilizing circle and the molybdenum-anti-spectrophotometric method. The phosphate-solubilizing capacity was investigated from 20 strains based on qualitative and quantitative test. The results showed that there was a significant negative correlation between the phosphate solubilizing capacity and the pH medium（P 〈0. O1 ）, whereas there was no such phosphate solubilizing capacity on D/d. The strain NC285 was identified by its morpho- logical, physiological and biochemical characteristics, Biolog system and 16SrDNA gene sequence analysis. The high effective phosphate-solubilizing bacterium of NC285 was identified as Bacillus aryabhattai. The virulence of NC285 was tested on Allium cepa and Medicago sativa seedlings, and t
PageCount 7
ParticipantIDs wanfang_journals_lykxyj201502004
chongqing_primary_664544712
PublicationCentury 2000
PublicationDate 2015
PublicationDateYYYYMMDD 2015-01-01
PublicationDate_xml – year: 2015
  text: 2015
PublicationDecade 2010
PublicationTitle 林业科学研究
PublicationTitleAlternate Forest Research
PublicationTitle_FL Forest Research
PublicationYear 2015
Publisher 江西农业大学园林与艺术学院,江西南昌,330045
Publisher_xml – name: 江西农业大学园林与艺术学院,江西南昌,330045
SSID ssj0027521
ssib051373067
ssib001101254
ssib002263439
Score 2.0089846
Snippet ...
S794.4;...
SourceID wanfang
chongqing
SourceType Aggregation Database
Publisher
StartPage 166
SubjectTerms 安全性测试
油茶
溶磷细菌
溶磷能力
Title 油茶根际高效溶磷细菌的筛选、鉴定及其安全性测试
URI http://lib.cqvip.com/qk/96135X/201502/664544712.html
https://d.wanfangdata.com.cn/periodical/lykxyj201502004
Volume 28
hasFullText 1
inHoldings 1
isFullTextHit
isPrint
link http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnR1da9RAcDkqiC_iJ9aq3IP7dJwk2Wx293H3mlIEi0iFvh1JLmlRufrRgu1TBStWC30qaH0RfFAQK4ieVP-G_0DurvcvnNnkrlFE1JcwzM7Ozs5sNrNLZoaQixhL6cWC13mMSbXBha1HPHHqThTBaSyCA4GtGXllJpi-7l-e43OVyrfSX0vLS_GlZPW3cSX_Y1XAgV0xSvYfLDtiCgiAwb7wBAvD869sTMOAGo8aRUNJZYOaADHasRhFlaKSI6ANVRKbFKfSAkZbYkE1o0YgYAyVgeUzhawAozSVvqWZpMogH-lQCZyZBVyLUdQADac6RHoAsHsOcDuEbcJeFqPt6NBdCysGIA0OqqdoXgVz6ChbaYEnkPnUSMtcYC_lWp6TVOfyO1TlQoIko1tGRMiw4A0zVl4Ne5kG0h9w5qgow2p2JoyqyWL-oNMh8HM_mIdu1Gwjo9pKAkrTDQuAlkT5EiUPIC12fPynDI6JsvxJ8GRp6Xul_d0NgpKr4OZVh35J2B1gkjT45qNjAO6fHCOHTDhz9dqBl4o51cpZ7LyA-QfJWbnLBLNJ9Yf3BjwPHxxKimlAFhbb83fAubGxZu0sas-X3KLZY-RocZ6p6nxxHieV1YUT5DAWfMUqgidJ2Puwt7_Z6b3cGzxfH7x91tt-3PvS6b_63P_6aH9rs7_zsP_uxWBt4_vag8HGx-7uTnfrSXe9093d6K6_6a297n16uv9--xSZnQpnG9P1onRHPeHKr7fSKEvA8YydVsSVw0QmhEgx8Zhw_CwGr0kmAUtbDGaTZCxIoiS1mfhaXGWpn7DTZKy92E7PkCoWTPP9OJPKlX6SpeDQCs9reVwGcZK5_jiZGCmjeTvP0NIcmWCcVAv1NIv39l7z1srN-ys3cBU4uJmc_SODCXIEKfNLt3NkbOnucnoe3NCl-EJh1h_S22p1
linkProvider ProQuest
openUrl ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=%E6%B2%B9%E8%8C%B6%E6%A0%B9%E9%99%85%E9%AB%98%E6%95%88%E6%BA%B6%E7%A3%B7%E7%BB%86%E8%8F%8C%E7%9A%84%E7%AD%9B%E9%80%89%E3%80%81%E9%89%B4%E5%AE%9A%E5%8F%8A%E5%85%B6%E5%AE%89%E5%85%A8%E6%80%A7%E6%B5%8B%E8%AF%95&rft.jtitle=%E6%9E%97%E4%B8%9A%E7%A7%91%E5%AD%A6%E7%A0%94%E7%A9%B6&rft.au=%E7%8E%8B%E8%88%92+%E5%BC%A0%E6%9E%97%E5%B9%B3+%E9%83%9D%E8%8F%B2%E8%8F%B2+%E5%BC%A0%E6%89%AC+%E8%83%A1%E5%86%AC%E5%8D%97&rft.date=2015&rft.issn=1001-1498&rft.volume=28&rft.issue=2&rft.spage=166&rft.epage=172&rft.externalDocID=664544712
thumbnail_s http://utb.summon.serialssolutions.com/2.0.0/image/custom?url=http%3A%2F%2Fimage.cqvip.com%2Fvip1000%2Fqk%2F96135X%2F96135X.jpg
http://utb.summon.serialssolutions.com/2.0.0/image/custom?url=http%3A%2F%2Fwww.wanfangdata.com.cn%2Fimages%2FPeriodicalImages%2Flykxyj%2Flykxyj.jpg