Smad1参与调节雌激素在乳腺癌细胞中的促增殖作用
目的：观察在雌激素促进乳腺癌发生、发展的过程中,Smad1与雌激素的相互作用关系,以及其对乳腺癌细胞增殖、侵袭功能的影响,并探讨其作用机制。方法：通过Western印迹法以及实时定量聚合酶链式反应（real-time PCR）技术检测雌激素作用与Smad1表达的相关关系;采用小发卡RNA（short hairpin RNA,shRNA）干扰技术下调乳腺癌细胞中Smad1表达,并检测其对雌激素作用的影响。通过Transwell法、免疫组化法（IHC）、细胞计数法等研究Smad1表达情况对乳腺癌细胞增殖、侵袭能力的影响。结果：Smad1能够在雌激素刺激下提高其转录和翻译效应,雌激素作用4h后磷酸化...
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| Published in | 中国临床医学 Vol. 23; no. 2; pp. 144 - 148 |
|---|---|
| Main Author | |
| Format | Journal Article |
| Language | Chinese |
| Published |
南京医科大学第四临床医学院,南京,210029
2016
|
| Subjects | |
| Online Access | Get full text |
| ISSN | 1008-6358 |
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| Abstract | 目的：观察在雌激素促进乳腺癌发生、发展的过程中,Smad1与雌激素的相互作用关系,以及其对乳腺癌细胞增殖、侵袭功能的影响,并探讨其作用机制。方法：通过Western印迹法以及实时定量聚合酶链式反应（real-time PCR）技术检测雌激素作用与Smad1表达的相关关系;采用小发卡RNA（short hairpin RNA,shRNA）干扰技术下调乳腺癌细胞中Smad1表达,并检测其对雌激素作用的影响。通过Transwell法、免疫组化法（IHC）、细胞计数法等研究Smad1表达情况对乳腺癌细胞增殖、侵袭能力的影响。结果：Smad1能够在雌激素刺激下提高其转录和翻译效应,雌激素作用4h后磷酸化Smad-1（p-Smad1）被激活,并在12h达到最大值（P〈0.001）。乳腺癌肿瘤组织中Smad1的表达强于癌旁组织,且Smad1水平与雌激素受体α（ERα）正相关,而p-Smad1的活化被抑制。应用RNA干扰技术沉默两种乳腺癌细胞中Smad1后,细胞体外增殖速度显著下降（P〈0.05）;在体内试验中,肿瘤的生长也受到显著抑制（P〈0.05）。结论：雌激素对乳腺癌细胞的促增殖和迁移作用依赖于Smad1;骨形成蛋白（bone morphogenetic protein,BMP）-Smad1途径在雌激素促乳腺癌发生和发展过程中可能发挥重要的调节作用。 |
|---|---|
| AbstractList | R737.9; 目的:观察在雌激素促进乳腺癌发生、发展的过程中,Smad1与雌激素的相互作用关系,以及其对乳腺癌细胞增殖、侵袭功能的影响,并探讨其作用机制.方法:通过Western印迹法以及实时定量聚合酶链式反应(real-time PCR)技术检测雌激素作用与Smad1表达的相关关系;采用小发卡RNA(short hairpin RNA,shRNA)干扰技术下调乳腺癌细胞中Smad1表达,并检测其对雌激素作用的影响.通过Transwell法、免疫组化法(IHC)、细胞计数法等研究Smad1表达情况对乳腺癌细胞增殖、侵袭能力的影响.结果:Smad1能够在雌激素刺激下提高其转录和翻译效应,雌激素作用4h后磷酸化Smad-1(p-Smad1)被激活,并在12 h达到最大值(P＜0.001).乳腺癌肿瘤组织中Smad1的表达强于癌旁组织,且Smad1水平与雌激素受体α(ERα)正相关,而p-Smad1的活化被抑制.应用RNA干扰技术沉默两种乳腺癌细胞中Smad1后,细胞体外增殖速度显著下降(P＜0.05);在体内试验中,肿瘤的生长也受到显著抑制(P＜0.05).结论:雌激素对乳腺癌细胞的促增殖和迁移作用依赖于Smad1;骨形成蛋白(bone morphogenetic protein,BMP)-Smad1途径在雌激素促乳腺癌发生和发展过程中可能发挥重要的调节作用. 目的：观察在雌激素促进乳腺癌发生、发展的过程中,Smad1与雌激素的相互作用关系,以及其对乳腺癌细胞增殖、侵袭功能的影响,并探讨其作用机制。方法：通过Western印迹法以及实时定量聚合酶链式反应（real-time PCR）技术检测雌激素作用与Smad1表达的相关关系;采用小发卡RNA（short hairpin RNA,shRNA）干扰技术下调乳腺癌细胞中Smad1表达,并检测其对雌激素作用的影响。通过Transwell法、免疫组化法（IHC）、细胞计数法等研究Smad1表达情况对乳腺癌细胞增殖、侵袭能力的影响。结果：Smad1能够在雌激素刺激下提高其转录和翻译效应,雌激素作用4h后磷酸化Smad-1（p-Smad1）被激活,并在12h达到最大值（P〈0.001）。乳腺癌肿瘤组织中Smad1的表达强于癌旁组织,且Smad1水平与雌激素受体α（ERα）正相关,而p-Smad1的活化被抑制。应用RNA干扰技术沉默两种乳腺癌细胞中Smad1后,细胞体外增殖速度显著下降（P〈0.05）;在体内试验中,肿瘤的生长也受到显著抑制（P〈0.05）。结论：雌激素对乳腺癌细胞的促增殖和迁移作用依赖于Smad1;骨形成蛋白（bone morphogenetic protein,BMP）-Smad1途径在雌激素促乳腺癌发生和发展过程中可能发挥重要的调节作用。 |
| Author | 王景 张鹤达 李建 赵建华 吴建中 唐金海 |
| AuthorAffiliation | 南京医科大学第四临床医学院,南京210029 |
| AuthorAffiliation_xml | – name: 南京医科大学第四临床医学院,南京,210029 |
| Author_FL | ZHAO Jian-hua TANG Jin-hai ZHANG He-da WU Jian-zhong LI Jian WANG Jing |
| Author_FL_xml | – sequence: 1 fullname: WANG Jing – sequence: 2 fullname: ZHANG He-da – sequence: 3 fullname: LI Jian – sequence: 4 fullname: ZHAO Jian-hua – sequence: 5 fullname: WU Jian-zhong – sequence: 6 fullname: TANG Jin-hai |
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| ClassificationCodes | R737.9 |
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| Copyright | Copyright © Wanfang Data Co. Ltd. All Rights Reserved. |
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| DocumentTitleAlternate | Smadl involves in regulating proliferation effect of estrogen in breast cancer cells |
| DocumentTitle_FL | Smad1 involves in regulating proliferation effect of estrogen in breast cancer cells |
| EndPage | 148 |
| ExternalDocumentID | zglcyx201602004 669227801 |
| GrantInformation_xml | – fundername: 国家高技术研究发展资助项目; 国家自然科学基金; 江苏省科技计划面上项目(201513279).Supported by "863" project; National Natural Science Foundation of China; Science Project of Jiangsu Province funderid: (“863”项目,2014AA020604); (81272470); (2014AA020604); (81272470); (201513279) |
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| Keywords | Smad1 breast cancer 乳腺癌 雌激素受体 ER |
| Language | Chinese |
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| Notes | 31-1794/R Smadl; breast cancer; ER Objective： To investigate the interaction between Smadl and estrogen in the promotion of breast cancer, as well as its functional role and underlying mechanism. Methods： The interaction relationship of Smadl and estrogen was detected by Western blotting and real-time PCR. Smadl was down-regulated using short hairpin RNA （shRNA） interference technology in breast cancer cells. Using Transwell method, IHC and cell counting method to observe the effect of Smadl on cell proliferation and invasion. Results： Smadl could increase transcription and translation of breast cancer cells in the stimulation of estrogen, p-Smadl was activated after four hour activation of estrogen and then reach maximum at twelve hour later （P〈 0. 001）. The expression of Smadl in cancer tissue was enhanced compared to paratumor tissues and Smadl level was positively correlated with ERa while p-Smadl activation was inhibited. In vitro cell proliferation, RNA interference silencing of Smadl in two breast cancer |
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| PublicationTitle | 中国临床医学 |
| PublicationTitleAlternate | Chinese Journal Of Clinical Medicine |
| PublicationTitle_FL | Chinese Journal of Clinical Medicine |
| PublicationYear | 2016 |
| Publisher | 南京医科大学第四临床医学院,南京,210029 |
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| Snippet | 目的：观察在雌激素促进乳腺癌发生、发展的过程中,Smad1与雌激素的相互作用关系,以及其对乳腺癌细胞增殖、侵袭功能的影响,并探讨其作用机制。方法：通过Western印迹法以及实时定量聚合酶链式反应（real-time PCR）技术检测雌激素作用与Smad1表达的相关关系;采用小发卡RNA（short hairpin... R737.9; 目的:观察在雌激素促进乳腺癌发生、发展的过程中,Smad1与雌激素的相互作用关系,以及其对乳腺癌细胞增殖、侵袭功能的影响,并探讨其作用机制.方法:通过Western印迹法以及实时定量聚合酶链式反应(real-time PCR)技术检测雌激素作用与Smad1表达的相关关系;采用小发卡RNA(short... |
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| StartPage | 144 |
| SubjectTerms | Smad1 乳腺癌 雌激素受体 |
| Title | Smad1参与调节雌激素在乳腺癌细胞中的促增殖作用 |
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