胰岛素对子宫内膜癌细胞Shc/ERK通路活化的影响
背景与目的探讨胰岛素对子宫内膜癌细胞Src同源区2结构域蛋白C（Shc）活化、Shc与生长因子受体结合蛋白2（Grb2）的结合水平及对细胞外信号调节激酶（ERK）活化程度的影响。方法10-6mol/L胰岛素作用子宫内膜癌Ishikawa3-H^-12细胞不同时间,检测不同时间点细胞Shc磷酸化、Shc·Grb2复合物水平以及ERK的磷酸化程度。结果胰岛素以时间依赖方式活化导子宫内膜癌细胞Shc/ERK通路,Shc在胰岛素作用1min即出现活化增强,15min时达到峰值[p-p52Shc/p52Shc（73.75±2.93vs16.89±1.92）;p-p46Shc/p46Shc（35.65±5...
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| Published in | Zhongguo fei ai za zhi Vol. 12; no. 6; pp. 724 - 727 |
|---|---|
| Main Author | |
| Format | Journal Article |
| Language | Chinese |
| Published |
天津医科大学总医院,天津,300052
2009
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| Subjects | |
| Online Access | Get full text |
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| Abstract | 背景与目的探讨胰岛素对子宫内膜癌细胞Src同源区2结构域蛋白C（Shc）活化、Shc与生长因子受体结合蛋白2（Grb2）的结合水平及对细胞外信号调节激酶（ERK）活化程度的影响。方法10-6mol/L胰岛素作用子宫内膜癌Ishikawa3-H^-12细胞不同时间,检测不同时间点细胞Shc磷酸化、Shc·Grb2复合物水平以及ERK的磷酸化程度。结果胰岛素以时间依赖方式活化导子宫内膜癌细胞Shc/ERK通路,Shc在胰岛素作用1min即出现活化增强,15min时达到峰值[p-p52Shc/p52Shc（73.75±2.93vs16.89±1.92）;p-p46Shc/p46Shc（35.65±5.17vs13.71±2.77）]。细胞静息状态下检测不到Shc·Grb2复合物,胰岛素能促进Shc·Grb2结合,与Shc活化时程一致,即1min可检测出Shc·Grb2结合,15min时结合程度最大。胰岛素显著诱导子宫内膜癌细胞ERK活化,具有时间依赖性,作用30min时达到峰值（p-ERK/ERK：63.40±4.52vs22.66±3.76）。结论胰岛素以时间依赖模式促进子宫内膜癌Ishikawa3-H^-12细胞Shc活化、Shc·Grb2结合以及ERK的活化。 |
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| AbstractList | R734.2; 背景与目的 探讨胰岛素对子宫内膜癌细胞src同源区结构域蛋白C(Shc)活化、Shc与生长因子受体结合蛋白2(Grb2)的结合水平及对细胞外信号调节激酶(ERK)活化程度的影响.方法 106mol/L胰岛素作用子宫内膜癌Ishikawa3-H-12细胞不同时间,检测不同时间点细胞Shc磷酸化、Shc·Grb2复合物水平以及ERK的磷酸化程度.结果 胰岛素以时间依赖方式活化导子宫内膜癌细胞shc/ERK通路,Shc在胰岛素作用1 min即出现活化增强,15 min时达到峰值[P-p52Shc/p52Shc(73.75±2.93 vs 16.89±1.92);p-p46shc/p46Shc(35.65±5.17 vs 13.71±2.77)].细胞静息状态下检测不到Shc·Grb2复合物,胰岛素能促进Shc·Grb2结合,与Shc活化时程一致,即1min可检测出Shc·Grb2结合,15min时结合程度最大.胰岛素显著诱导子宫内膜癌细胞ERK活化,具有时问依赖性,作用30min时达到峰值(p-ERK/ERK:63.40±4.52 vs 22.66±3.76).结论 胰岛素以时间依赖模式促进子宫内膜癌Ishikawa3-H-12细胞Shc活化、Shc·Grb2结合以及ERK的活化. 背景与目的探讨胰岛素对子宫内膜癌细胞Src同源区2结构域蛋白C（Shc）活化、Shc与生长因子受体结合蛋白2（Grb2）的结合水平及对细胞外信号调节激酶（ERK）活化程度的影响。方法10-6mol/L胰岛素作用子宫内膜癌Ishikawa3-H^-12细胞不同时间,检测不同时间点细胞Shc磷酸化、Shc·Grb2复合物水平以及ERK的磷酸化程度。结果胰岛素以时间依赖方式活化导子宫内膜癌细胞Shc/ERK通路,Shc在胰岛素作用1min即出现活化增强,15min时达到峰值[p-p52Shc/p52Shc（73.75±2.93vs16.89±1.92）;p-p46Shc/p46Shc（35.65±5.17vs13.71±2.77）]。细胞静息状态下检测不到Shc·Grb2复合物,胰岛素能促进Shc·Grb2结合,与Shc活化时程一致,即1min可检测出Shc·Grb2结合,15min时结合程度最大。胰岛素显著诱导子宫内膜癌细胞ERK活化,具有时间依赖性,作用30min时达到峰值（p-ERK/ERK：63.40±4.52vs22.66±3.76）。结论胰岛素以时间依赖模式促进子宫内膜癌Ishikawa3-H^-12细胞Shc活化、Shc·Grb2结合以及ERK的活化。 |
| Author | 张丽志 薛凤霞 王颖梅 华绍芳 赵敬 |
| AuthorAffiliation | 天津医科大学总医院,天津300052 |
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| Keywords | 生长因子受体结合蛋白2 胰岛素 src同源区2结构域蛋白C 子宫内膜癌 细胞外信号调节激酶 |
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| SubjectTerms | Src同源区2结构域蛋白C 子宫内膜癌 生长因子受体结合蛋白2 细胞外信号调节激酶 胰岛素 |
| Title | 胰岛素对子宫内膜癌细胞Shc/ERK通路活化的影响 |
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