小麦1BL／1RS易位系的生化和分子标记鉴定
本文利用A-PAGE方法对87份供试的小麦材料进行了黑麦碱（secalin）检测，并进一步利用低分子量麦谷蛋白Glu—B3的STS—PCR标记以及与抗条锈病基因Yr9紧密连锁的Xgwm582标记对上述材料进行了1BL／1RS易位检测。结果表明，在87份材料中鉴定出了一种新的改良1BL／1RS易位系，该类易位系的特征是：含有来自1RS的抗条锈病基因Yr9、不含黑麦碱、具有Glu-B3基因、比一般1BL／IRS易位系具有明显偏高的SDS-沉降值。本研究中选用的生化标记和分子标记可以有效检测出育种材料中的1BL／1RS、非1BL／1RS以及改良1BL／1RS易位系。...
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| Published in | Xinan nongye xuebao Vol. 20; no. 1; pp. 15 - 18 |
|---|---|
| Main Author | |
| Format | Journal Article |
| Language | Chinese |
| Published |
中国科学院成都生物研究所,四川,成都,610041
2007
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| Subjects | |
| Online Access | Get full text |
| ISSN | 1001-4829 |
| DOI | 10.3969/j.issn.1001-4829.2007.01.004 |
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| Abstract | 本文利用A-PAGE方法对87份供试的小麦材料进行了黑麦碱（secalin）检测，并进一步利用低分子量麦谷蛋白Glu—B3的STS—PCR标记以及与抗条锈病基因Yr9紧密连锁的Xgwm582标记对上述材料进行了1BL／1RS易位检测。结果表明，在87份材料中鉴定出了一种新的改良1BL／1RS易位系，该类易位系的特征是：含有来自1RS的抗条锈病基因Yr9、不含黑麦碱、具有Glu-B3基因、比一般1BL／IRS易位系具有明显偏高的SDS-沉降值。本研究中选用的生化标记和分子标记可以有效检测出育种材料中的1BL／1RS、非1BL／1RS以及改良1BL／1RS易位系。 |
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| AbstractList | S512.035.3; 本文利用A-PAGE方法对87份供试的小麦材料进行了黑麦碱(seealin)检测,并进一步利用低分子量麦谷蛋白Glu-B3的STS-PCR标记以及与抗条锈病基因Yr9紧密连锁的Xgwm582标记对上述材料进行了1BL/1RS易位检测.结果表明,在87份材料中鉴定出了一种新的改良1BL/1RS易位系,该类易位系的特征是:含有来自1RS的抗条锈病基因Yr9、不含黑麦碱、具有Glu-B3基因、比一般1BL/IRS易位系具有明显偏高的SDS-沉降值.本研究中选用的生化标记和分子标记可以有效检测出育种材料中的1BL/1RS、非1BL/1RS以及改良1BL/1RS易位系. 本文利用A-PAGE方法对87份供试的小麦材料进行了黑麦碱（secalin）检测，并进一步利用低分子量麦谷蛋白Glu—B3的STS—PCR标记以及与抗条锈病基因Yr9紧密连锁的Xgwm582标记对上述材料进行了1BL／1RS易位检测。结果表明，在87份材料中鉴定出了一种新的改良1BL／1RS易位系，该类易位系的特征是：含有来自1RS的抗条锈病基因Yr9、不含黑麦碱、具有Glu-B3基因、比一般1BL／IRS易位系具有明显偏高的SDS-沉降值。本研究中选用的生化标记和分子标记可以有效检测出育种材料中的1BL／1RS、非1BL／1RS以及改良1BL／1RS易位系。 |
| Author | 吴崇明 徐智斌 王涛 |
| AuthorAffiliation | 中国科学院成都生物研究所,四川成都610041 |
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| Title | 小麦1BL／1RS易位系的生化和分子标记鉴定 |
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