Reduced DNA methylation in Arabidopsis thaliana results in abnormal plant development

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 93; no. 16; pp. 8449 - 8454
• Main Authors: Finnegan, E.J. (Commonwealth Scientific and Industrial Research Organization, Canberra, ACT, Australia.), Peacock, W.J, Dennis, E.S
• Format: Journal Article
• Language: English
• Published: United States National Academy of Sciences of the United States of America 06.08.1996; National Acad Sciences; National Academy of Sciences
• Subjects: 5-Methylcytosine; abnormal development; ADN; analogs & derivatives; ANATOMIA DE LA PLANTA; ANATOMIE VEGETALE; antisense DNA; apical dominance; Arabidopsis; Arabidopsis - genetics; Arabidopsis - growth & development; ARABIDOPSIS THALIANA; Botany; complementary DNA; Cytosine; Cytosine - analogs & derivatives; Cytosine - metabolism; Deoxyribonucleic acid; DNA; DNA (Cytosine-5-)-Methyltransferases; DNA (Cytosine-5-)-Methyltransferases - genetics; DNA (Cytosine-5-)-Methyltransferases - metabolism; DNA - metabolism; DNA methylation; DNA, Antisense; DNA-binding proteins; Enzymes; ETAPAS DE DESARROLLO DE LA PLANTA; EXPRESION GENICA; EXPRESSION DES GENES; FERTILIDAD; FERTILITE; FLORACION; FLORAISON; Flowers; Flowers & plants; GENE; Gene expression; Gene Expression Regulation, Developmental; GENES; genetics; growth & development; homeotic genes; INHIBICION; INHIBITION; Leaves; metabolism; Methylation; Mutation; Petals; Phenotype; Phenotypes; plant development; Plant growth; plant morphology; Plant reproduction; PLANTAS TRANSGENICAS; PLANTE TRANSGENIQUE; Plants; Plants, Genetically Modified; PROTEINAS AGLUTINANTES; PROTEINE DE LIAISON; RAMIFICACION; RAMIFICATION; RNA, Messenger; RNA, Messenger - genetics; RNA, Plant; RNA, Plant - genetics; STADE DE DEVELOPPEMENT VEGETAL; structural genes; TEMPS; TIEMPO; transcription factors; TRANSFERASAS; TRANSFERASE; transferases; Transgenes; transgenic plants; TRASTORNOS DEL CRECIMIENTO; TROUBLE DE LA CROISSANCE
• ISSN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.93.16.8449

Arabidopsis plants transformed with an antisense construct of an Arabidopsis methyltransferase cDNA (METI) have reduced cytosine methylation in CG dinucleotides. Methylation levels in progeny of five independent transformants ranged from 10% to 100% of the wild type. Removal of the antisense constru...