Reduced DNA methylation in Arabidopsis thaliana results in abnormal plant development
Arabidopsis plants transformed with an antisense construct of an Arabidopsis methyltransferase cDNA (METI) have reduced cytosine methylation in CG dinucleotides. Methylation levels in progeny of five independent transformants ranged from 10% to 100% of the wild type. Removal of the antisense constru...
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| Published in | Proceedings of the National Academy of Sciences - PNAS Vol. 93; no. 16; pp. 8449 - 8454 |
|---|---|
| Main Authors | , , |
| Format | Journal Article |
| Language | English |
| Published |
United States
National Academy of Sciences of the United States of America
06.08.1996
National Acad Sciences National Academy of Sciences |
| Subjects | |
| Online Access | Get full text |
| ISSN | 0027-8424 1091-6490 |
| DOI | 10.1073/pnas.93.16.8449 |
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| Abstract | Arabidopsis plants transformed with an antisense construct of an Arabidopsis methyltransferase cDNA (METI) have reduced cytosine methylation in CG dinucleotides. Methylation levels in progeny of five independent transformants ranged from 10% to 100% of the wild type. Removal of the antisense construct by segregation in sexual crosses did not fully restore methylation patterns in the progeny, indicating that methylation patterns are subject to meiotic inheritance in Arabidopsis. Plants with decreased methylation displayed a number of phenotypic and developmental abnormalities, including reduced apical dominance, smaller plant size, altered leaf size and shape, decreased fertility, and altered flowering time. Floral organs showed homeotic transformations that were associated with ectopic expression of the floral homeotic genes AGAMOUS and APETALA3 in leaf tissue. These observations suggest that DNA methylation plays an important role in regulating many developmental pathways in plants and that the developmental abnormalities seen in the methyltransferase antisense plants may be due to dysregulation of gene expression. |
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| AbstractList | Results of a study suggest that DNA methylation plays an important role in regulating many developmental pathways in plants, and that the developmental abnormalities seen in the study's methyltransferase antisense Arabidopsis plants may be due to dysregulation of gene expression. Arabidopsis plants transformed with an antisense construct of an Arabidopsis methyltransferase cDNA (METI) have reduced cytosine methylation in CG dinucleotides. Methylation levels in progeny of five independent transformants ranged from 10% to 100% of the wild type. Removal of the antisense construct by segregation in sexual crosses did not fully restore methylation patterns in the progeny, indicating that methylation patterns are subject to meiotic inheritance in Arabidopsis. Plants with decreased methylation displayed a number of phenotypic and developmental abnormalities, including reduced apical dominance, smaller plant size, altered leaf size and shape, decreased fertility, and altered flowering time. Floral organs showed homeotic transformations that were associated with ectopic expression of the floral homeotic genes AGAMOUS and APETALA3 in leaf tissue. These observations suggest that DNA methylation plays an important role in regulating many developmental pathways in plants and that the developmental abnormalities seen in the methyltransferase antisense plants may be due to dysregulation of gene expression. Arabidopsis plants transformed with an antisense construct of an Arabidopsis methyltransferase cDNA (METI) have reduced cytosine methylation in CG dinucleotides. Methylation levels in progeny of five independent transformants ranged from 10% to 100% of the wild type. Removal of the antisense construct by segregation in sexual crosses did not fully restore methylation patterns in the progeny, indicating that methylation patterns are subject to meiotic inheritance in Arabidopsis. Plants with decreased methylation displayed a number of phenotypic and developmental abnormalities, including reduced apical dominance, smaller plant size, altered leaf size and shape, decreased fertility, and altered powering time. Floral organs showed homeotic transformations that were associated with ectopic expression of the floral homeotic genes AGAMOUS and APETALA3 in leaf tissue. These observations suggest that DNA methylation plays an important role in regulating many developmental pathways in plants and that the developmental abnormalities seen in the methyltransferase antisense plants may be due to dysregulation of gene expression Arabidopsis plants transformed with an antisense construct of an Arabidopsis methyltransferase cDNA (METI) have reduced cytosine methylation in CG dinucleotides. Methylation levels in progeny of five independent transformants ranged from 10% to 100% of the wild type. Removal of the antisense construct by segregation in sexual crosses did not fully restore methylation patterns in the progeny, indicating that methylation patterns are subject to meiotic inheritance in Arabidopsis. Plants with decreased methylation displayed a number of phenotypic and developmental abnormalities, including reduced apical dominance, smaller plant size, altered leaf size and shape, decreased fertility, and altered powering time. Floral organs showed homeotic transformations that were associated with ectopic expression of the floral homeotic genes AGAMOUS and APETALA3 in leaf tissue. These observations suggest that DNA methylation plays an important role in regulating many developmental pathways in plants and that the developmental abnormalities seen in the methyltransferase antisense plants may be due to dysregulation of gene expression. Arabidopsis plants transformed with an antisense construct of an Arabidopsis methyltransferase cDNA (METI) have reduced cytosine methylation in CG dinucleotides. Methylation levels in progeny of five independent transformants ranged from 10% to 100% of the wild type. Removal of the antisense construct by segregation in sexual crosses did not fully restore methylation patterns in the progeny, indicating that methylation patterns are subject to meiotic inheritance in Arabidopsis. Plants with decreased methylation displayed a number of phenotypic and developmental abnormalities, including reduced apical dominance, smaller plant size, altered leaf size and shape, decreased fertility, and altered flowering time. Floral organs showed homeotic transformations that were associated with ectopic expression of the floral homeotic genes AGAMOUS and APETALA3 in leaf tissue. These observations suggest that DNA methylation plays an important role in regulating many developmental pathways in plants and that the developmental abnormalities seen in the methyltransferase antisense plants may be due to dysregulation of gene expression.Arabidopsis plants transformed with an antisense construct of an Arabidopsis methyltransferase cDNA (METI) have reduced cytosine methylation in CG dinucleotides. Methylation levels in progeny of five independent transformants ranged from 10% to 100% of the wild type. Removal of the antisense construct by segregation in sexual crosses did not fully restore methylation patterns in the progeny, indicating that methylation patterns are subject to meiotic inheritance in Arabidopsis. Plants with decreased methylation displayed a number of phenotypic and developmental abnormalities, including reduced apical dominance, smaller plant size, altered leaf size and shape, decreased fertility, and altered flowering time. Floral organs showed homeotic transformations that were associated with ectopic expression of the floral homeotic genes AGAMOUS and APETALA3 in leaf tissue. These observations suggest that DNA methylation plays an important role in regulating many developmental pathways in plants and that the developmental abnormalities seen in the methyltransferase antisense plants may be due to dysregulation of gene expression. |
| Author | Finnegan, E.J. (Commonwealth Scientific and Industrial Research Organization, Canberra, ACT, Australia.) Peacock, W.J Dennis, E.S |
| AuthorAffiliation | Commonwealth Scientific and Industrial Research Organization, Division of Plant Industry, Canberra, Australia |
| AuthorAffiliation_xml | – name: Commonwealth Scientific and Industrial Research Organization, Division of Plant Industry, Canberra, Australia |
| Author_xml | – sequence: 1 fullname: Finnegan, E.J. (Commonwealth Scientific and Industrial Research Organization, Canberra, ACT, Australia.) – sequence: 2 fullname: Peacock, W.J – sequence: 3 fullname: Dennis, E.S |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/8710891$$D View this record in MEDLINE/PubMed |
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| Snippet | Arabidopsis plants transformed with an antisense construct of an Arabidopsis methyltransferase cDNA (METI) have reduced cytosine methylation in CG... Results of a study suggest that DNA methylation plays an important role in regulating many developmental pathways in plants, and that the developmental... |
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| SubjectTerms | 5-Methylcytosine abnormal development ADN analogs & derivatives ANATOMIA DE LA PLANTA ANATOMIE VEGETALE antisense DNA apical dominance Arabidopsis Arabidopsis - genetics Arabidopsis - growth & development ARABIDOPSIS THALIANA Botany complementary DNA Cytosine Cytosine - analogs & derivatives Cytosine - metabolism Deoxyribonucleic acid DNA DNA (Cytosine-5-)-Methyltransferases DNA (Cytosine-5-)-Methyltransferases - genetics DNA (Cytosine-5-)-Methyltransferases - metabolism DNA - metabolism DNA methylation DNA, Antisense DNA-binding proteins Enzymes ETAPAS DE DESARROLLO DE LA PLANTA EXPRESION GENICA EXPRESSION DES GENES FERTILIDAD FERTILITE FLORACION FLORAISON Flowers Flowers & plants GENE Gene expression Gene Expression Regulation, Developmental GENES genetics growth & development homeotic genes INHIBICION INHIBITION Leaves metabolism Methylation Mutation Petals Phenotype Phenotypes plant development Plant growth plant morphology Plant reproduction PLANTAS TRANSGENICAS PLANTE TRANSGENIQUE Plants Plants, Genetically Modified PROTEINAS AGLUTINANTES PROTEINE DE LIAISON RAMIFICACION RAMIFICATION RNA, Messenger RNA, Messenger - genetics RNA, Plant RNA, Plant - genetics STADE DE DEVELOPPEMENT VEGETAL structural genes TEMPS TIEMPO transcription factors TRANSFERASAS TRANSFERASE transferases Transgenes transgenic plants TRASTORNOS DEL CRECIMIENTO TROUBLE DE LA CROISSANCE |
| Title | Reduced DNA methylation in Arabidopsis thaliana results in abnormal plant development |
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