Embedding Synthetic Microvascular Networks in Poly(Lactic Acid) Substrates with Rounded Cross-Sections for Cell Culture Applications
Synthetic microvascular networks are essential to enable in vitro studies of cell biology, biophysics, hemodynamics, and drug discovery, as well as in applications involving tissue engineering and artificial vasculature. But current limitations make it challenging to construct networks incorporating...
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| Published in | PloS one Vol. 8; no. 9; p. e73188 |
|---|---|
| Main Authors | , , , , |
| Format | Journal Article |
| Language | English |
| Published |
United States
Public Library of Science
02.09.2013
Public Library of Science (PLoS) |
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| Abstract | Synthetic microvascular networks are essential to enable in vitro studies of cell biology, biophysics, hemodynamics, and drug discovery, as well as in applications involving tissue engineering and artificial vasculature. But current limitations make it challenging to construct networks incorporating a hierarchy of microchannel diameters that possess cell-favored circular cross-sectional topographies. We report a new approach that overcomes these limitations by employing pressure-assisted expansion of biocompatible degradable poly(lactic acid) (PLA) substrates. This single-step process is straightforward and highly controllable, making it possible to simultaneously shape the interior topology of branched 3D and pseudo-3D microchannel networks across wide range of diameters. We further demonstrate in vitro culture of confluent endothelial cell monolayers in microchannel networks treated by this process, suggesting potential as a tool to help generate bio-mimicking vascular-like environments. |
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| AbstractList | Synthetic microvascular networks are essential to enable in vitro studies of cell biology, biophysics, hemodynamics, and drug discovery, as well as in applications involving tissue engineering and artificial vasculature. But current limitations make it challenging to construct networks incorporating a hierarchy of microchannel diameters that possess cell-favored circular cross-sectional topographies. We report a new approach that overcomes these limitations by employing pressure-assisted expansion of biocompatible degradable poly(lactic acid) (PLA) substrates. This single-step process is straightforward and highly controllable, making it possible to simultaneously shape the interior topology of branched 3D and pseudo-3D microchannel networks across wide range of diameters. We further demonstrate in vitro culture of confluent endothelial cell monolayers in microchannel networks treated by this process, suggesting potential as a tool to help generate bio-mimicking vascular-like environments. Synthetic microvascular networks are essential to enable in vitro studies of cell biology, biophysics, hemodynamics, and drug discovery, as well as in applications involving tissue engineering and artificial vasculature. But current limitations make it challenging to construct networks incorporating a hierarchy of microchannel diameters that possess cell-favored circular cross-sectional topographies. We report a new approach that overcomes these limitations by employing pressure-assisted expansion of biocompatible degradable poly(lactic acid) (PLA) substrates. This single-step process is straightforward and highly controllable, making it possible to simultaneously shape the interior topology of branched 3D and pseudo-3D microchannel networks across wide range of diameters. We further demonstrate in vitro culture of confluent endothelial cell monolayers in microchannel networks treated by this process, suggesting potential as a tool to help generate bio-mimicking vascular-like environments. Synthetic microvascular networks are essential to enable in vitro studies of cell biology, biophysics, hemodynamics, and drug discovery, as well as in applications involving tissue engineering and artificial vasculature. But current limitations make it challenging to construct networks incorporating a hierarchy of microchannel diameters that possess cell-favored circular cross-sectional topographies. We report a new approach that overcomes these limitations by employing pressure-assisted expansion of biocompatible degradable poly(lactic acid) (PLA) substrates. This single-step process is straightforward and highly controllable, making it possible to simultaneously shape the interior topology of branched 3D and pseudo-3D microchannel networks across wide range of diameters. We further demonstrate in vitro culture of confluent endothelial cell monolayers in microchannel networks treated by this process, suggesting potential as a tool to help generate bio-mimicking vascular-like environments.Synthetic microvascular networks are essential to enable in vitro studies of cell biology, biophysics, hemodynamics, and drug discovery, as well as in applications involving tissue engineering and artificial vasculature. But current limitations make it challenging to construct networks incorporating a hierarchy of microchannel diameters that possess cell-favored circular cross-sectional topographies. We report a new approach that overcomes these limitations by employing pressure-assisted expansion of biocompatible degradable poly(lactic acid) (PLA) substrates. This single-step process is straightforward and highly controllable, making it possible to simultaneously shape the interior topology of branched 3D and pseudo-3D microchannel networks across wide range of diameters. We further demonstrate in vitro culture of confluent endothelial cell monolayers in microchannel networks treated by this process, suggesting potential as a tool to help generate bio-mimicking vascular-like environments. |
| Audience | Academic |
| Author | Jayaraman, Arul Kim, Jeongyun Huang, Jen-Huang Ding, Yufang Ugaz, Victor M. |
| AuthorAffiliation | Institute for Frontier Medical Sciences, Kyoto University, Japan 2 Department of Biomedical Engineering, Texas A&M University, College Station, Texas, United States of America 1 Artie McFerrin Department of Chemical Engineering, Texas A&M University, College Station, Texas, United States of America |
| AuthorAffiliation_xml | – name: 1 Artie McFerrin Department of Chemical Engineering, Texas A&M University, College Station, Texas, United States of America – name: 2 Department of Biomedical Engineering, Texas A&M University, College Station, Texas, United States of America – name: Institute for Frontier Medical Sciences, Kyoto University, Japan |
| Author_xml | – sequence: 1 givenname: Jen-Huang surname: Huang fullname: Huang, Jen-Huang – sequence: 2 givenname: Jeongyun surname: Kim fullname: Kim, Jeongyun – sequence: 3 givenname: Yufang surname: Ding fullname: Ding, Yufang – sequence: 4 givenname: Arul surname: Jayaraman fullname: Jayaraman, Arul – sequence: 5 givenname: Victor M. surname: Ugaz fullname: Ugaz, Victor M. |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/24023829$$D View this record in MEDLINE/PubMed |
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| ContentType | Journal Article |
| Copyright | COPYRIGHT 2013 Public Library of Science 2013 Huang et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. 2013 Huang et al 2013 Huang et al |
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| Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23 Competing Interests: The authors have declared that no competing interests exist. Conceived and designed the experiments: J-HH JK AJ VMU. Performed the experiments: J-HH JK YD. Analyzed the data: J-HH JK YD. Wrote the paper: J-HH AJ VMU. |
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| SubjectTerms | Acids Animals Aorta - cytology Biocompatibility Biocompatible Materials - metabolism Biomedical engineering Biomimetics - methods Biophysics Blood Vessels - cytology Cattle Cell culture Cell Culture Techniques - methods Chemical engineering Cross-sections Embedding Endothelial cells Endothelial Cells - cytology Endothelial Cells - metabolism Hemodynamics Lactic acid Lactic Acid - metabolism Lasers Methods Microtechnology - methods Microvasculature Mimicry Networks Physiological aspects Polyesters Polylactic acid Polymers - metabolism Substrates Technology application Time Factors Tissue engineering Topology |
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| Title | Embedding Synthetic Microvascular Networks in Poly(Lactic Acid) Substrates with Rounded Cross-Sections for Cell Culture Applications |
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