Distinct temporal roles for the promyelocytic leukaemia (PML) protein in the sequential regulation of intracellular host immunity to HSV-1 infection

• Published in: PLoS pathogens Vol. 14; no. 1; p. e1006769
• Main Authors: Alandijany, Thamir, Roberts, Ashley P E, Conn, Kristen L, Loney, Colin, McFarlane, Steven, Orr, Anne, Boutell, Chris
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 01.01.2018; Public Library of Science (PLoS)
• Subjects: Analysis; Biology and Life Sciences; Cell Line; Cell Line, Transformed; Cells, Cultured; Chemical synthesis; Click Chemistry; Cytokines; Cytomegalovirus; Deoxyribonucleic acid; DNA; Gene Deletion; Gene expression; Gene Expression Regulation, Viral - drug effects; Genetic aspects; Genomes; Herpes simplex; Herpes Simplex - drug therapy; Herpes Simplex - metabolism; Herpes Simplex - pathology; Herpes Simplex - virology; Herpes viruses; Herpesvirus 1, Human - growth & development; Herpesvirus 1, Human - physiology; Herpesvirus infections; Host-Pathogen Interactions - drug effects; Host-virus relationships; Humans; Immune response; Immune system; Immunity; Immunity, Innate - drug effects; Inclusion Bodies, Viral - drug effects; Inclusion Bodies, Viral - metabolism; Inclusion Bodies, Viral - pathology; Inclusion Bodies, Viral - virology; Infections; Innate immunity; Interferon; Intracellular; Kinases; Kinetics; Labeling; Labelling; Leukemia; Lysogeny - drug effects; Medicine and Health Sciences; Multiplicity of infection; Mutation; Nuclear Proteins - antagonists & inhibitors; Nuclear Proteins - genetics; Nuclear Proteins - metabolism; Nuclei; Nucleic acids; Phosphoproteins - antagonists & inhibitors; Phosphoproteins - genetics; Phosphoproteins - metabolism; Promyelocytic Leukemia Protein - antagonists & inhibitors; Promyelocytic Leukemia Protein - genetics; Promyelocytic Leukemia Protein - metabolism; Proteins; Recruitment; Regulators; Replication; Reverse Transcriptase Inhibitors - pharmacology; RNA Interference; Sensors; Supervision; Tumor suppressor genes; Ubiquitin-Protein Ligases - genetics; Ubiquitin-Protein Ligases - metabolism; Viral Proteins - genetics; Viral Proteins - metabolism; Virus Internalization - drug effects; Virus replication; Virus Replication - drug effects; Viruses; Glasgow Scotland; United Kingdom > UK; Scotland

Detection of viral nucleic acids plays a critical role in the induction of intracellular host immune defences. However, the temporal recruitment of immune regulators to infecting viral genomes remains poorly defined due to the technical difficulties associated with low genome copy-number detection....