The Atlantic Bonito (Sarda sarda, Bloch 1793) transcriptome and detection of differential expression during larvae development
The Atlantic bonito (Sarda sarda, Bloch 1793) belongs to the important marine fish species with a wide geographical distribution covering the Atlantic Ocean, the Mediterranean and its bordering seas. Aquaculture practices for this species are still in their infancies and scientific studies are seldo...
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Published in | PloS one Vol. 9; no. 2; p. e87744 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | English |
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04.02.2014
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Abstract | The Atlantic bonito (Sarda sarda, Bloch 1793) belongs to the important marine fish species with a wide geographical distribution covering the Atlantic Ocean, the Mediterranean and its bordering seas. Aquaculture practices for this species are still in their infancies and scientific studies are seldom undertaken, mainly because of difficulties in sampling. Thus for small tuna species like the Atlantic bonito only little is known about its biology and regarding the molecular background even less information is available. In the production of marine fish it is known that the most critical period is the larval stages, as high growth rates as well as significant developmental changes take place. In this study we have investigated the transcriptome of the Atlantic bonito of five larvae stages applying Illumina sequencing technology. For non-model species like aquaculture species, transcriptome analysis of RNA samples from individuals using Illumina sequencing technology is technically efficient and cost effective. In the present study a total number of 169,326,711 paired-end reads with a read length of 100 base pairs were generated resulting in a reference transcriptome of 68,220 contigs with an average length of 2054 base pairs. For differential expression analyses single end reads were obtained from different developmental stages and mapped to the constructed reference transcriptome. Differential expression analyses revealed in total 18,657 differentially expressed transcripts and were assigned to five distinguished groups. Each of the five clusters shows stage specific gene expression. We present for the first time in the Atlantic bonito an extensive RNA-Seq based characterization of its transcriptome as well as significant information on differential expression among five developmental larvae stages. The generated transcripts, including SNP and microsatellite information for candidate molecular markers and gene expression information will be a valuable resource for future genetic and molecular studies. |
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AbstractList | The Atlantic bonito (Sarda sarda, Bloch 1793) belongs to the important marine fish species with a wide geographical distribution covering the Atlantic Ocean, the Mediterranean and its bordering seas. Aquaculture practices for this species are still in their infancies and scientific studies are seldom undertaken, mainly because of difficulties in sampling. Thus for small tuna species like the Atlantic bonito only little is known about its biology and regarding the molecular background even less information is available. In the production of marine fish it is known that the most critical period is the larval stages, as high growth rates as well as significant developmental changes take place. In this study we have investigated the transcriptome of the Atlantic bonito of five larvae stages applying Illumina sequencing technology. For non-model species like aquaculture species, transcriptome analysis of RNA samples from individuals using Illumina sequencing technology is technically efficient and cost effective. In the present study a total number of 169,326,711 paired-end reads with a read length of 100 base pairs were generated resulting in a reference transcriptome of 68,220 contigs with an average length of 2054 base pairs. For differential expression analyses single end reads were obtained from different developmental stages and mapped to the constructed reference transcriptome. Differential expression analyses revealed in total 18,657 differentially expressed transcripts and were assigned to five distinguished groups. Each of the five clusters shows stage specific gene expression. We present for the first time in the Atlantic bonito an extensive RNA-Seq based characterization of its transcriptome as well as significant information on differential expression among five developmental larvae stages. The generated transcripts, including SNP and microsatellite information for candidate molecular markers and gene expression information will be a valuable resource for future genetic and molecular studies. The Atlantic bonito ( Sarda sarda , Bloch 1793) belongs to the important marine fish species with a wide geographical distribution covering the Atlantic Ocean, the Mediterranean and its bordering seas. Aquaculture practices for this species are still in their infancies and scientific studies are seldom undertaken, mainly because of difficulties in sampling. Thus for small tuna species like the Atlantic bonito only little is known about its biology and regarding the molecular background even less information is available. In the production of marine fish it is known that the most critical period is the larval stages, as high growth rates as well as significant developmental changes take place. In this study we have investigated the transcriptome of the Atlantic bonito of five larvae stages applying Illumina sequencing technology. For non-model species like aquaculture species, transcriptome analysis of RNA samples from individuals using Illumina sequencing technology is technically efficient and cost effective. In the present study a total number of 169,326,711 paired-end reads with a read length of 100 base pairs were generated resulting in a reference transcriptome of 68,220 contigs with an average length of 2054 base pairs. For differential expression analyses single end reads were obtained from different developmental stages and mapped to the constructed reference transcriptome. Differential expression analyses revealed in total 18,657 differentially expressed transcripts and were assigned to five distinguished groups. Each of the five clusters shows stage specific gene expression. We present for the first time in the Atlantic bonito an extensive RNA-Seq based characterization of its transcriptome as well as significant information on differential expression among five developmental larvae stages. The generated transcripts, including SNP and microsatellite information for candidate molecular markers and gene expression information will be a valuable resource for future genetic and molecular studies. |
Audience | Academic |
Author | Papandroulakis, Nikos Sarropoulou, Elena Moghadam, Hooman K Makridis, Pavlos Ortega Garcia, Aurelio De la Gándara, Fernando |
AuthorAffiliation | 1 Institute of Marine Biology, Biotechnology and Aquaculture, Hellenic Centre for Marine Research, Heraklion, Greece National University of Singapore, Singapore 2 Instituto Español de Oceanografia (IEO), Centro Oceanografico de Murcia, Carretera de La Azohia, Puerto de Mazarron, Spain |
AuthorAffiliation_xml | – name: 1 Institute of Marine Biology, Biotechnology and Aquaculture, Hellenic Centre for Marine Research, Heraklion, Greece – name: National University of Singapore, Singapore – name: 2 Instituto Español de Oceanografia (IEO), Centro Oceanografico de Murcia, Carretera de La Azohia, Puerto de Mazarron, Spain |
Author_xml | – sequence: 1 givenname: Elena surname: Sarropoulou fullname: Sarropoulou, Elena organization: Institute of Marine Biology, Biotechnology and Aquaculture, Hellenic Centre for Marine Research, Heraklion, Greece – sequence: 2 givenname: Hooman K surname: Moghadam fullname: Moghadam, Hooman K organization: Institute of Marine Biology, Biotechnology and Aquaculture, Hellenic Centre for Marine Research, Heraklion, Greece – sequence: 3 givenname: Nikos surname: Papandroulakis fullname: Papandroulakis, Nikos organization: Institute of Marine Biology, Biotechnology and Aquaculture, Hellenic Centre for Marine Research, Heraklion, Greece – sequence: 4 givenname: Fernando surname: De la Gándara fullname: De la Gándara, Fernando organization: Instituto Español de Oceanografia (IEO), Centro Oceanografico de Murcia, Carretera de La Azohia, Puerto de Mazarron, Spain – sequence: 5 givenname: Aurelio surname: Ortega Garcia fullname: Ortega Garcia, Aurelio organization: Instituto Español de Oceanografia (IEO), Centro Oceanografico de Murcia, Carretera de La Azohia, Puerto de Mazarron, Spain – sequence: 6 givenname: Pavlos surname: Makridis fullname: Makridis, Pavlos organization: Institute of Marine Biology, Biotechnology and Aquaculture, Hellenic Centre for Marine Research, Heraklion, Greece |
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CitedBy_id | crossref_primary_10_1152_physiolgenomics_00001_2015 crossref_primary_10_1534_g3_116_036699 crossref_primary_10_1016_j_aquaculture_2020_735654 crossref_primary_10_1016_j_margen_2015_03_002 crossref_primary_10_1093_gbe_evw211 crossref_primary_10_1016_j_margen_2020_100834 crossref_primary_10_1016_j_aquaculture_2022_738964 crossref_primary_10_1111_1755_0998_12451 crossref_primary_10_1016_j_aquaculture_2024_740979 crossref_primary_10_1016_j_margen_2014_06_009 crossref_primary_10_1016_j_fsi_2014_11_018 crossref_primary_10_1016_j_gene_2018_04_054 |
Cites_doi | 10.1186/1471-2164-10-157 10.1111/j.1745-4514.2009.00247.x 10.1093/nar/gkq817 10.1186/gb-2010-11-10-r106 10.1371/journal.pone.0049357 10.1111/j.1439-0426.2008.01102.x 10.1111/j.1095-8649.2006.01090.x 10.1007/s10126-011-9375-6 10.1007/s10126-011-9422-3 10.1186/gb-2009-10-3-r25 10.1186/1471-2164-12-53 10.1186/1471-2164-13-181 10.1101/gr.885403 10.1152/physiolgenomics.00139.2005 10.1002/dvdy.20210 10.1038/nbt.1883 10.1186/1471-2105-12-323 10.1093/bioinformatics/btl158 10.1186/1471-2164-10-502 10.1007/s10126-012-9466-z 10.1155/2008/619832 10.1186/gb-2010-11-12-220 10.1038/sj.emboj.7601821 10.1101/gr.124321.111 10.1371/journal.pgen.0010029 10.1093/nar/gks540 10.1111/j.1095-8649.2000.tb00781.x 10.1016/j.ympev.2009.10.001 10.1002/aja.1002030302 10.1016/S1546-5098(08)60214-9 10.1016/j.gene.2006.09.012 10.1186/1471-2164-13-595 10.1016/S0022-2836(05)80360-2 |
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Copyright | COPYRIGHT 2014 Public Library of Science 2014 Sarropoulou et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. 2014 Sarropoulou et al 2014 Sarropoulou et al |
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Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Conceived and designed the experiments: ES NP PM. Performed the experiments: ES NP. Analyzed the data: ES HKM. Contributed reagents/materials/analysis tools: FDG AOG. Wrote the paper: ES NP PM. Competing Interests: The authors have declared that no competing interests exist. Current address: Nofima, Osloveien1, AS, Norway. |
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References | S Mathavan (ref9) 2005; 1 M Ferg (ref15) 2007; 26 C Wen (ref16) 2005; 232 S Liu (ref38) 2010; 39 ref34 B Langmead (ref29) 2009; 10 K Tamura (ref36) 1993; 10 DJ McFarlane (ref5) 2000; 57 B Li (ref30) 2011; 12 (ref35) 2011; 1 SF Altschul (ref32) 1990; 215 M Lohse (ref25) 2012; 40 K Tamura (ref37) 2011; 28 J Lo (ref8) 2003; 13 S Liu (ref17) 2012; 12 E Sarropoulou (ref11) 2011; 13 S Anders (ref31) 2010; 11 ref24 ref23 R Andreassen (ref42) 2009; 10 C Ateş (ref1) 2008; 24 N Zaboukas (ref2) 2006; 69 M Yufera (ref13) 2011; 14 J Bai (ref14) 2007; 386 ref28 BM Lane (ref41) 2012; 7 MG Grabherr (ref26) 2011; 29 E Sarropoulou (ref20) 2009; 10 W Li (ref27) 2006; 22 A Oshlack (ref39) 2010; 11 D Garcia de la Serrana (ref19) 2012; 13 S Tarazona (ref40) 2011; 21 ref4 E Sarropoulou (ref10) 2005; 23 ref3 ref6 E Sarropoulou (ref12) 2010; 55 S Liu (ref18) 2012; 13 E Sarropoulou (ref21) 2012; 14 SY Erkan Tosun (ref22) 2009; 33 A Conesa (ref33) 2008; 2008 CB Kimmel (ref7) 1995; 203 |
References_xml | – volume: 10 start-page: 157 year: 2009 ident: ref20 article-title: Profiling of infection specific mRNA transcripts of the European seabass Dicentrarchus labrax. publication-title: BMC Genomics doi: 10.1186/1471-2164-10-157 contributor: fullname: E Sarropoulou – volume: 33 start-page: 728 year: 2009 ident: ref22 article-title: Keeping quality of different packaged salted atlantic bonito “lakerda.” publication-title: Journal of Food Biochemistry doi: 10.1111/j.1745-4514.2009.00247.x contributor: fullname: SY Erkan Tosun – volume: 39 start-page: 578 year: 2010 ident: ref38 article-title: A comparison of RNA-Seq and high-density exon array for detecting differential gene expression between closely related species publication-title: Nucleic Acids Res doi: 10.1093/nar/gkq817 contributor: fullname: S Liu – ident: ref3 – volume: 11 start-page: R106 year: 2010 ident: ref31 article-title: Differential expression analysis for sequence count data publication-title: Genome Biology doi: 10.1186/gb-2010-11-10-r106 contributor: fullname: S Anders – volume: 1 start-page: 409 year: 2011 ident: ref35 article-title: R: A Language and Environment for Statistical Computing publication-title: R Foundation for Statistical Computing – volume: 7 start-page: e49357 year: 2012 ident: ref41 article-title: Otx but not Mitf transcription factors are required for zebrafish retinal pigment epithelium development publication-title: PLoS ONE doi: 10.1371/journal.pone.0049357 contributor: fullname: BM Lane – volume: 24 start-page: 546 year: 2008 ident: ref1 article-title: Age and growth of Atlantic bonito (Sarda sarda Bloch, 1793) in the Sea of Marmara and Black Sea, Turkey publication-title: Journal of Applied Ichthyology doi: 10.1111/j.1439-0426.2008.01102.x contributor: fullname: C Ateş – volume: 69 start-page: 347 year: 2006 ident: ref2 article-title: Biochemical composition of the Atlantic bonito Sarda sarda from the Aegean Sea (eastern Mediterranean Sea) in different stages of sexual maturity publication-title: Jounral of Fish Biology doi: 10.1111/j.1095-8649.2006.01090.x contributor: fullname: N Zaboukas – volume: 10 start-page: 512 year: 1993 ident: ref36 article-title: Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees publication-title: Molecular Biology and Evolution contributor: fullname: K Tamura – volume: 13 start-page: 1115 year: 2011 ident: ref11 article-title: Functional divergences of GAPDH isoforms during early development in two perciform fish species publication-title: Marine biotechnology New York NY doi: 10.1007/s10126-011-9375-6 contributor: fullname: E Sarropoulou – volume: 14 start-page: 423 year: 2011 ident: ref13 article-title: Transcriptomic characterization of the larval stage in gilthead seabream (Sparus aurata) by 454 pyrosequencing publication-title: Mar Biotechnol (NY) doi: 10.1007/s10126-011-9422-3 contributor: fullname: M Yufera – ident: ref24 – volume: 10 start-page: R25 year: 2009 ident: ref29 article-title: Ultrafast and memory-efficient alignment of short DNA sequences to the human genome publication-title: Genome Biology doi: 10.1186/gb-2009-10-3-r25 contributor: fullname: B Langmead – volume: 12 start-page: 53 year: 2012 ident: ref17 article-title: Generation of genome-scale gene-associated SNPs in catfish for the construction of a high-density SNP array publication-title: BMC Genomics doi: 10.1186/1471-2164-12-53 contributor: fullname: S Liu – volume: 13 start-page: 181 year: 2012 ident: ref19 article-title: Fast skeletal muscle transcriptome of the gilthead sea bream (Sparus aurata) determined by next generation sequencing publication-title: BMC Genomics doi: 10.1186/1471-2164-13-181 contributor: fullname: D Garcia de la Serrana – volume: 13 start-page: 455 year: 2003 ident: ref8 article-title: 15000 unique zebrafish EST clusters and their future use in microarray for profiling gene expression patterns during embryogenesis publication-title: Genome Res doi: 10.1101/gr.885403 contributor: fullname: J Lo – volume: 23 start-page: 182 year: 2005 ident: ref10 article-title: Gene expression profiling of gilthead sea bream during early development and detection of stress-related genes by the application of cDNA microarray technology publication-title: Physiol Genomics doi: 10.1152/physiolgenomics.00139.2005 contributor: fullname: E Sarropoulou – volume: 232 start-page: 171 year: 2005 ident: ref16 article-title: Genome-wide identification of female-enriched genes in zebrafish publication-title: Dev Dyn doi: 10.1002/dvdy.20210 contributor: fullname: C Wen – ident: ref34 – volume: 29 start-page: 644 year: 2011 ident: ref26 article-title: Full-length transcriptome assembly from RNA-Seq data without a reference genome publication-title: Nat Biotechnol doi: 10.1038/nbt.1883 contributor: fullname: MG Grabherr – volume: 12 start-page: 323 year: 2011 ident: ref30 article-title: RSEM: accurate transcript quantification from RNA-Seq data with or without a reference genome publication-title: BMC Bioinformatics doi: 10.1186/1471-2105-12-323 contributor: fullname: B Li – volume: 22 start-page: 1658 year: 2006 ident: ref27 article-title: Cd-hit: a fast program for clustering and comparing large sets of protein or nucleotide sequences publication-title: Bioinformatics doi: 10.1093/bioinformatics/btl158 contributor: fullname: W Li – ident: ref4 – volume: 10 start-page: 502 year: 2009 ident: ref42 article-title: Characterization of full-length sequenced cDNA inserts (FLIcs) from Atlantic salmon (Salmo salar) publication-title: BMC Genomics doi: 10.1186/1471-2164-10-502 contributor: fullname: R Andreassen – volume: 14 start-page: 634 year: 2012 ident: ref21 article-title: Characterization of European sea bass transcripts by RNA SEQ after oral vaccine against V. anguillarum publication-title: Mar Biotechnol (NY) doi: 10.1007/s10126-012-9466-z contributor: fullname: E Sarropoulou – volume: 2008 start-page: 619832 year: 2008 ident: ref33 article-title: Blast2GO: A Comprehensive Suite for Functional Analysis in Plant Genomics publication-title: International journal of plant genomics doi: 10.1155/2008/619832 contributor: fullname: A Conesa – ident: ref28 – volume: 11 start-page: 220 year: 2010 ident: ref39 article-title: From RNA-seq reads to differential expression results publication-title: Genome Biol doi: 10.1186/gb-2010-11-12-220 contributor: fullname: A Oshlack – volume: 26 start-page: 3945 year: 2007 ident: ref15 article-title: The TATA-binding protein regulates maternal mRNA degradation and differential zygotic transcription in zebrafish publication-title: EMBO J doi: 10.1038/sj.emboj.7601821 contributor: fullname: M Ferg – ident: ref23 – volume: 21 start-page: 2213 year: 2011 ident: ref40 article-title: Differential expression in RNA-seq: A matter of depth publication-title: Genome Research doi: 10.1101/gr.124321.111 contributor: fullname: S Tarazona – volume: 28 start-page: 1530 year: 2011 ident: ref37 article-title: MEGA5: Molecular Evolutionary Genetics Analysis using Maximum Likelihood, Evolutionary Distance, and Maximum Parsimony Methods publication-title: Molecular Biology contributor: fullname: K Tamura – volume: 1 start-page: 260 year: 2005 ident: ref9 article-title: Transcriptome analysis of zebrafish embryogenesis using microarrays publication-title: PLoS Genet doi: 10.1371/journal.pgen.0010029 contributor: fullname: S Mathavan – volume: 40 start-page: 1 year: 2012 ident: ref25 article-title: RobiNA: a user-friendly, integrated software solution for RNA-Seq-based transcriptomics publication-title: Nucleic Acids Research doi: 10.1093/nar/gks540 contributor: fullname: M Lohse – volume: 57 start-page: 134 year: 2000 ident: ref5 article-title: Larval growth and development of cultured Pacific bonito publication-title: Journal of fish Biology doi: 10.1111/j.1095-8649.2000.tb00781.x contributor: fullname: DJ McFarlane – volume: 55 start-page: 640 year: 2010 ident: ref12 article-title: Evolution of a multifunctional gene: The warm temperature acclimation protein Wap65 in the European seabass Dicentrarchus labrax publication-title: Molecular Phylogenetics and Evolution doi: 10.1016/j.ympev.2009.10.001 contributor: fullname: E Sarropoulou – volume: 203 start-page: 253 year: 1995 ident: ref7 article-title: Stages of embryonic development of the zebrafish publication-title: Dev Dyn doi: 10.1002/aja.1002030302 contributor: fullname: CB Kimmel – ident: ref6 doi: 10.1016/S1546-5098(08)60214-9 – volume: 386 start-page: 202 year: 2007 ident: ref14 article-title: Profiling of maternal and developmental-stage specific mRNA transcripts in Atlantic halibut Hippoglossus hippoglossus publication-title: Gene doi: 10.1016/j.gene.2006.09.012 contributor: fullname: J Bai – volume: 13 start-page: 595 year: 2012 ident: ref18 article-title: Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote publication-title: BMC Genomics doi: 10.1186/1471-2164-13-595 contributor: fullname: S Liu – volume: 215 start-page: 403 year: 1990 ident: ref32 article-title: Basic local alignment search tool publication-title: Journal of Molecular Biology doi: 10.1016/S0022-2836(05)80360-2 contributor: fullname: SF Altschul |
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Snippet | The Atlantic bonito (Sarda sarda, Bloch 1793) belongs to the important marine fish species with a wide geographical distribution covering the Atlantic Ocean,... The Atlantic bonito ( Sarda sarda , Bloch 1793) belongs to the important marine fish species with a wide geographical distribution covering the Atlantic Ocean,... |
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SubjectTerms | 3' Untranslated Regions Animals Aquaculture Aquaculture practices Base pairs Base Sequence Bioinformatics Biology Biotechnology Cluster Analysis Critical period Developmental stages DNA sequencing Fish Fisheries Fishes Fishes - classification Fishes - genetics Gene expression Gene Expression Profiling Gene Expression Regulation, Developmental Gene Regulatory Networks Gene sequencing Genes Genomes Genomics Geographical distribution High-Throughput Nucleotide Sequencing Integrated software Larva - genetics Larvae Marine biology Marine fish Microsatellite Repeats Mitochondrial DNA Molecular biology Molecular Sequence Annotation Molecular Sequence Data Phylogeny Polymorphism, Single Nucleotide Ribonucleic acid RNA RNA sequencing Salinity Sarda chiliensis Sarda sarda Sequence Alignment Single-nucleotide polymorphism Species Technology Thunnus thynnus Transcriptome Tuna Zebrafish |
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Title | The Atlantic Bonito (Sarda sarda, Bloch 1793) transcriptome and detection of differential expression during larvae development |
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