Detection of Human Parechoviruses from Clinical Stool Samples in Aichi, Japan

• Published in: Journal of Clinical Microbiology Vol. 48; no. 8; pp. 2683 - 2688
• Main Authors: Ito, Miyabi, Yamashita, Teruo, Tsuzuki, Hideaki, Kabashima, Yuka, Hasegawa, Akiko, Nagaya, Satoko, Kawaguchi, Mariko, Kobayashi, Shinichi, Fujiura, Akira, Sakae, Kenji, Minagawa, Hiroko
• Format: Journal Article
• Language: English
• Published: Washington, DC American Society for Microbiology 01.08.2010; American Society for Microbiology (ASM)
• Subjects: 5' Untranslated Regions; Age Factors; Biological and medical sciences; Capsid Proteins - genetics; Cell Culture Techniques; Child, Preschool; Cluster Analysis; Feces - virology; Female; Fundamental and applied biological sciences. Psychology; Gastroenteritis - virology; Genotype; Humans; Infant; Infant, Newborn; Japan - epidemiology; Male; Microbiology; Miscellaneous; Molecular Sequence Data; Neutralization Tests; Parechovirus - growth & development; Parechovirus - isolation & purification; Picornaviridae Infections - diagnosis; Picornaviridae Infections - epidemiology; Picornaviridae Infections - pathology; Picornaviridae Infections - virology; Polymorphism, Genetic; Respiratory Tract Infections - virology; Reverse Transcriptase Polymerase Chain Reaction; RNA, Viral - genetics; Sequence Analysis, DNA; Sequence Homology; Serotyping; Virology; Virology - methods; Asia; Japan; Far east; Virus; Human; Parechovirus; Feces; Detection; Picornaviridae

Between April 1999 and March 2008, a total of 4,976 stool specimens collected from patients with suspected viral infection through infectious agent surveillance in Aichi, Japan, were tested for the presence of human parechoviruses (HPeVs). We detected HPeVs in 110 samples by either cell culture, rev...