Disruption of HPV16-E7 by CRISPR/Cas System Induces Apoptosis and Growth Inhibition in HPV16 Positive Human Cervical Cancer Cells

• Published in: BioMed research international Vol. 2014; no. 2014; pp. 1 - 9
• Main Authors: Zhu, Da, Yu, Lan, Hu, Zheng, Ding, Wencheng, Wang, Xiaoli, Zhang, Changlin, Wang, Liming, Jiang, Xiaohui, Shen, Hui, He, Dan, Li, Kezhen, Xi, Ling, Ma, Ding, Wang, Hui
• Format: Journal Article
• Language: English
• Published: Cairo, Egypt Hindawi Puplishing Corporation 01.01.2014; Hindawi Publishing Corporation; John Wiley & Sons, Inc
• Subjects: Apoptosis; Apoptosis - genetics; Biomedical research; Cancer cells; Cell Proliferation - genetics; Cervical cancer; Colleges & universities; CRISPR-Cas Systems - genetics; Female; Genes; HEK293 Cells; Human papillomavirus; Human papillomavirus 16; Human papillomavirus 16 - genetics; Human papillomavirus 16 - pathogenicity; Humans; Mutation; Papillomavirus E7 Proteins - antagonists & inhibitors; Papillomavirus E7 Proteins - genetics; Papillomaviruses; Plasmids; Uterine Cervical Neoplasms - genetics; Uterine Cervical Neoplasms - pathology; Uterine Cervical Neoplasms - virology; China
• ISSN: 2314-6133; 2314-6141; 2314-6141
• DOI: 10.1155/2014/612823

High-risk human papillomavirus (HR-HPV) has been recognized as a major causative agent for cervical cancer. Upon HPV infection, early genes E6 and E7 play important roles in maintaining malignant phenotype of cervical cancer cells. By using clustered regularly interspaced short palindromic repeats-...