Osmoregulation in Saccharomyces cerevisiae Studies on the osmotic induction of glycerol production and glycerol 3-phosphate dehydrogenase (NAD+)

• Published in: FEBS letters Vol. 286; no. 1-2; pp. 13 - 17
• Main Authors: André, Lars, Hemming, Anna, Adler, Lennart
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 29.07.1991; Elsevier
• Subjects: Biological and medical sciences; Cell physiology; Ethanol - metabolism; Fundamental and applied biological sciences. Psychology; Glycerol; Glycerol - metabolism; Glycerol 3-phosphate dehydrogenase; Glycerol-3-Phosphate Dehydrogenase (NAD+); Glycerolphosphate Dehydrogenase - metabolism; Immunoblotting; Miscellaneous; Molecular and cellular biology; Osmoregulation; Osmotic Pressure; Raffinose - metabolism; Saccharomyces cerevisiae; Saccharomyces cerevisiae - enzymology; Saccharomyces cerevisiae - growth & development; Saccharomyces cerevisiae - metabolism; Trehalose; Trehalose - metabolism; Water-Electrolyte Balance; Glycerol; Osmoregulation; Trehalose; Saccharomyces cerevisiae; Glycerol 3-phosphate dehydrogenase; Fungi; Yeast; Enzymatic activity; Glycerol-3-phosphate dehydrogenase (NAD+); Microorganism culture; Immunoblotting assay; Ascomycetes; Thallophyta

Production of glycerol and a key enzyme in glycerol production, glycerol 3-phosphate dehydrogenase (NAD+) (GPD), was studied in Saccharomyces cerevisiae cultured in basal media or media of high salinity, with glucose, raffinose or ethanol as the sole carbon source. At high salinity, glycerol product...