Heat-shock puff 93 D from Drosophila melanogaster: accumulation of a RNP-specific antigen associated with giant particles of possible storage function

• Published in: The EMBO journal Vol. 2; no. 10; pp. 1747 - 1751
• Main Authors: Dangli, A, Grond, C, Kloetzel, P, Bautz, E.K.F
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group 01.01.1983
• Subjects: Analytical, structural and metabolic biochemistry; animal breeding; animal genetics; Animals; Antibodies, Monoclonal; arthropods; Biological and medical sciences; Chromosomes - ultrastructure; Drosophila melanogaster; Drosophila melanogaster - genetics; Drosophilidae; entomology; Enzyme-Linked Immunosorbent Assay; Fundamental and applied biological sciences. Psychology; Heat-Shock Proteins - genetics; Hot Temperature; Larva - analysis; Microscopy, Electron; Nucleic acids; Ribonucleoproteins - analysis; Ribonucleoproteins - genetics; Rna, ribonucleoproteins; Ribonucleoprotein; Puff; Transcription; Polytene chromosome; Insecta; Giant particle; Monoclonal antibody; Indirect immunofluorescence; Antigen; Arthropoda; Thermal shock; RNA polymerase 2; Invertebrata; Localization; Drosophila melanogaster; ELISA assay; Diptera; Nuclear protein

The monoclonal antibody P11 is directed against a 38 000 dalton protein of Drosophila melanogaster. On polytene chromosomes this protein is present in a subset of the RNA polymerase II‐containing loci. Here we show by density centrifugation and enzyme‐linked immunosorbent assay tests that the P11 an...