Archaeal Signal Transduction: Impact of Protein Phosphatase Deletions on Cell Size, Motility, and Energy Metabolism in Sulfolobus acidocaldarius

• Published in: Molecular & cellular proteomics Vol. 12; no. 12; pp. 3908 - 3923
• Main Authors: Reimann, Julia, Esser, Dominik, Orell, Alvaro, Amman, Fabian, Pham, Trong Khoa, Noirel, Josselin, Lindås, Ann-Christin, Bernander, Rolf, Wright, Phillip C., Siebers, Bettina, Albers, Sonja-Verena
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.12.2013; The American Society for Biochemistry and Molecular Biology
• Subjects: Archaeal Proteins - genetics; Archaeal Proteins - metabolism; Electron Transport - genetics; Energy Metabolism - genetics; Gene Deletion; Gene Expression Profiling; Gene Expression Regulation, Archaeal; Isoenzymes - genetics; Isoenzymes - metabolism; Kinetics; Molecular Sequence Annotation; Movement; Phosphoproteins - genetics; Phosphoproteins - metabolism; Phosphorylation; Protein Phosphatase 2 - genetics; Protein Phosphatase 2 - metabolism; Signal Transduction - genetics; Sulfolobus acidocaldarius - enzymology; Sulfolobus acidocaldarius - genetics; Sulfolobus acidocaldarius - ultrastructure; Transcriptome

In this study, the in vitro and in vivo functions of the only two identified protein phosphatases, Saci-PTP and Saci-PP2A, in the crenarchaeal model organism Sulfolobus acidocaldarius were investigated. Biochemical characterization revealed that Saci-PTP is a dual-specific phosphatase (against pSer/...