Morphological and Behavioral Impact of AAV2/5-Mediated Overexpression of Human Wildtype Alpha-Synuclein in the Rat Nigrostriatal System

• Published in: PloS one Vol. 8; no. 11; p. e81426
• Main Authors: Gombash, Sara E., Manfredsson, Fredric P., Kemp, Christopher J., Kuhn, Nathan C., Fleming, Sheila M., Egan, Ann E., Grant, Laura M., Ciucci, Michelle R., MacKeigan, Jeffrey P., Sortwell, Caryl E.
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 27.11.2013; Public Library of Science (PLoS)
• Subjects: alpha-Synuclein - genetics; Animal models; Animals; Behavior, Animal; Cell Death; Cylinders; Cytomegalovirus; Degeneration; Denervation; Departments; Dependovirus - genetics; Dopamine; Fluorescence; Forelimb - physiology; Gene Expression; Genetic Vectors - genetics; Green fluorescent protein; Human behavior; Humans; Hydroxylase; Impairment; Injection; Laboratories; Male; Medicine; Movement disorders; Neostriatum; Neurodegeneration; Neurodegenerative diseases; Neurons; Neurons - cytology; Neurosciences; Parkinson's disease; Pathogenesis; Pathology; Proteins; Rats; Rats, Sprague-Dawley; Rodents; Science; Social research; Studies; Substantia nigra; Substantia Nigra - cytology; Substantia Nigra - metabolism; Substantia Nigra - physiology; Synuclein; Time Factors; Tyrosine; Tyrosine 3-monooxygenase; Tyrosine 3-Monooxygenase - immunology; Viruses; Vocalization, Animal; Ohio; United States > US; Wisconsin; Michigan

The discovery of the involvement of alpha-synuclein (α-syn) in Parkinson's disease (PD) pathogenesis has resulted in the development and use of viral vector-mediated α-syn overexpression rodent models. The goal of these series of experiments was to characterize the neurodegeneration and functio...