Overproduction of Heterologous Mannitol 1-Phosphatase: a Key Factor for Engineering Mannitol Production by Lactococcus lactis

• Published in: Applied and Environmental Microbiology Vol. 71; no. 3; pp. 1507 - 1514
• Main Authors: Wisselink, H. Wouter, Moers, Antoine P. H. A, Mars, Astrid E, Hoefnagel, Marcel H. N, de Vos, Willem M, Hugenholtz, Jeroen
• Format: Journal Article
• Language: English
• Published: Washington, DC American Society for Microbiology 01.03.2005
• Subjects: acid bacteria; alcohol oxidoreductases; Animals; Bacteria; Base Sequence; Biological and medical sciences; biosynthesis; Biotechnology; Cloning; complete genome sequence; controlled gene-expression; deficient; Eimeria tenella; Eimeria tenella - enzymology; Eimeria tenella - genetics; enzyme activity; Enzymes; Food Microbiology; Fundamental and applied biological sciences. Psychology; gene overexpression; gene transfer; Genes; Genes, Bacterial; Genes, Protozoan; Genetic Engineering; Genetic technics; genetically engineered microorganisms; glucose; lactate-dehydrogenase; Lactobacillus plantarum; Lactobacillus plantarum - enzymology; Lactobacillus plantarum - genetics; Lactococcus lactis; Lactococcus lactis - genetics; Lactococcus lactis - metabolism; leuconostoc; mannitol; Mannitol - metabolism; mannitol 1-phosphatase; mannitol 1-phosphate dehydrogenase; mesenteroides; Methods. Procedures. Technologies; Microbiology; Modification of gene expression level; molecular cloning; Nisin - metabolism; phosphoric monoester hydrolases; Phosphoric Monoester Hydrolases - biosynthesis; Phosphoric Monoester Hydrolases - genetics; Plasmids - genetics; protects; Recombinant Proteins - biosynthesis; Recombinant Proteins - genetics; Sugar Alcohol Dehydrogenases - biosynthesis; Sugar Alcohol Dehydrogenases - genetics; Lactococcus lactis; Lactic acid bacteria; Enzyme; Phosphoric monoester hydrolases; Gene overexpression; Esterases; Mannitol-1-phosphate 5-dehydrogenase; Streptococcaceae; Mannitol-1-phosphatase; Mannitol; Production; Bacteria; Hydrolases; Micrococcales; Metabolic engineering; Oxidoreductases

To achieve high mannitol production by Lactococcus lactis, the mannitol 1-phosphatase gene of Eimeria tenella and the mannitol 1-phosphate dehydrogenase gene mtlD of Lactobacillus plantarum were cloned in the nisin-dependent L. lactis NICE overexpression system. As predicted by a kinetic L. lactis g...