Detection of the ORF1 Gene Is an Indicator of the Possible Isolation of Severe Acute Respiratory Syndrome Coronavirus 2

• Published in: Pathogens (Basel) Vol. 11; no. 3; p. 302
• Main Authors: Shirato, Kazuya, Kakizaki, Masatoshi, Tomita, Yuriko, Kawase, Miyuki, Takeda, Makoto
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 27.02.2022; MDPI
• Subjects: Assaying; coronavirus diseases 19 (COVID-19); Coronaviruses; COVID-19; COVID-19 infection; detection limit; Diagnostic systems; Efficiency; genes; Genomes; genomics; Infectivity; nucleocapsid; Nucleocapsids; Orf1 gene; ORF1a; pandemic; Pandemics; pathogenicity; patients; Polymerase chain reaction; proteinases; Proteins; Real time; real-time RT-PCR; Respiratory diseases; RNA; serine; Severe acute respiratory syndrome coronavirus 2; severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2); Surveillance; Viral diseases; viral genome; virus isolation; Viruses; United States > US; Japan; virus isolation; coronavirus diseases 19 (COVID-19); ORF1a; real-time RT-PCR; severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)

In the ongoing coronavirus diseases 2019 (COVID-19) pandemic, caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), real-time RT-PCR based diagnostic assays have been used for the detection of infection, but the positive signal of real-time RT-PCR does not necessarily indicate the...