Protease inhibition and absorption enhancement by functional nanoparticles for effective oral insulin delivery

• Published in: Biomaterials Vol. 33; no. 9; pp. 2801 - 2811
• Main Authors: Su, Fang-Yi, Lin, Kun-Ju, Sonaje, Kiran, Wey, Shiaw-Pyng, Yen, Tzu-Chen, Ho, Yi-Cheng, Panda, Nilendu, Chuang, Er-Yuan, Maiti, Barnali, Sung, Hsing-Wen
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Ltd 01.03.2012
• Subjects: absorption; Absorption - drug effects; Adherens junction; Administration, Oral; Advanced Basic Science; Animals; bioavailability; Biological Availability; biopharmaceuticals; bladder; blood glucose; Blood Glucose - drug effects; Cell Adhesion - drug effects; chitosan; Chitosan - chemical synthesis; Chitosan - chemistry; Complexing agent; confocal microscopy; Dentistry; Diabetes Mellitus, Experimental - blood; Diabetes Mellitus, Experimental - pathology; Drug Delivery Systems - methods; Electric Impedance; enzyme inhibition; Injections, Subcutaneous; insulin; Insulin - administration & dosage; Insulin - blood; Insulin - pharmacokinetics; Insulin - pharmacology; kidneys; metal ions; mixing; Molecular dynamic simulation; Molecular Dynamics Simulation; nanoparticles; Nanoparticles - chemistry; Oral protein delivery; Particle Size; Pentetic Acid - chemistry; permeability; Polyglutamic Acid - chemical synthesis; Polyglutamic Acid - chemistry; Protease Inhibitors - pharmacology; Protective Agents - pharmacology; proteinases; Proteolytic inhibition; Rats; Rats, Wistar; scintigraphy; small intestine; Spectroscopy, Fourier Transform Infrared; Static Electricity; tight junctions; Tissue Distribution - drug effects; Tomography, Emission-Computed, Single-Photon; Tomography, X-Ray Computed; Proteolytic inhibition; Oral protein delivery; Molecular dynamic simulation; Complexing agent; Adherens junction

Complexing agents such as diethylene triamine pentaacetic acid (DTPA) are known to disrupt intestinal tight junctions and inhibit intestinal proteases by chelating divalent metal ions. This study attempts to incorporate these benefits of DTPA in functional nanoparticles (NPs) for oral insulin delive...