Tick Surveillance for Relapsing Fever Spirochete Borrelia miyamotoi in Hokkaido, Japan

• Published in: PloS one Vol. 9; no. 8; p. e104532
• Main Authors: Takano, Ai, Toyomane, Kochi, Konnai, Satoru, Ohashi, Kazuhiko, Nakao, Minoru, Ito, Takuya, Andoh, Masako, Maeda, Ken, Watarai, Masahisa, Sato, Kozue, Kawabata, Hiroki
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 11.08.2014; Public Library of Science (PLoS)
• Subjects: Amblyomma; Animals; Arachnids; Bacteriology; Biology and Life Sciences; Bites; Borrelia; Borrelia - classification; Borrelia - genetics; Borrelia - isolation & purification; Borrelia burgdorferi; Borrelia miyamotoi; Campylobacter; Deoxyribonucleic acid; Disease control; DNA; DNA, Bacterial - genetics; DNA, Bacterial - isolation & purification; Environmental Monitoring; Epidemiology; Female; Fever; Genes; Health surveillance; Human behavior; Humans; Infections; Infectious diseases; Insect bites; Japan; Laboratories; Lyme disease; Lysates; Male; Medicine and Health Sciences; Phylogeny; Public health; Relapsing fever; Relapsing Fever - microbiology; Relapsing Fever - transmission; Ribosomal DNA; rRNA 16S; Science; Spirochetes; Ticks; Ticks - microbiology; Ticks - physiology; Vector-borne diseases; Veterinary colleges; Veterinary medicine; Japan; North America; Europe; Russia

During 2012-2013, a total of 4325 host-seeking adult ticks belonging to the genus Ixodes were collected from various localities of Hokkaido, the northernmost island of Japan. Tick lysates were subjected to real-time PCR assay to detect borrelial infection. The assay was designed for specific detecti...