Quantification of Mesenchymal Stem Cell (MSC) Delivery to a Target Site Using In Vivo Confocal Microscopy

• Published in: PloS one Vol. 8; no. 10; p. e78145
• Main Authors: Mortensen, Luke J., Levy, Oren, Phillips, Joseph P., Stratton, Tara, Triana, Brian, Ruiz, Juan P., Gu, Fangqi, Karp, Jeffrey M., Lin, Charles P.
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 29.10.2013; Public Library of Science (PLoS)
• Subjects: Animals; Automation; Biology; Cell number; Cells, Cultured; Confocal microscopy; Dyes; Engineering; Fluorescence; Health sciences; Hospitals; In vivo methods and tests; Inflammation; Interferon; Interferon-beta - pharmacology; Medical schools; Medicine; Mesenchymal stem cells; Mesenchymal Stromal Cells - cytology; Mesenchymal Stromal Cells - drug effects; Mesenchymal Stromal Cells - physiology; Mesenchyme; Mice; Microscopy; Microscopy, Confocal - methods; Optimization; Protein expression; Proteins; Quantum dots; Rodents; Stains & staining; Stem cell transplantation; Stem cells; Therapy; Tissues; Tumor Necrosis Factor-alpha - pharmacology; Tumor necrosis factor-α; Womens health; γ-Interferon; United States > US; Massachusetts

The ability to deliver cells to appropriate target tissues is a prerequisite for successful cell-based therapy. To optimize cell therapy it is therefore necessary to develop a robust method of in vivo cell delivery quantification. Here we examine Mesenchymal Stem Cells (MSCs) labeled with a series o...