Multiplex qPCR for Detection and Absolute Quantification of Malaria

• Published in: PloS one Vol. 8; no. 8; p. e71539
• Main Authors: Kamau, Edwin, Alemayehu, Saba, Feghali, Karla C., Saunders, David, Ockenhouse, Christian F.
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 29.08.2013; Public Library of Science (PLoS)
• Subjects: Assaying; Automation; Biology; Deoxyribonucleic acid; DNA; Ethics; Human subjects; Humans; Malaria; Malaria - diagnosis; Malaria - parasitology; Medical research; Medicine; Microscopy; Multiplex Polymerase Chain Reaction - methods; Multiplex Polymerase Chain Reaction - standards; Multiplexing; Parasite Load; Parasites; Plasmids; Plasmodium; Plasmodium - genetics; Plasmodium falciparum; Plasmodium falciparum - genetics; Plasmodium vivax - genetics; Polymerase chain reaction; Purification; Real-Time Polymerase Chain Reaction - methods; Real-Time Polymerase Chain Reaction - standards; Reproducibility of Results; Sensitivity and Specificity; Studies; Titration; Vector-borne diseases; Cambodia; Thailand; United States > US; Maryland

We describe development of an absolute multiplex quantitative real-time PCR for detection of Plasmodium spp., P. falciparum and P. vivax targets in order to produce an assay amenable to high throughput but with reduced costs. Important qPCR experimental details and information that is critical to pe...