Mesenchymal stromal cell proliferation, gene expression and protein production in human platelet-rich plasma-supplemented media

• Published in: PloS one Vol. 9; no. 8; p. e104662
• Main Authors: Amable, Paola Romina, Teixeira, Marcus Vinicius Telles, Carias, Rosana Bizon Vieira, Granjeiro, José Mauro, Borojevic, Radovan
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 12.08.2014; Public Library of Science (PLoS)
• Subjects: Adipogenesis - drug effects; Adipogenesis - genetics; Adipose tissue; Angiogenesis; Antigens, Surface - metabolism; Biology and Life Sciences; Biomarkers - metabolism; Biomedical materials; Blood platelets; Bone marrow; Calcium; Cell culture; Cell differentiation; Cell Differentiation - drug effects; Cell Differentiation - genetics; Cell Proliferation; Cell surface; Chemokines; Clinical trials; Culture media; Culture Media - pharmacology; Differentiation (biology); Dose-Response Relationship, Drug; Extracellular matrix; Gene Expression; Gene Expression Profiling; Growth factors; Humans; Inflammation; Mesenchymal Stromal Cells - cytology; Mesenchymal Stromal Cells - drug effects; Mesenchymal Stromal Cells - metabolism; Mesenchyme; Osteogenesis - drug effects; Osteogenesis - genetics; Platelet-Rich Plasma; Platelets; Protein Biosynthesis; Proteins; Stem cells; Stromal cells; Supplements; Surface markers; Tissues; Brazil; Rio de Janeiro Brazil

Platelet-rich plasma (PRP) is increasingly used as a cell culture supplement, in order to reduce the contact of human cells with animal-derived products during in vitro expansion. The effect of supplementation changes on cell growth and protein production is not fully characterized. Human mesenchyma...