Mesenchymal stromal cell proliferation, gene expression and protein production in human platelet-rich plasma-supplemented media

Platelet-rich plasma (PRP) is increasingly used as a cell culture supplement, in order to reduce the contact of human cells with animal-derived products during in vitro expansion. The effect of supplementation changes on cell growth and protein production is not fully characterized. Human mesenchyma...

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Published inPloS one Vol. 9; no. 8; p. e104662
Main Authors Amable, Paola Romina, Teixeira, Marcus Vinicius Telles, Carias, Rosana Bizon Vieira, Granjeiro, José Mauro, Borojevic, Radovan
Format Journal Article
LanguageEnglish
Published United States Public Library of Science 12.08.2014
Public Library of Science (PLoS)
Subjects
Adipogenesis - drug effects
Adipogenesis - genetics
Adipose tissue
Angiogenesis
Antigens, Surface - metabolism
Biology and Life Sciences
Biomarkers - metabolism
Biomedical materials
Blood platelets
Bone marrow
Calcium
Cell culture
Cell differentiation
Cell Differentiation - drug effects
Cell Differentiation - genetics
Cell Proliferation
Cell surface
Chemokines
Clinical trials
Culture media
Culture Media - pharmacology
Differentiation (biology)
Dose-Response Relationship, Drug
Extracellular matrix
Gene Expression
Gene Expression Profiling
Growth factors
Humans
Inflammation
Mesenchymal Stromal Cells - cytology
Mesenchymal Stromal Cells - drug effects
Mesenchymal Stromal Cells - metabolism
Mesenchyme
Osteogenesis - drug effects
Osteogenesis - genetics
Platelet-Rich Plasma
Platelets
Protein Biosynthesis
Proteins
Stem cells
Stromal cells
Supplements
Surface markers
Tissues
Brazil
Rio de Janeiro Brazil
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Abstract Platelet-rich plasma (PRP) is increasingly used as a cell culture supplement, in order to reduce the contact of human cells with animal-derived products during in vitro expansion. The effect of supplementation changes on cell growth and protein production is not fully characterized. Human mesenchymal stromal cells from bone marrow, adipose tissue and Wharton's Jelly were isolated and cultured in PRP-supplemented media. Proliferation, in vitro differentiation, expression of cell surface markers, mRNA expression of key genes and protein secretion were quantified. 10% PRP sustained five to tenfold increased cell proliferation as compared to 10% fetal bovine serum. Regarding cell differentiation, PRP reduced adipogenic differentiation and increased calcium deposits in bone marrow and adipose tissue-mesenchymal stromal cells. Wharton's Jelly derived mesenchymal stromal cells secreted higher concentrations of chemokines and growth factors than other mesenchymal stromal cells when cultured in PRP-supplemented media. Bone marrow derived mesenchymal stromal cells secreted higher concentrations of pro-inflammatory and pro-angiogenic proteins. Mesenchymal stromal cells isolated from adipose tissue secreted higher amounts of extracellular matrix components. Mesenchymal stromal cells purified from different tissues have distinct properties regarding differentiation, angiogenic, inflammatory and matrix remodeling potential when cultured in PRP supplemented media. These abilities should be further characterized in order to choose the best protocols for their therapeutic use.
AbstractList BACKGROUND: Platelet-rich plasma (PRP) is increasingly used as a cell culture supplement, in order to reduce the contact of human cells with animal-derived products during in vitro expansion. The effect of supplementation changes on cell growth and protein production is not fully characterized. METHODS: Human mesenchymal stromal cells from bone marrow, adipose tissue and Wharton's Jelly were isolated and cultured in PRP-supplemented media. Proliferation, in vitro differentiation, expression of cell surface markers, mRNA expression of key genes and protein secretion were quantified. RESULTS: 10% PRP sustained five to tenfold increased cell proliferation as compared to 10% fetal bovine serum. Regarding cell differentiation, PRP reduced adipogenic differentiation and increased calcium deposits in bone marrow and adipose tissue-mesenchymal stromal cells. Wharton's Jelly derived mesenchymal stromal cells secreted higher concentrations of chemokines and growth factors than other mesenchymal stromal cells when cultured in PRP-supplemented media. Bone marrow derived mesenchymal stromal cells secreted higher concentrations of pro-inflammatory and pro-angiogenic proteins. Mesenchymal stromal cells isolated from adipose tissue secreted higher amounts of extracellular matrix components. CONCLUSIONS: Mesenchymal stromal cells purified from different tissues have distinct properties regarding differentiation, angiogenic, inflammatory and matrix remodeling potential when cultured in PRP supplemented media. These abilities should be further characterized in order to choose the best protocols for their therapeutic use.
Platelet-rich plasma (PRP) is increasingly used as a cell culture supplement, in order to reduce the contact of human cells with animal-derived products during in vitro expansion. The effect of supplementation changes on cell growth and protein production is not fully characterized. Human mesenchymal stromal cells from bone marrow, adipose tissue and Wharton's Jelly were isolated and cultured in PRP-supplemented media. Proliferation, in vitro differentiation, expression of cell surface markers, mRNA expression of key genes and protein secretion were quantified. 10% PRP sustained five to tenfold increased cell proliferation as compared to 10% fetal bovine serum. Regarding cell differentiation, PRP reduced adipogenic differentiation and increased calcium deposits in bone marrow and adipose tissue-mesenchymal stromal cells. Wharton's Jelly derived mesenchymal stromal cells secreted higher concentrations of chemokines and growth factors than other mesenchymal stromal cells when cultured in PRP-supplemented media. Bone marrow derived mesenchymal stromal cells secreted higher concentrations of pro-inflammatory and pro-angiogenic proteins. Mesenchymal stromal cells isolated from adipose tissue secreted higher amounts of extracellular matrix components. Mesenchymal stromal cells purified from different tissues have distinct properties regarding differentiation, angiogenic, inflammatory and matrix remodeling potential when cultured in PRP supplemented media. These abilities should be further characterized in order to choose the best protocols for their therapeutic use.
Background Platelet-rich plasma (PRP) is increasingly used as a cell culture supplement, in order to reduce the contact of human cells with animal-derived products during in vitro expansion. The effect of supplementation changes on cell growth and protein production is not fully characterized. Methods Human mesenchymal stromal cells from bone marrow, adipose tissue and Wharton's Jelly were isolated and cultured in PRP-supplemented media. Proliferation, in vitro differentiation, expression of cell surface markers, mRNA expression of key genes and protein secretion were quantified. Results 10% PRP sustained five to tenfold increased cell proliferation as compared to 10% fetal bovine serum. Regarding cell differentiation, PRP reduced adipogenic differentiation and increased calcium deposits in bone marrow and adipose tissue-mesenchymal stromal cells. Wharton's Jelly derived mesenchymal stromal cells secreted higher concentrations of chemokines and growth factors than other mesenchymal stromal cells when cultured in PRP-supplemented media. Bone marrow derived mesenchymal stromal cells secreted higher concentrations of pro-inflammatory and pro-angiogenic proteins. Mesenchymal stromal cells isolated from adipose tissue secreted higher amounts of extracellular matrix components. Conclusions Mesenchymal stromal cells purified from different tissues have distinct properties regarding differentiation, angiogenic, inflammatory and matrix remodeling potential when cultured in PRP supplemented media. These abilities should be further characterized in order to choose the best protocols for their therapeutic use.
Author Amable, Paola Romina
Borojevic, Radovan
Teixeira, Marcus Vinicius Telles
Carias, Rosana Bizon Vieira
Granjeiro, José Mauro
AuthorAffiliation 2 National Institute of Metrology, Quality and Technology, Xerém, Rio de Janeiro, Brazil
1 Excellion Biomedical Services S.A., Petrópolis, Rio de Janeiro, Brazil
National Institutes of Health, United States of America
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Copyright 2014 Amable et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.
2014 Amable et al 2014 Amable et al
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Notes Competing Interests: PRA, MVVT, RBVC and RB were employees at Excellion Biomedical Services S.A. This does not alter the authors' adherence to PLOS ONE policies on sharing data and materials. Results reported here have no connections or influence on company 's products. The authors declare that no competing financial and/or private interests exist.
Conceived and designed the experiments: PRA MVTT RBVC JMG RB. Performed the experiments: PRA MVTT RBVC. Analyzed the data: PRA MVTT RB. Contributed reagents/materials/analysis tools: JMG RB. Contributed to the writing of the manuscript: PRA MVTT RBVC JMG RB. Manipulated human samples: RBVC.
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SSID ssj0053866
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Snippet Platelet-rich plasma (PRP) is increasingly used as a cell culture supplement, in order to reduce the contact of human cells with animal-derived products during...
Background Platelet-rich plasma (PRP) is increasingly used as a cell culture supplement, in order to reduce the contact of human cells with animal-derived...
BACKGROUND: Platelet-rich plasma (PRP) is increasingly used as a cell culture supplement, in order to reduce the contact of human cells with animal-derived...
Background Platelet-rich plasma (PRP) is increasingly used as a cell culture supplement, in order to reduce the contact of human cells with animal-derived...
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StartPage e104662
SubjectTerms Adipogenesis - drug effects
Adipogenesis - genetics
Adipose tissue
Angiogenesis
Antigens, Surface - metabolism
Biology and Life Sciences
Biomarkers - metabolism
Biomedical materials
Blood platelets
Bone marrow
Calcium
Cell culture
Cell differentiation
Cell Differentiation - drug effects
Cell Differentiation - genetics
Cell Proliferation
Cell surface
Chemokines
Clinical trials
Culture media
Culture Media - pharmacology
Differentiation (biology)
Dose-Response Relationship, Drug
Extracellular matrix
Gene Expression
Gene Expression Profiling
Growth factors
Humans
Inflammation
Mesenchymal Stromal Cells - cytology
Mesenchymal Stromal Cells - drug effects
Mesenchymal Stromal Cells - metabolism
Mesenchyme
Osteogenesis - drug effects
Osteogenesis - genetics
Platelet-Rich Plasma
Platelets
Protein Biosynthesis
Proteins
Stem cells
Stromal cells
Supplements
Surface markers
Tissues
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Title Mesenchymal stromal cell proliferation, gene expression and protein production in human platelet-rich plasma-supplemented media
URI https://www.ncbi.nlm.nih.gov/pubmed/25115920
https://www.proquest.com/docview/2013761385
https://pubmed.ncbi.nlm.nih.gov/PMC4130592
https://doaj.org/article/cd1d87194d80469db93770150878450f
http://dx.doi.org/10.1371/journal.pone.0104662
Volume 9
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