Novel flowcytometry-based approach of malignant cell detection in body fluids using an automated hematology analyzer

• Published in: PloS one Vol. 13; no. 2; p. e0190886
• Main Authors: Ai, Tomohiko, Tabe, Yoko, Takemura, Hiroyuki, Kimura, Konobu, Takahashi, Toshihiro, Yang, Haeun, Tsuchiya, Koji, Konishi, Aya, Uchihashi, Kinya, Horii, Takashi, Ohsaka, Akimichi
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 09.02.2018; Public Library of Science (PLoS)
• Subjects: Algorithms; Biology and Life Sciences; Body fluids; Cellular biology; Cerebrospinal fluid; Channel gating; Differentiation; Flow cytometry; Fluids; Fluorescence; Gene expression; Granule cells; Hematology; Laboratories; Leukocytes; Leukocytes (mononuclear); Leukocytes (neutrophilic); Malignancy; Medical diagnosis; Medicine; Medicine and Health Sciences; Microscopy; Monocytes; Pathology; Physical Sciences; Research and Analysis Methods; Sensitivity; Solid tumors; Stem cells; Studies; Tumor cells; University graduates; Japan

Morphological microscopic examinations of nucleated cells in body fluid (BF) samples are performed to screen malignancy. However, the morphological differentiation is time-consuming and labor-intensive. This study aimed to develop a new flowcytometry-based gating analysis mode "XN-BF gating alg...