Combination of CD157 and FLAER to Detect Peripheral Blood Eosinophils by Multiparameter Flow Cytometry

• Published in: Journal of Clinical and Experimental Hematopathology Vol. 55; no. 2; pp. 55 - 60
• Main Authors: Marini, Alessandra, Domenichini, Cristiana, Ottaviano, Virginia, Petrini, Mario, Pacini, Simone, Carulli, Giovanni, Sammuri, Paola
• Format: Journal Article
• Language: English
• Published: Japan The Japanese Society for Lymphoreticular Tissue Research 2015
• Subjects: ADP-ribosyl Cyclase - chemistry; Antigens, CD - chemistry; Antigens, CD - metabolism; Antigens, Neoplasm - metabolism; Bacterial Toxins - chemistry; CD157; CD52 Antigen; eosinophils; Eosinophils - cytology; Eosinophils - metabolism; Female; FLAER; Flow Cytometry - methods; Glycoproteins - metabolism; GPI-Linked Proteins - chemistry; GPI-Linked Proteins - metabolism; Humans; Male; multiparameter flow cytometry; Pore Forming Cytotoxic Proteins - chemistry; Receptors, IgG - metabolism

The identification of eosinophils by flow cytometry is difficult because most of the surface antigens expressed by eosinophils are shared with neutrophils. Some methods have been proposed, generally based on differential light scatter properties, enhanced autofluorescence, lack of CD16 or selective...