Number and Density of AMPA Receptors in Single Synapses in Immature Cerebellum

• Published in: The Journal of neuroscience Vol. 25; no. 4; pp. 799 - 807
• Main Authors: Tanaka, Jun-ichi, Matsuzaki, Masanori, Tarusawa, Etsuko, Momiyama, Akiko, Molnar, Elek, Kasai, Haruo, Shigemoto, Ryuichi
• Format: Journal Article
• Language: English
• Published: United States Soc Neuroscience 26.01.2005; Society for Neuroscience
• Subjects: Animals; Cellular/Molecular; Cerebellum - growth & development; Cerebellum - metabolism; Cerebellum - ultrastructure; Excitatory Postsynaptic Potentials - drug effects; Excitatory Postsynaptic Potentials - physiology; Freeze Fracturing - methods; Glutamates; Immunohistochemistry; In Vitro Techniques; Indoles; Kinetics; Purkinje Cells - physiology; Rats; Rats, Sprague-Dawley; Receptors, AMPA - metabolism; Synapses - metabolism; Synapses - physiology

The number of ionotropic receptors in synapses is an essential factor for determining the efficacy of fast transmission. We estimated the number of functional AMPA receptors at single postsynaptic sites by a combination of two-photon uncaging of glutamate and the nonstationary fluctuation analysis i...