Enhanced cell-surface display and secretory production of cellulolytic enzymes with Saccharomyces cerevisiae Sed1 signal peptide

• Published in: Biotechnology and bioengineering Vol. 113; no. 11; pp. 2358 - 2366
• Main Authors: Inokuma, Kentaro, Bamba, Takahiro, Ishii, Jun, Ito, Yoichiro, Hasunuma, Tomohisa, Kondo, Akihiko
• Format: Journal Article
• Language: English
• Published: United States Blackwell Publishing Ltd 01.11.2016; Wiley Subscription Services, Inc
• Subjects: Anchoring; Aspergillus aculeatus; Baking yeast; Bioengineering; Biofuels; Cassettes; Cell Membrane - metabolism; Cell surface; cell surface display; Cell walls; Cellular biology; Cellulase - secretion; Cellulolytic enzymes; endo-glucanase; Endoglucanase; Enzymes; Ethanol; EXG1 protein; Fungi; Gene sequencing; Genes; Genetic Enhancement - methods; Genomes; Glucoamylase; Glucosidase; Glycosylphosphatidylinositol; Hypocrea jecorina; Lignocellulose; Localization; Mating; Membrane Glycoproteins - genetics; Membrane Glycoproteins - metabolism; Peptides; Pichia pastoris; Protein Engineering - methods; Protein Transport - genetics; Proteins; Recombinant; Recombinant Proteins - biosynthesis; Recombinant Proteins - genetics; Rhizopus oryzae; Saccharomyces cerevisiae; Saccharomyces cerevisiae - physiology; Saccharomyces cerevisiae Proteins - genetics; Saccharomyces cerevisiae Proteins - metabolism; Secretion; secretion signal sequence; Surface chemistry; Utilization; Yeast; Yeasts; β-glucosidase; Pichia pastoris; β-glucosidase; endo-glucanase; Saccharomyces cerevisiae; secretion signal sequence; cell surface display

ABSTRACT Recombinant yeast strains displaying aheterologous cellulolytic enzymes on their cell surfaces using a glycosylphosphatidylinositol (GPI) anchoring system are a promising strategy for bioethanol production from lignocellulosic materials. A crucial step for cell wall localization of the enzy...