禾花鲤鱼种暴发性死亡征病原菌的分离鉴定及药敏试验

【目的】明确引起禾花鲤发生暴发性死亡征的病原菌及其耐药性,为有效防控禾花鲤暴发性死亡征提供科学依据。【方法】采用常规方法分离病原菌,以API 20NE生化鉴定与16S r RNA基因序列分析相结合的方法进行鉴定,人工回归感染试验确定分离菌株的致病性,K-B纸片扩散法测定病原菌对药物的敏感性。【结果】从广西全州县患暴发性死亡征禾花鲤的肝脏中分离获得1株优势菌株（TH5）,API 20NE生化鉴定和16S r RNA基因序列分析结果显示,分离菌株TH5为简达气单胞菌（Aeromonas jandaei）,与简达气单胞菌标准菌株ATCC 49568（NR 119040）和CDC0787-80（NR...

Full description

Saved in:
Bibliographic Details
Published in南方农业学报 Vol. 47; no. 7; pp. 1241 - 1246
Main Author 龙苏 邓小红 梁静真 牛志伟 韩书煜 范华龙 黄钧
Format Journal Article
LanguageChinese
Published 广西水生动物病害诊断实验室,南宁530005 2016
全州县水产技术推广站,广西全州541500%广西水产技术推广总站,南宁,530022
广西大学动物科学技术学院/广西高校水生生物健康养殖与营养调控重点实验室,南宁530005%广西水生动物病害诊断实验室,南宁530005
Subjects
分离鉴定
禾花鲤
简达气单胞菌
药敏试验
Aeromonas jandaei
isolation and identification
禾花鲤
Procypris merus
药敏试验
drug sensitivity test
简达气单胞菌
分离鉴定
Online AccessGet full text
ISSN2095-1191
DOI10.3969/j:issn.2095-1191.2016.07.1241

Cover

More Information
Summary:【目的】明确引起禾花鲤发生暴发性死亡征的病原菌及其耐药性,为有效防控禾花鲤暴发性死亡征提供科学依据。【方法】采用常规方法分离病原菌,以API 20NE生化鉴定与16S r RNA基因序列分析相结合的方法进行鉴定,人工回归感染试验确定分离菌株的致病性,K-B纸片扩散法测定病原菌对药物的敏感性。【结果】从广西全州县患暴发性死亡征禾花鲤的肝脏中分离获得1株优势菌株（TH5）,API 20NE生化鉴定和16S r RNA基因序列分析结果显示,分离菌株TH5为简达气单胞菌（Aeromonas jandaei）,与简达气单胞菌标准菌株ATCC 49568（NR 119040）和CDC0787-80（NR 037013）的相似度分别为99.9%和99.6%。人工回归感染试验结果表明,菌株TH5是导致禾花鲤暴发性死亡征的病原菌。该菌株对复方新诺明、环丙沙星、多粘菌素B等11种药物高度敏感,对青霉素G、复达欣、先锋霉素VI等8种药物已产生耐药性。【结论】引起广西全州县养殖禾花鲤暴发性死亡征的病原菌是简达气单胞菌,生产中可选用复方新诺明、环丙沙星、多粘菌素B等药物进行防治。
Bibliography:45-1381/S
Procypris merus; Aeromonas jandaei; isolation and identification; drug sensitivity test
LONG Su, DENG Xiao-hong, LIANG Jing-zhen, NIU Zhi-wei, HAN Shu-yu, FAN Hua-long, HUANG Jun （1Guangxi Aquatic Animal Diseases Diagnosis Laboratory, Nanning 530005, China; 2College of Animal Science and Technology, Guangxi University/Guangxi Colleges and Universities Key Laboratory of Aquatic Healthy Breeding and Nutrition Regulation, Nanning 530005, China; 3Quanzhou County Aquaculture Technology Extending Stations, Quanzhou, Guangxi 541500, China; 4Guangxi Aquaculture Technology Popularization Station, Nanning 530022, China）
[Objective]In order to provide reference for effective prevention and control of fulminant death for Proeypris merus, the present experiment was conducted to investigate pathogen causing fulminant death of P. merus and its drug-resistance. [Method]The pathogen was isolated by conventional method, then the pathogen was identified based on API 20NE biochemical identification and 16S rRNA gene seque
ISSN:2095-1191
DOI:10.3969/j:issn.2095-1191.2016.07.1241