系统性鉴定长非编码RNA MALAT1调控的微小RNA
背景与目的长非编码RNA（long non-coding RNA,lnc RNA）在肿瘤的发生、侵袭转移等过程中发挥着重要的调控作用。本研究通过实验和生物信息学手段系统性研究lnc RNA MALAT1调控的微小RNA（micro RNA,miRNA）。方法设计特异性敲减MALAT1的反义寡核苷酸（antisense oligonucleotides,ASO）,在A549细胞中敲减MALAT1,通过Tqa Man Low Density Array（TLDA）芯片研究敲减MALAT1后miRNA表达变化;使用基因集富集分析（gene set enrichment analysis,GSEA）方...
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| Published in | 中国肺癌杂志 Vol. 19; no. 5; pp. 247 - 251 |
|---|---|
| Main Author | |
| Format | Journal Article |
| Language | Chinese |
| Published |
徐州医科大学临床医学系,徐州,221004%徐州医科大学附属医院心胸外科,徐州,221000%南京医科大学附属江苏省肿瘤医院胸外科/江苏省恶性肿瘤分子生物学及转化医学重点实验室,南京,210009
2016
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| Subjects | |
| Online Access | Get full text |
| ISSN | 1009-3419 1999-6187 |
| DOI | 10.3779/j.issn.1009-3419.2016.05.11 |
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| Abstract | 背景与目的长非编码RNA（long non-coding RNA,lnc RNA）在肿瘤的发生、侵袭转移等过程中发挥着重要的调控作用。本研究通过实验和生物信息学手段系统性研究lnc RNA MALAT1调控的微小RNA（micro RNA,miRNA）。方法设计特异性敲减MALAT1的反义寡核苷酸（antisense oligonucleotides,ASO）,在A549细胞中敲减MALAT1,通过Tqa Man Low Density Array（TLDA）芯片研究敲减MALAT1后miRNA表达变化;使用基因集富集分析（gene set enrichment analysis,GSEA）方法分析敲减MALAT1之后差异表达基因,寻找富集的miRNA。结果 ASO有效降低了MALAT1表达,敲减MALAT1之后153个miRNA表达显著变化,其中131个miRNA表达上调,22个miRNA表达下调。在A549细胞中敲减MALAT1后,458个基因发生显著差异表达,GSEA分析发现多个miRNA在差异表达基因中被显著富集。对TLDA和GSEA数据取交集并进一步分析确认28个被MALAT1调控的miRNA。结论本研究系统鉴定了MALAT1对miRNA的调控,为进一步研究提供了基础。 |
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| AbstractList | 背景与目的长非编码RNA（long non-coding RNA,lnc RNA）在肿瘤的发生、侵袭转移等过程中发挥着重要的调控作用。本研究通过实验和生物信息学手段系统性研究lnc RNA MALAT1调控的微小RNA（micro RNA,miRNA）。方法设计特异性敲减MALAT1的反义寡核苷酸（antisense oligonucleotides,ASO）,在A549细胞中敲减MALAT1,通过Tqa Man Low Density Array（TLDA）芯片研究敲减MALAT1后miRNA表达变化;使用基因集富集分析（gene set enrichment analysis,GSEA）方法分析敲减MALAT1之后差异表达基因,寻找富集的miRNA。结果 ASO有效降低了MALAT1表达,敲减MALAT1之后153个miRNA表达显著变化,其中131个miRNA表达上调,22个miRNA表达下调。在A549细胞中敲减MALAT1后,458个基因发生显著差异表达,GSEA分析发现多个miRNA在差异表达基因中被显著富集。对TLDA和GSEA数据取交集并进一步分析确认28个被MALAT1调控的miRNA。结论本研究系统鉴定了MALAT1对miRNA的调控,为进一步研究提供了基础。 背景与目的长非编码RNA（long non-coding RNA, lncRNA）在肿瘤的发生、侵袭转移等过程中发挥着重要的调控作用。本研究通过实验和生物信息学手段系统性研究lncRNA MALAT1调控的微小RNA（microRNA, miRNA）。方法设计特异性敲减MALAT1的反义寡核苷酸（antisense oligonucleotides, ASO），在A549细胞中敲减MALAT1，通过TqaMan Low Density Array（TLDA）芯片研究敲减MALAT1后miRNA表达变化；使用基因集富集分析（gene set enrichment analysis, GSEA）方法分析敲减MALAT1之后差异表达基因，寻找富集的miRNA。结果 ASO有效降低了MALAT1表达，敲减MALAT1之后153个miRNA表达显著变化，其中131个miRNA表达上调，22个miRNA表达下调。在A549细胞中敲减MALAT1后，458个基因发生显著差异表达，GSEA分析发现多个miRNA在差异表达基因中被显著富集。对TLDA和GSEA数据取交集并进一步分析确认28个被MALAT1调控的miRNA。结论本研究系统鉴定了MALAT1对miRNA的调控，为进一步研究提供了基础。 |
| Abstract_FL | Background and objective Long non-coding RNA (lncRNA) plays important regulatory roles in the development and invasion of various cancers. hTe aim of current study is to comprehensively identify microRNAs (miRNA) regulated by lncRNA MALAT1 via experimental and bioinformatics methods.Methods Antisense oligonucleotides (ASO) speciifcally targeting MALAT1 were designed and synthesized. Atfer knockdown of MALAT1 by ASO in A549 cells, miRNA expression changes were proifled by TqaMan Low Density Array (TLDA). Gene set enrichment analysis (GSEA) was used to search enriched miRNAs among differentially expressed genes atfer knockdown of MALAT1.ResultsAtfer effcient knock-down of MALAT1 by ASO, 153 miRNAs were differentially expressed, 131 up-regulated and 22 down-regulated. Among the 458 differentially expressed genes atfer MALAT1 silence, GSEA results revealed lots of enriched miRNAs. hTere were 28 over-lapped miRNAs between TLDA and GSEA results, suggesting these 28 miRNAs are regulated by MALAT1.Conclusion hTis study comprehensively identiifed MALAT1 regulated miRNAs, providing resources for further research. |
| Author | 张海天 王国祥 尹荣 邱满堂 许林 |
| AuthorAffiliation | 徐州医科大学临床医学系,徐州221004 徐州医科大学附属医院心胸外科,徐州221000 南京医科大学附属江苏省肿瘤医院胸外科／江苏省恶性肿瘤分子生物学及转化医学重点实验室,南京210009 |
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| Author_FL | Rong YIN Guoxiang WANG Lin XU Haitian ZHANG Mantang QIU |
| Author_FL_xml | – sequence: 1 fullname: Haitian ZHANG – sequence: 2 fullname: Guoxiang WANG – sequence: 3 fullname: Rong YIN – sequence: 4 fullname: Mantang QIU – sequence: 5 fullname: Lin XU |
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| Copyright | Copyright © Wanfang Data Co. Ltd. All Rights Reserved. |
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| DOI | 10.3779/j.issn.1009-3419.2016.05.11 |
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| DocumentTitleAlternate | Comprehensive Identification of MicroRNAs Regulated by Long Non-coding RNA MALAT1 |
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| Keywords | 生物信息学 长非编码RNA MicroRNAs microRNA MALAT1 Bioinformatics Long non-coding RNA |
| Language | Chinese |
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| Notes | Haitian ZHANG, Guoxiang WANG, Rong YIN, Mantang QIU, Lin XU;1Department of Clinical Medicine, Xuzhou Medical University, Xuzhou 221004, China; 2Department of Cardiothoracic Surgery, Af- filiated Hospital of Xuzhou Medical University, Xuzhou 221000, China; 3Department of Thoracic Surgery, Nanjing Medical University Affiliated Cancer Hospital, Jiangsu Key Laboratory of Molecular and Translational Cancer Research, Cancer Institute of Jiangsu Province, Nanjing 210009, China Long non-coding RNA; MALAT i; MicroRNAs; Bioinformatics 12-1395/R Background and objective Long non-coding RNA （lncRNA） plays important regulatory roles in the development and invasion of various cancers. The aim of current study is to comprehensively identify microRNAs （miRNA） regulated by lncRNA MALAT 1 via experimental and bioinformatics methods. Methods Antisense oligonucleotides （ASO） specifically targeting MALAT1 were designed and synthesized. After knockdown of MALAT1 by ASO in A549 ceils, miRNA expression changes were profiled by TqaMan L |
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| PublicationTitle | 中国肺癌杂志 |
| PublicationTitleAlternate | Chinese Journal of Lung Cancer |
| PublicationYear | 2016 |
| Publisher | 徐州医科大学临床医学系,徐州,221004%徐州医科大学附属医院心胸外科,徐州,221000%南京医科大学附属江苏省肿瘤医院胸外科/江苏省恶性肿瘤分子生物学及转化医学重点实验室,南京,210009 |
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| Snippet | 背景与目的长非编码RNA（long non-coding RNA,lnc RNA）在肿瘤的发生、侵袭转移等过程中发挥着重要的调控作用。本研究通过实验和生物信息学手段系统性研究lnc RNA MALAT1调控的微小RNA（micro RNA,miRNA）。方法设计特异性敲减MALAT1的反义寡核苷酸（antisense... 背景与目的长非编码RNA（long non-coding RNA, lncRNA）在肿瘤的发生、侵袭转移等过程中发挥着重要的调控作用。本研究通过实验和生物信息学手段系统性研究lncRNA MALAT1调控的微小RNA（microRNA, miRNA）。方法设计特异性敲减MALAT1的反义寡核苷酸（antisense... |
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| SubjectTerms | MALAT1 microRNA 生物信息学 长非编码RNA |
| Title | 系统性鉴定长非编码RNA MALAT1调控的微小RNA |
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