轮枝镰孢菌的绿色荧光蛋白基因标记

本研究采用农杆菌介导法（ATMT）将绿色荧光蛋白基因（gfp）转入轮枝镰孢菌（Fusarium verticillioides）获得了表达gfp的转化子。结果表明,转化子经过5代培养仍能发出稳定的荧光,转化后的菌落形态与菌丝生长速度差异不明显,对寄主仍有致病能力。通过PCR验证也表明gfp基因已成功转入到菌株PX1015基因组中,轮枝镰孢菌绿色荧光蛋白基因转化体的成功构建为该病菌侵染机制的研究奠定了基础。...

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Bibliographic Details
Published in西南农业学报 Vol. 27; no. 6; pp. 2374 - 2376
Main Author 张小飞 李晓 崔丽娜 邹成佳 彭云良 杨晓蓉
Format Journal Article
LanguageChinese
Published 四川省农业科学院植物保护研究所,农业部西南作物有害生物综合治理重点实验室,四川成都610066 2014
Subjects
农杆菌
绿色荧光蛋白
轮枝镰孢菌
遗传转化
GFP
tumefaciens
绿色荧光蛋白
遗传转化
verticillioides
Fusarium
Genetic
Agrobactetium
transformation
农杆菌
轮枝镰孢菌
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ISSN1001-4829
DOI10.16213/j.cnki.scjas.2014.06.022

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Summary:本研究采用农杆菌介导法（ATMT）将绿色荧光蛋白基因（gfp）转入轮枝镰孢菌（Fusarium verticillioides）获得了表达gfp的转化子。结果表明,转化子经过5代培养仍能发出稳定的荧光,转化后的菌落形态与菌丝生长速度差异不明显,对寄主仍有致病能力。通过PCR验证也表明gfp基因已成功转入到菌株PX1015基因组中,轮枝镰孢菌绿色荧光蛋白基因转化体的成功构建为该病菌侵染机制的研究奠定了基础。
Bibliography:51-1213/S
Agrobacterium tumefaciens; Fusarium verticillioides; GFP; Genetic transformation
ZHANG Xiao-fei,LI Xiao,CUI Li-na,ZOU Cheng-jia,PENG Yun-liang,YANG Xiao-rong（ Key Laboratory of Integrated Pest Management on Crops in Southwest, Ministry of Agriculture, Institute of Plant Protection, Sichuan Acad- emy of Agricultural Sciences, Sichuan Chengdu 610066, China）
In this study,transformants of expressing green fluorescent protein was obtained by delivering gfp gene into Fusarium verticillioides via agrobacterium tumefaciens-mediated transformation（ ATMT）. The result showed that the transformants had genetic stability and excellent fluorescence after 5 generations,and there were no significant differences in their colony morphology and mycelium growth rate,whose pathogenicity of host infection remained. PCR detection also indicated that the gfp gene had been transferred to the strain F. verticillioides PX1015. This study would provide a basis for the infection process using the green fluorescent transformant of
ISSN:1001-4829
DOI:10.16213/j.cnki.scjas.2014.06.022