Oriented, Active Escherichia coli RNA Polymerase: An Atomic Force Microscope Study

• Published in: Biophysical journal Vol. 76; no. 2; pp. 1024 - 1033
• Main Authors: Thomson, Neil H., Smith, Bettye L., Almqvist, Nils, Schmitt, Lutz, Kashlev, Mikhail, Kool, Eric T., Hansma, Paul K.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.02.1999; Biophysical Society
• Subjects: Bacteria; Biochemistry; DNA, Single-Stranded - metabolism; DNA-Directed RNA Polymerases - metabolism; DNA-Directed RNA Polymerases - ultrastructure; Enzymes; Escherichia coli - enzymology; Fysik; Gold; Microscopy, Atomic Force - methods; Nitrilotriacetic Acid - metabolism; Physics; Protein Binding; Recombinant Proteins - metabolism; Recombinant Proteins - ultrastructure; Ribonucleic acid; RNA; RNA - analysis; Sulfhydryl Compounds - metabolism; Transcription, Genetic - genetics

Combining a system for binding proteins to surfaces (Sigal, G. B., C. Bamdad, A. Barberis, J. Strominger, and G. M. Whitesides. 1996. Anal. Chem. 68:490–497) with a method for making ultraflat gold surfaces (Hegner, M., P. Wagner, and G. Semenza. 1993. Surface Sci. 291:39–46 1993) has enabled single...