High-throughput immunoglobulin repertoire analysis distinguishes between human IgM memory and switched memory B-cell populations

• Published in: Blood Vol. 116; no. 7; pp. 1070 - 1078
• Main Authors: Wu, Yu-Chang, Kipling, David, Leong, Hui Sun, Martin, Victoria, Ademokun, Alexander A., Dunn-Walters, Deborah K.
• Format: Journal Article
• Language: English
• Published: Washington, DC Elsevier Inc 19.08.2010; Americain Society of Hematology; American Society of Hematology
• Series: Immunobiology
• Subjects: Adult; B-cell receptor; B-Lymphocytes - immunology; Biological and medical sciences; Blood; Cells, Cultured; Clonal selection; Gene Rearrangement; Germinal centers; Heavy chains; Hematologic and hematopoietic diseases; Humans; Immunobiology; Immunoglobulin D - genetics; Immunoglobulin D - immunology; Immunoglobulin M; Immunoglobulin M - genetics; Immunoglobulin M - immunology; Immunoglobulin Variable Region - genetics; Immunoglobulin Variable Region - immunology; Immunoglobulins; Immunologic Memory - physiology; Immunological memory; Leukocytes, Mononuclear; Lymphocytes B; Medical sciences; Memory cells; Polymerase Chain Reaction; somatic hypermutation; Tumor Necrosis Factor Receptor Superfamily, Member 7 - genetics; Tumor Necrosis Factor Receptor Superfamily, Member 7 - immunology; Young Adult; Human; Immunoglobulins; Hematology; B-Lymphocyte; Immunologic repertoire; IgM; Memory lymphocyte

B-cell receptor (BCR) diversity is achieved centrally by rearrangement of Variable, Diversity, and Joining genes, and peripherally by somatic hypermutation and class-switching of the rearranged genes. Peripheral B-cell populations are subject to both negative and positive selection events in the cou...