Chip-based human liver–intestine and liver–skin co-cultures – A first step toward systemic repeated dose substance testing in vitro

• Published in: European journal of pharmaceutics and biopharmaceutics Vol. 95; no. Pt A; pp. 77 - 87
• Main Authors: Maschmeyer, Ilka, Hasenberg, Tobias, Jaenicke, Annika, Lindner, Marcus, Lorenz, Alexandra Katharina, Zech, Julie, Garbe, Leif-Alexander, Sonntag, Frank, Hayden, Patrick, Ayehunie, Seyoum, Lauster, Roland, Marx, Uwe, Materne, Eva-Maria
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.09.2015
• Subjects: 3D tissue culture; Alternatives to animal testing; Cell Line, Tumor; Chromans - administration & dosage; Coculture Techniques - methods; Endothelium, Vascular - drug effects; Endothelium, Vascular - metabolism; Female; Human barrier models; Human-on-a-chip; Humans; Ileum - drug effects; Ileum - metabolism; Intestine model; Lab-On-A-Chip Devices; Liver - drug effects; Liver - metabolism; Microphysiological system; Organ-on-a-chip; Skin - drug effects; Skin - metabolism; Skin equivalent; Systemic drug testing; Thiazolidinediones - administration & dosage; Troglitazone; Vasculature; Young Adult; HHSteC; NaK-ATPase; vWF; DMSO; Human-on-a-chip; Human barrier models; Organ-on-a-chip; SLC5A1; MRP-1; ECGM-MV2; Alternatives to animal testing; CMOS; LC-MS/MS; MPS; RBC; MRP-2; MUC2; OECD; TUNEL; Microphysiological system; SI; Systemic drug testing; PPARg; 3D tissue culture; CYP; PPARa; MOC; BSEP; MMP7; TBP; Skin equivalent; qRT-PCR; ICH; Vasculature; μPIV; ZO-1; HDMEC; TEER; CPS-1; PDMS; Intestine model

[Display omitted] •The MOC allows integration of biological barriers into dynamic multi-tissue systems.•A human primary intestinal and a liver model were combined in a microfluidic system.•Liver and skin models were co-cultured in endothelialized multi-organ-chips.•Oral treatment of co-cultures was...