A genome-wide siRNA screen identifies a druggable host pathway essential for the Ebola virus life cycle

• Published in: Genome medicine Vol. 10; no. 1; pp. 58 - 14
• Main Authors: Martin, Scott, Chiramel, Abhilash I., Schmidt, Marie Luisa, Chen, Yu-Chi, Whitt, Nadia, Watt, Ari, Dunham, Eric C., Shifflett, Kyle, Traeger, Shelby, Leske, Anne, Buehler, Eugen, Martellaro, Cynthia, Brandt, Janine, Wendt, Lisa, Müller, Andreas, Peitsch, Stephanie, Best, Sonja M., Stech, Jürgen, Finke, Stefan, Römer-Oberdörfer, Angela, Groseth, Allison, Feldmann, Heinz, Hoenen, Thomas
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 07.08.2018; BioMed Central; BMC
• Subjects: Animals; Antiviral activity; Antiviral Agents - pharmacology; Biosynthesis; Carbamoyl phosphate synthetase 2 aspartate transcarbamylase and dihydroorotase; Cell cycle; Cell Line, Tumor; Cercopithecus aethiops; Cloning, Molecular; De novo pyrimidine synthesis; Dihydroorotate dehydrogenase; Disease resistance; Ebola virus; Ebolavirus; Ebolavirus - drug effects; Ebolavirus - pathogenicity; Ebolavirus - physiology; Gene Knockdown Techniques; Genes; Genetic aspects; Genetic screening; Genome, Human; Genomes; HEK293 Cells; Host factor; Host-Pathogen Interactions - genetics; Humans; Kinases; Life cycles; Outbreaks; Proteins; Replication; RNA viruses; siRNA; Small interfering RNA; Teriflunomide; Transcription; Vero Cells; Virus Replication; Viruses; West Africa; Sudan; Ebola virus; Non-segmented negative-sense RNA viruses; De novo pyrimidine synthesis; NNSV; Carbamoyl phosphate synthetase 2 aspartate transcarbamylase and dihydroorotase; Teriflunomide; siRNA screen; Host factor; Dihydroorotate dehydrogenase

The 2014-2016 Ebola virus (EBOV) outbreak in West Africa highlighted the need for improved therapeutic options against this virus. Approaches targeting host factors/pathways essential for the virus are advantageous because they can potentially target a wide range of viruses, including newly emerging...